User Tools
Table of Contents
|
Location: [Lisbon] Room P2-A-24 ( |
47219) 
Nikon Eclipse Ti Quality Control
Nikon Eclipse Ti Usage Statistics![[Lisbon] Room P2-A-24](/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_map_new_p2a24.png){kind=link}
Microscope overview
The Nikon Eclipse Ti is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply, and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using NIS-Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with an Andor Neo 5.5 monochrome cooled sCMOS camera.
- Microscope: Nikon Eclipse Ti
- Camera: Andor Neo 5.5 sCMOS
Data files older than 3 months will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.
System components
Filtersets
| Position | Filterset | Excitation | Dichroic | Emission | Reference |
|---|---|---|---|---|---|
| 1 | Empty | ||||
| 2 | Blue | 340-380 nm | 400 nm | 435-485 nm | MBE41300 |
| 3 | Green | 465-495 nm | 505 nm | 515-555 nm | MBE44720 |
| 4 | Orange | 528-553 nm | 565 nm | 590-650 nm | MBE45600 |
| 5 | Red | 608-648 nm | 660 nm | 672-712 nm | 2SK4CY50 |
| 6 | Empty | ||||
Objectives
| Magnification | Model | Immersion | NA | WD (mm) | Reference |
|---|---|---|---|---|---|
| 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | MRH10101 |
| 20x | CFI S Plan Fluor ELWD ADM Ph1 | Air | 0.45 | 6.90 - 8.20 | MRH48230 |
| 40x | Plan Apo DM Ph3 | Oil | 1.00 | 0.16 | MRD31400 |
| 40x | Plan Fluor DLL Ph2 | Air | 0.75 | 0.72 | MRH10400 |
| 40x | Plan Apo M N2 DIC | Air | 0.95 | 0.14 | MRD00400 |
| 40x | Achromat LWD ADL Ph1 | Air | 0.55 | 2.10 | MRP46402 |
Upon request:
| Magnification | Model | Immersion | NA | WD (mm) | Reference |
|---|---|---|---|---|---|
| 100x | CFI Plan | Water | 1.10 | 2.50 | MRL07920 |
| 100x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 |
Camera
| Model | Type | Frame Size | Pixel Size (µm) |
|---|---|---|---|
| Andor Neo 5.5 sCMOS | CCD | 2560 x 2160 | 6.5 x 6.5 |
