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zeiss_celldiscoverer_7

Location: Room P2-A-24 ( 47219)
Manufacturer: ZEISS Microscopy
Model: Celldiscoverer 7
Nickname: "CD7"
Software: ZEN 3.5 (ZEN celldiscoverer)
Year: 2022
SN: 4648000055
 
 
 
 
 
Zeiss Celldiscoverer 7 Usage Statistics

Microscope overview

The Zeiss Celldiscoverer 7 is a fully integrated and motorized high-end inverted widefield fluorescence imaging system ideal for automated live-cell imaging in multi-well plates and Petri dishes. It offers a fully integrated incubation system with temperature and CO2 control and Definite Focus.3 for hardware focus control. The ZEN software can automatically calibrate the system and adapt if to your sample by recognizing different sample carriers, determining their bottom thickness and material and adjusting the objectives correction ring to compensate for spherical aberrations. You can use Petri dishes, chamber slides, multi-well plates, plastic or glass, thin or thick vessel bottoms, low-skirt or high-skirt plates. When using the 50x water immersion objective, automated immersion supply and removal is available for time lapse experiments over several days or extensive scanning processes on multi-well plates. The fluorescence excitation unit combines seven LEDs – from deep blue to far-red - for high-speed multi-channel imaging. Its sensitive cooled Axiocam 702 and 712 cameras allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. If you need optical sectioning for thick samples, check out one of the confocal systems such as the point scanners Zeiss LSM 880 or Zeiss LSM 980 or spinning disks 3i Marianas SDC and Zeiss Cell Observer SD. If your personal computer cannot handle all the data you collected, check out the Colossus.

Data files older than 1 month will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

System components

LEDs

Line Wavelength
UV 385 nm
V 420 nm
B 470 nm
C 520 nm
G 567 nm
Y 590 nm
R 625 nm

Filtersets

Position Filterset Reference Dichroic Emission
1 RQBS FS90HE 405 nm 410-440 nm
493 nm 499-529 nm
575 nm 580-605 nm
653 nm 659-759 nm
2 RTBS FS91HE 450 nm 455-479 nm
538 nm 543-568 nm
610 nm 615-760 nm
3 RTBS FS92HE 405 nm 410-440 nm
493 nm 499-549 nm
610 nm 616-761 nm
4 RDBS FS93HE 493 nm 498-530 nm
575 nm 580-630 nm

Objectives

Model Immersion WD (mm) NA Magnification changer Magnification
Plan-Apochromat 5x / 0.35 Air 5.10 0.12 0.5x 2.5x
0.25 1x 5x
0.35 2x 10x
Plan-Apochromat 20x / 0.7 Air 2.20 0.35 0.5x 10x
0.70 1x 20x
2x 40x
Plan-Apochromat 20x / 0.95 Air 0.76 0.50 0.5x 10x
0.80 1x 20x
0.95 2x 40x
Plan-Apochromat 50x / 1.2 W Water 0.84 1.20 0.5x 25x
1x 50x
2x 100x

Cameras

Model Type Frame Size Pixel Size (µm)
Axiocam 702 mono CMOS 1920 x 1216 5.86 x 5.86
Axiocam 712 mono CMOS CMOS 4096 x 3008 3.45 x 3.45

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zeiss_celldiscoverer_7.txt · Last modified: 2022/10/14 18:54 by bioimaging