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nikon_eclipse_ti

Location: Room P2-A-24 ( 47219)
Manufacturer: Nikon Instruments
Model: Eclipse Ti
Nickname: "006"
Software: NIS-Elements AR 5.11.01
Year: 2010
SN: 602805
 
 
 
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Microscope overview

The Nikon Eclipse Ti is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Andor Neo 5.5 monochrome cooled sCMOS camera ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use PPBI Huygens RM deconvolution server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners Zeiss LSM 710 and Zeiss LSM 880 or spinning disks 3i Marianas SDC and Zeiss Cell Observer SD. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus.

Data files older than 3 months will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

System components

Filtersets

Position Filterset Reference Excitation Dichroic Emission
1 Empty
2 DAPI MBE41300 340-380 nm 400 nm 435-485 nm
3 FITC MBE44720 465-495 nm 505 nm 515-555 nm
4 TRITC MBE45600 528-553 nm 565 nm 590-650 nm
5 Cy5 2SK4CY50 608-648 nm 660 nm 672-712 nm
6 Empty

Objectives

Magnification Model Immersion NA WD (mm) Reference
10x CFI Plan Fluor DLL Ph1 Air 0.30 16.00 MRH10101
20x CFI S Plan Fluor ELWD ADM Ph1 Air 0.45 6.90 - 8.20 MRH48230
40x CFI S Plan Fluor ELWD ADM Ph2 Air 0.60 2.80 - 3.60 MRH48430
40x Plan Apo DM Ph3 Oil 1.00 0.16

Upon request:

Magnification Model Immersion NA WD (mm) pixel size* (μm) 10 μm = Reference
40x Plan Fluor ELWD ADL Ph2 Air 0.60 2.70 - 3.70 0.113 88 pixels

* pixel sizes for binning = 1

Camera

Model Type Frame Size Pixel Size (µm)
Andor Neo 5.5 sCMOS CCD 2560 x 2160 6.5 x 6.5

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nikon_eclipse_ti.txt · Last modified: 2021/10/07 17:19 by bioimaging