The Bioimaging Facility acts as a support structure to help and nurture research done with Light Microscopy inside the institute. Besides managing resources, the Facility provides iMM scientists and visitors with excellence in scientific know-how and expertise in using light microscopy methods for their research. We assist in planning microscopy-oriented projects, choosing materials and equipment, analyzing experimental results, processing acquired images and presenting data. Together with continuous training of new users, we organize regular courses to introduce users to the most recent microscopy techniques and foster interactions and collaborations between microscopy users at the iMM.
Head of Facility
José Rino has a Licenciatura in Technological Physics Engineering (Instituto Superior Técnico) and a PhD in Biophysics (Faculdade de Ciências). José has managed the Bioimaging Facility since January 2008 and was appointed Head in July 2009, after postdoctoral research at the iMM (Carmo-Fonseca Lab) focused on applying quantitative microscopy methods to study mRNA biogenesis. He is also Head of the Flow Cytometry Facility at iMM. Click here for more information.
Ana Nascimento has a Licenciatura in Chemistry and a MSc in Biochemistry from Faculdade de Ciências. Ana joined the Bioimaging Facility in December 2012 after working as a flow cytometry technician and a microscopy technician at Instituto Gulbenkian de Ciência.
António Temudo has a Licenciatura in Biology and a MSc in Evolutionary and Developmental Biology from Faculdade de Ciências. António joined the Bioimaging Facility in June 2009, after studying the mechanics of somitogenesis in vertebrate embryos (mouse and zebrafish) using confocal live imaging for his MSc thesis. António has also studied Medicine at the Faculdade de Ciências Médicas, Universidade Nova de Lisboa.
The Bioimaging Facility is equipped with 2 inverted laser point-scanning confocal microscopes prepared for live cell imaging (Zeiss LSM 880 and Zeiss LSM 710), 1 lightsheet microscope prepared for water and clearing media immersion imaging (Zeiss Lightsheet Z1), 2 inverted spinning disk confocal microscopes with motorized stage, piezo and EM-CCD also prepared for live cell imaging (3i Marianas SDC and Zeiss Cell Observer SD), 1 multi-photon microscope (Leica SP8 MP), 4 fully motorized inverted widefield microscopes with cooled CCD cameras and incubators (Zeiss Cell Observer, Zeiss Axiovert 200M, Nikon Eclipse Ti and Nikon Eclipse TE-2000-E), 1 upright widefield fluorescence microscope with a CCD camera (Leica DM5000B), 2 fluorescence stereo microscopes with cooled CCD cameras (Zeiss Lumar V12 and Zeiss AxioZoom V16) and other imaging resources such as a brightfield microscope (Leica DM2500) and a bioluminescent imaging system (IVIS Lumina). We also have two high-spec computers for image processing available for researchers to work on their imaging data (Big Guy and Colossus). The Bioimaging systems are available on the online booking system for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience.
We charge according to booked time in each microscopy system. Online booking is mandatory for each usage session and cancellations are allowed if they are made prior to reserved time. Users that do not show up at their booked sessions will be charged in full.
9 a.m. - 6 p.m. for Training, Assistance and Consultations
24 hours, 7 days a week for Equipment Usage by authorized, trained users (see Usage Rules)
All new users must contact the Bioimaging Facility for training in each specific system. This also applies to researchers with previous experience in microscopy. Only trained users are allowed to book usage sessions on a particular microscope.
Instituto de Medicina Molecular, room P2-A-32
Faculdade de Medicina de Lisboa
Av. Prof. Egas Moniz