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zeiss_lsm_900

Location: [Oeiras] Room 0B03
Manufacturer: ZEISS Microscopy
Model: LSM 900 with Airyscan 2
Nickname: "900"
Software: Zen 3.5
Year: 2022
SN:
 
Data will be deleted after: 1 month  
 
ZEISS LSM 900 with Airyscan 2 Quality Control
ZEISS LSM 900 with Airyscan 2 Usage Statistics

Microscope overview

The Zeiss LSM 900 with Airyscan 2 is a point scanning confocal microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a dark large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus.3 for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions, using the Tiles Advanced Setup of ZEN blue. Its Airyscan 2 with 32 concentrically arranged GaAsP detection elements is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (120 nm laterally and 350 nm axially in SR mode, at 488 nm). It can also be used in Multiplex Mode, which enables parallel excitation and detection in 4Y modes. The result is a speed improvement by a factor of 4x, while maintaining the sensitivity of Airyscan. Its scanning unit further includes two PMT detectors as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 488, 561 and 639 nm excitation wavelengths). The LSM Plus deconvolution mode enhances signal-to-noise ratio by acting as a denoising step post image acquisition, allowing for high acquisition speed and low laser power. With Airyscan Joint Deconvolution (jDCV) the resolution can be improved to 90 nm (XY) and 270 nm (Z).

Data files older than 1 month will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.

System components

LASERs

Laser Unit Wavelength Maximum Power
405 405 nm 15 mW
488 488 nm 13 mW
561 561 nm 13 mW
640 640 nm 10 mW

LEDs (Ocular)

Line Wavelength
UV 385 nm
B 475 nm
G 555 nm
R 630 nm

Objectives

Magnification Model Immersion NA WD (mm) Reference
5x EC Plan-Neofluar Air 0.16 18.5 420330-9901-000
10x Plan-Apochromat Air 0.45 2 420640-9900-000
20x Plan-Apochromat Air 0.80 0.55 420650-9903-000
40x LD LCI Plan-Apochromat W, Sil, G 1.2 0.41 420862-9970-799
63x Plan-Apochromat DIC Oil 1.40 0.19 420782-9900-799

Emission Filters

Filter name Transmission(nm) Reference
BFP 450/40 96 HE
GFP 425/50 38 HE
Alexa 546 LP590 15
Cy5 690/50 50
DIC <800 -
mPlum 629/62 64 HE
Quad 425/514/592/709 90 HE

Microscope Turn On Procedure

  • Turn on the Laser Key

  • Turn on the System and Components switch

  • Turn on the Power bar

  • Turn on the computer
  • Log into Windows with your Agendo credentials
  • Start the ZEN software

Microscope Turn Off Procedure (for complete shutdown)

Only shutdown te system if there is no one else booked for the next two hours

  • Exit the ZEN software
  • Shutdown the computer
  • Turn off the Power Bar
  • Turn off the System and Components switch
  • Turn off the Laser Key

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zeiss_lsm_900.txt · Last modified: 2025/01/13 16:21 by bioimaging