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zeiss_lsm_880

Location: Room P2-A-22 ( 47217)
Manufacturer: Carl Zeiss MicroImaging
Model: LSM 880 with Airyscan
Nickname: "880"
Software: ZEN 2.1 (black)
Year: 2016
SN: 2811000267
 
 
 
Zeiss LSM 880 Booking
Zeiss LSM 880 Quality Control
Zeiss LSM 880 Usage Statistics

Microscope overview

airyscan.jpg The Zeiss LSM 880 with Airyscan is a confocal point-scanning microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Its Airyscan 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the Zeiss Cell Observer or the Zeiss Axiovert 200M. Image resolution with Airyscan is higher than the Zeiss LSM 710 and detection sensitivity is also higher than faster confocal systems such as the spinning disk 3i Marianas SDC or the line scanning Zeiss LSM 7 Live. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus.

Click on the image on the left to see the system beam path in higher detail

Booking Rules

The Zeiss LSM 710 booking is divided in three daily slots:

9:00 to 13:00 13:00 to 17:00 17:00 to 21:00
  1. A user can book at maximum 2 sessions per week
  2. Each session must fit inside one of the three daily slots (i.e. a session booked from 11:00 to 14:00 counts as 2 sessions)
  3. The above rules do not apply for weekends and for work days before 9:00 and after 21:00
  4. The above rules do not apply for sessions booked on the very same day or 24 hours ahead
  5. Exceptions to these rules require approval from José Rino.
  6. Users must indicate which lasers they are going to use in the booking Description field
  7. Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR)

System components

LASERs

Laser Unit Wavelength Maximum Power Current Status
Diode 405-30 405 nm 30 mW ok
Argon 458, 488 and 514 nm 25 mW ok
DPSS 561-20 561 nm 20 mW ok
HeNe594 594 nm 2 mW ok
HeNe633 633 nm 5 mW ok

Objectives

Magnification Model Type NA WD (mm)
10x EC Plan-Neofluar Dry 0.30 5.2
20x Plan-Apochromat Dry 0.80 0.55
40x C-Apochromat Water 1.20 0.28
63x Plan-Apochromat Oil 1.40 0.19

Upon request:

Magnification Model Type NA WD (mm)
25x LCI Plan-Neofluar Oil/Glyc/W 0.80 0.21
40x Plan-Apochromat Dry 0.95 0.25
100x alpha Plan-Apochromat Oil 1.46 0.11

Filtersets (Ocular)

Position Filterset Reference Excitation Emission
1 Green FS38HE 450-490 nm 500-550 nm
2 Red FS43 533-558 nm 570-640 nm
3 Blue FS49 G 365 nm 420-470 nm

Microscope Turn On Procedure

  • Turn on the MAIN SWITCH
  • Turn on the two switches: SYSTEMS/PC and COMPONENTS

zeiss_lsm_880_1.jpg

  • Turn the computer on
  • Login with your unit account
  • Wait for network icon to change to connected state
  • Start the ZEN software

Microscope Turn Off Procedure

If there's another user for this microscope in the next hour:

  • Close the software, leave the lasers on and log off the computer.
  • Clean up any objective where you used immersion oil.
  • Make sure that there really is another user going to use the microscope.

Else:

  • Turn off the lasers on ZEN
  • Clean up any objective were you used immersion oil.
  • Exit the software, shut down the computer.
  • Turn off the main switches SYSTEMS/PC and COMPONENTS
  • Wait 5 min. for Ar laser cooldown
  • Turn off the MAIN SWITCH.

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zeiss_lsm_880.txt · Last modified: 2017/10/12 11:16 by bioimaging