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zeiss_lsm_880

Location: Room P2-A-22 ( 47217)
Manufacturer: Carl Zeiss MicroImaging
Model: LSM 880 with Airyscan
Nickname: "880"
Software: ZEN 2.1 (black)
Year: 2016
SN: 2811000267
 
 
 
Zeiss LSM 880 Booking
Zeiss LSM 880 Quality Control
Zeiss LSM 880 Usage Statistics

Microscope overview

The Zeiss LSM 880 with Airyscan is a confocal point-scanning microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its Airyscan 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in Fast Mode, which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor of four, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the Zeiss Cell Observer or the Zeiss Axiovert 200M. Image resolution with Airyscan is higher than the Zeiss LSM 710 and detection sensitivity is also higher than the spinning disks 3i Marianas SDC and Zeiss Cell Observer SD. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus.

Click here to see the system beam path in higher detail

Data files older than 1 month will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

Booking Rules

The Zeiss LSM 880 booking is divided in three daily slots:

9:00 to 13:00 13:00 to 17:00 17:00 to 21:00
  1. A user can book at maximum 2 sessions per week
  2. Each session must fit inside one of the three daily slots (i.e. a session booked from 11:00 to 14:00 counts as 2 sessions)
  3. The above rules do not apply for weekends and for work days before 9:00 and after 21:00
  4. The above rules do not apply for sessions booked on the very same day or 24 hours ahead
  5. Exceptions to these rules require approval from José Rino.
  6. Users must indicate which lasers they are going to use in the booking Description field
  7. Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR)

System components

LASERs

Laser Unit Wavelength Maximum Power Current Status
Diode 405-30 405 nm 30 mW ok
Argon 458, 488 and 514 nm 25 mW ok
DPSS 561-20 561 nm 20 mW ok
HeNe594 594 nm 2 mW ok
HeNe633 633 nm 5 mW ok

Objectives

Magnification Model Immersion NA WD (mm) Reference
10x EC Plan-Neofluar Air 0.30 5.20 420340-9901-000
20x Plan-Apochromat Air 0.80 0.55 420650-9901-000
40x C-Apochromat Corr Water 1.20 0.28 421767-9970-000
63x Plan-Apochromat DIC Oil 1.40 0.19 420782-9900-799

Upon request:

Magnification Model Immersion NA WD (mm) Reference
25x LCI Plan-Neofluar Corr DIC Oil/Glyc/W 0.80 0.21 420852-9972-000
40x Plan-Apochromat Corr Dry 0.95 0.25 420660-9970-000
100x α Plan-Apochromat DIC Oil 1.46 0.11 420792-9800-000

Filtersets (Ocular)

Position Filterset Reference Excitation Emission
1 Green FS38HE 450-490 nm 500-550 nm
2 Red FS43 533-558 nm 570-640 nm
3 Blue FS49 G 365 nm 420-470 nm

Microscope Turn On Procedure

  • Turn on the MAIN SWITCH
  • Turn on the two switches: SYSTEMS/PC and COMPONENTS

zeiss_lsm_880_1.jpg

  • Turn on the computer
  • Login in to Windows (Bioimaging User)
  • Start the ZEN Black software

Microscope Turn Off Procedure

If there is another user for this microscope in the next hour:

  • Exit the ZEN software, leave the lasers on
  • Log off the computer
  • Clean up immersion objectives

Else:

  • Turn off the lasers on ZEN
  • Clean up immersion objectives
  • Exit the ZEN software
  • Shutdown the computer
  • Turn off the two switches SYSTEMS/PC and COMPONENTS
  • Wait 5 min. for Ar laser to cool down
  • Turn off the MAIN SWITCH

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zeiss_lsm_880.txt · Last modified: 2020/02/28 00:35 by bioimaging