Table of Contents
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Location: Room P2-A-24 ( |
47219) 
Nikon Eclipse Ti Quality Control
Nikon Eclipse Ti Usage Statistics
Microscope overview
The Nikon Eclipse Ti is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply, and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using NIS-Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with an Andor Neo 5.5 monochrome cooled sCMOS camera.
- Microscope: Nikon Eclipse Ti
- Camera: Andor Neo 5.5 sCMOS
This microscope is also equipped with a Ionoptix Calcium and Contractility system for high-speed simultaneous acquisition of Fura-2 fluorescence with video-based cell length measurement to study myocyte function, contracting spontaneously or with field stimulation.
- High-speed contractility camera: MyoCam-S3
- Electrical Field Stimulator: MyoPacer
- LED Light Source: UV LEDs for Fura-2 excitation
- High-speed PMT: PMT400 for photon counting at 1kHz
- Software: IonWizard 7.7.3.180
Data files older than 3 months will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
System components
Filtersets Turret 1 (top)
| Position | Filterset | Reference | Excitation | Dichroic | Emission |
|---|---|---|---|---|---|
| 1 | Empty | ||||
| 2 | DAPI | MBE41300 | 340-380 nm | 400 nm | 435-485 nm |
| 3 | FITC | MBE44720 | 465-495 nm | 505 nm | 515-555 nm |
| 4 | TRITC | MBE45600 | 528-553 nm | 565 nm | 590-650 nm |
| 5 | Cy5 | 2SK4CY50 | 608-648 nm | 660 nm | 672-712 nm |
| 6 | Empty | ||||
Filtersets Turret 2 (bottom)
| Position | Filterset |
|---|---|
| 1 | Empty |
| 2 | Empty |
| 3 | Empty |
| 4 | Empty |
| 5 | Empty |
| 6 | Fura-2 |
Objectives
| Magnification | Model | Immersion | NA | WD (mm) | Reference |
|---|---|---|---|---|---|
| 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | MRH10101 |
| 20x | CFI S Plan Fluor ELWD ADM Ph1 | Air | 0.45 | 6.90 - 8.20 | MRH48230 |
| 40x | CFI CFI Plan Apochromat Lambda D | Air | 0.95 | 0.21 | MRH70470 |
| 40x | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | MRH48430 |
| 60x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | |
| 100x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 |
Upon request:
| Magnification | Model | Immersion | NA | WD (mm) | Reference |
|---|---|---|---|---|---|
| 100x | CFI Plan | Water | 1.10 | 2.50 | MRL07920 |
Camera
| Model | Type | Frame Size | Pixel Size (µm) |
|---|---|---|---|
| Andor Neo 5.5 sCMOS | CCD | 2560 x 2160 | 6.5 x 6.5 |
