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imagestreamx

Location: Room P2-A-49 ( 47224)
Manufacturer: Merck
Model: Amnis ImageStreamX Mark II
Nickname: "ImageStream"
Software: INSPIRE 200.1.620.0 / IDEAS 6.2.64.0
Year: 2017
SN: ISX540
 
 
 
 
Amnis ImageStreamX Booking
Amnis ImageStreamX Usage Statistics  

System overview

The Amnis ImageStreamX is a benchtop multispectral imaging flow cytometer equipped with two CCD cameras that can acquire up to 12 images simultaneously of each cell or object in brightfield, scatter and multiple fluorescent channels at rates up to 5000 objects/sec. It has five lasers - 375 nm, 488 nm, 561 nm, 642 nm and 785 nm (dedicated to side scatter). Cells in suspension are hydrodynamically focused in a flow cell, where they are illuminated by a brightfield light source and by lasers. High numerical aperture (NA) objective lenses collect fluorescence emission, as well as scattered and transmitted light from the cells which then intersect a spectral decomposition element that focus different spectral bands on different physical locations of two CCD cameras. By collecting large numbers of digital images per sample and providing numerical representation of image-based features, the ImageStreamX combines single cell standard microscopy information with the statistical significance of large sample sizes in standard flow cytometry. While you can use it to measure fluorescence intensity as in conventional flow cytometers, the best applications for the ImageStreamX take advantage of its imaging abilities to locate and quantify the distribution of signals within cells or even between cells in cell conjugates. The system also has a dedicated software application used for spectral compensation, image analysis and statistical analysis of acquired images (IDEAS) with predefined wizards for apoptosis, cell cycle, co-localization, internalization, shape change, spot counting and others.

  • 12 imaging channels (2 brightfield channels + 10 channels for fluorescence detection)
  • 5 lasers with UV (375 nm), blue (488 nm), yellow-green (561 nm), red (642 nm) and far red (785 nm) for side scatter
  • Multimag: 20x, 40x and 60x magnification
  • Extended depth of field: projects all structures within a cell into a single plane of focus

Configuration

Objectives Information

Magnification 20x 40x 60x
Numerical Aperture 0.50 0.75 0.90
Field of View (μm) 120 60 40
Pixel Size (μm) 1.0 0.5 0.33
Depth of Field (μm) 8.0 4.0 2.5

Image Acquisition Performance

Additional information

Booking Rules

Available 7 days a week, 24 hours a day for trained researchers.

Please check the User Guidelines for detailed information.

Cleaning Protocol

Are you done? Just click on Shutdown and select Shudown after sterilize. The ImageStreamX will automatically turn off all illumination sources and rinse the entire fluidic system with water, sterilizer, cleanser, de-bubbler, and then water again. The sterilizer is held in the system for ten minutes to ensure decontamination.

You can walk away from the system. It takes about 45 minutes of unattended operation to complete.


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imagestreamx.txt · Last modified: 2018/06/11 15:15 by flowcytometry