User Tools

Site Tools


zeiss_celldiscoverer_7

Location: Room P0-A-30 ( 47219)
Manufacturer: ZEISS Microscopy
Model: Celldiscoverer 7 with LSM 900
Nickname: "CD7"
Software: ZEN 3.8 (ZEN celldiscoverer)
Year: 2022
SN: 4648000055
 
Data will be deleted after: 1 month  
 
 
Usage Statistics

Microscope overview

The Zeiss Celldiscoverer 7 is a fully integrated and motorized high-end inverted widefield and point scanning confocal fluorescence imaging system ideal for automated live-cell imaging in multi-well plates and Petri dishes. It offers a fully integrated incubation system with temperature and CO2 control and Definite Focus.3 for hardware focus control. The ZEN software can automatically calibrate the system and adapt it to your sample by recognizing different sample carriers, determining their bottom thickness and material and adjusting the objectives correction ring to compensate for spherical aberrations. You can use Petri dishes, chamber slides, multi-well plates, plastic or glass, thin or thick vessel bottoms, low-skirt or high-skirt plates. When using the 50x water immersion objective, automated immersion supply and removal is available for time lapse experiments over several days or extensive scanning processes on multi-well plates. In widefield mode, the fluorescence excitation unit combines seven LEDs – from deep blue to far-red - for high-speed multi-channel imaging and its sensitive cooled Axiocam 712 camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. In confocal mode, the LSM 900 with Airyscan 2 allows for 3D imaging with increased resolution (120 nm laterally and 350 nm axially in SR mode, at 488 nm) and photomanipulation for FRAP, FRET and related techniques. It can also be used in Multiplex Mode, which enables parallel excitation and detection in 2Y mode (speed improvement of 2x maintaining Airyscan sensitivity).

Data files older than 1 month will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

Booking Rules

  1. Users can book at maximum 8 hours per week
  2. This usage restriction does not apply for weekends and for working days before 9:00 and after 21:00
  3. Exceptions to these rules require approval from José Rino.

System components

LASERs (confocal)

Laser Unit Wavelength Maximum Power
405 405 nm 5 mW
488 488 nm 10 mW
561 561 nm 10 mW
640 640 nm 5 mW

LEDs (widefield)

Line Wavelength
UV 385 nm
V 420 nm
B 470 nm
C 520 nm
G 567 nm
Y 590 nm
R 625 nm

Filtersets (widefield)

Position Filterset Reference Dichroic Emission
1 RQBS FS90HE 405 nm 410-440 nm
493 nm 499-529 nm
575 nm 580-605 nm
653 nm 659-759 nm
2 RTBS FS91HE 450 nm 455-479 nm
538 nm 543-568 nm
610 nm 615-760 nm
3 RTBS FS92HE 405 nm 410-440 nm
493 nm 499-549 nm
610 nm 616-761 nm
4 RDBS FS93HE 493 nm 498-530 nm
575 nm 580-630 nm

Objectives

Model Immersion WD (mm) NA Magnification changer Magnification
Plan-Apochromat 5x / 0.35 Air 5.10 0.12 0.5x 2.5x
0.25 1x 5x
0.35 2x 10x
Plan-Apochromat 20x / 0.7 Air 2.20 0.35 0.5x 10x
0.70 1x 20x
2x 40x
Plan-Apochromat 20x / 0.95 Air 0.76 0.50 0.5x 10x
0.80 1x 20x
0.95 2x 40x
Plan-Apochromat 50x / 1.2 W Water 0.84 1.20 0.5x 25x
1x 50x
2x 100x

Camera (widefield)

Model Type Frame Size Pixel Size (µm)
Axiocam 712 mono CMOS CMOS 4096 x 3008 3.45 x 3.45

How to start your session

The system should be always on, you only need to:

  • Activate the CD7 by pressing the “Standby” button (to the left of the touch panel)

  • Turn on the computer
  • Log into Windows with your Agendo credentials
  • Start ZEN
  • Open the Main valve of the CO2 tank (if needed) - do not change the pressure settings!

How to end your session

If there is another user for this microscope in the next hour:

  • Exit the ZEN software, leave the lasers on (if used)
  • Log off the computer

Else:

  • Close the main valve of the CO2 tank (if used)
  • Turn off the lasers on ZEN
  • Exit the ZEN software
  • Shutdown the computer
  • Press the “Standby” button (to the left of the touch panel).

Microscope Turn On Procedure (after complete shutdown)

If the system is completely off, please follow this procedure:

  • Turn on the MAIN SWITCH (bottom right of the CD7)

  • Activate the CD7 by pressing the “Standby” button (to the left of the touch panel)
  • Turn on the computer
  • Log into Windows with your Agendo credentials
  • Start the ZEN software
  • Open the Main valve of the CO2 tank (if needed) - do not change the pressure settings!

Microscope Turn Off Procedure (for complete shutdown)

Please only shutdown the system if instructed to do so by the Bioimaging Unit.

  • Close the main valve of the CO2 tank (if used)
  • Turn off the lasers on ZEN
  • Exit the ZEN software
  • Shutdown the computer
  • Press the “Standby” button (to the left of the touch panel)
  • Turn off the MAIN SWITCH (bottom right of the CD7)

Back to the Equipment page

zeiss_celldiscoverer_7.txt · Last modified: 2024/07/24 21:28 by bioimaging