GIMM Bioimaging Wiki

Location: [Oeiras] Room 0B03
Manufacturer: ZEISS
Model: Stereo Lumar V.12
Nickname: Lumar
Software: Micromanager (Hamamatsu camera) / uEye (uEye camera)
Year: 2011
SN:

Data will be deleted after: 3 months
→ ZEISS Stereo Lumra.V12 Quality Control
→ ZEISS Stereo Lumra.V12 Usage Statistics

Microscope overview

The Stereo Lumar V.12 is a motorized stereoscope with fluorescence, capable of imaging in colour and grayscale (camera dependent), with a zoom between 9.6x and 120x.

System components

Filtersets

Position	Filterset	Excitation	Dichroic	Emission	Reference
1	Cyan	426-446 nm	455 nm	460-500 nm	FS47
2	Green	460-490 nm	495 nm	505-530 nm	FS13
3	Red	540-580 nm	585 nm	592-667 nm	FS45
4	Yellow	490-510 nm	515 nm	520-550 nm	FS46

Camera

Camera can be changed on request, and training for this can be provided if needed.

Model	Frame Size	Pixel Size (µm)	Quantum Efficiency	Installed date
UI-3370CP Rev. 2 Color Camera	2048 × 2048	5.5 x 5.5	-	Since Oct 2024
ORCA-Flash4.0 LT+	2048 × 2048	6.5 x 6.5	82%	Before May 5, 2025
ORCA-Flash4.0 - C11440-22C	2048 × 2048	6.5 x 6.5	82%	After May 5, 2025

Pixel-size vs magnification

Magnification	Hamamatsu (µm/pixel)	IDS (µm/pixel)
9.6x	10.90	9.40
10.0x	10.49	9.02
20.0x	5.07	4.52
30.0x	3.41	3.02
40.0x	2.59	2.26
50.0x	2.05	1.81
60.0x	1.73	1.51
70.0x	1.49	1.30
80.0x	1.29	1.13
90.0x	1.16	1.01
100.0x	1.04	0.91
110.0x	0.95	0.83
120.0x	0.87	0.76

Microscope Turn On Procedure

Turn on the powerbar below the stone table.
If fluorescence is necessary, turn on the fluorescent lamp controller on top of the desk.

Microscope Turn Off Procedure

Turn on the powerbar below the stone table.
If fluorescence was used, turn off the fluorescent lamp controller on top of the desk.
- This lamp has to be on for 30 minutes. If you use the system less than 30 minutes, wait until that time to turn the lamp off, or warn the Bioimaging Team.

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