The Flow Cytometry Facility provides a state of the art flow cytometry service to iMM researchers as well as external groups from other research institutes.
We aim at training researchers in the adequate use of the instruments and also on flow cytometry principles, experiment planning, as well as data analysis and interpretation.
The Flow Cytometry Facility also provides a cell sorting service using two High Speed Cell Sorters (BD FACSAria III and BD FACSAria IIu) operated by dedicated staff.
More Information:Flow Cytometry Wiki
Instruments and Services
Our flow cytometers are grouped into two categories: analyzers and cell sorters. Analyzers are instruments for acquisition only, cells cannot be recovered once they are analyzed in the instrument. Cell sorters are capable of physically “sorting” out a population(s) of interest.
Available for booking from 10am to 10pm from Monday to Friday.
The cell purification service is provided by a dedicated technician upon reservation on the online booking system.
Available 7 days a week, 24 hours a day for use by trained researchers upon reservation on the online booking system.
- BD LSRFortessa 1 (user's guide)
- BD LSRFortessa 2 (user's guide) equipped with a High Throughput Sampler (HTS) which allows the automated acquisition of samples from 96 and 384 well plates.
- BD LSRFortessa X-20 (user's guide)
- BD Accuri C6 (user's guide)
Imaging Flow Cytometer
- Amnis ImageStreamX - benchtop multispectral imaging flow cytometerequipped with two CCD cameras that can acquire up to 12 images simultaneously of each cell or object
Data analysis can be performed on a dedicated Mac workstation with FlowJo, ModFit, FACSDIVA and CellQuest software. No training is required to use the computer. This service is free of charge, however pre-reservation is required on the online booking system.
Training and Support
The flow cytometry service runs seminar series targeted at new users that combine lectures on basic flow cytometry concepts and research applications with practical hands-on tutorials.
How to use the Service
All new users must contact the Flow Cytometry Facility to get proper training in using the systems and know the usage rules. Only after training will a user gain access to the Flow Cytometry room and to the online booking system.
To get access to the cell sorters online booking, users must contact the Flow Cytometry staff.
Please read our policies and guidelines here.
Users are billed for the actual time of usage.