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bd_lsr_fortessa_2

Location: Room P2-A-49 ( 47224)
Manufacturer: BD Biosciences
Model: LSRFortessa
Nickname: "Fortessa 2"
Software: FACSDiva 6.2
Year: 2010
SN: H5B300009
 
 
 
 
BD LSRFortessa 2 Booking
BD LSRFortessa 2 Usage Statistics  

Next Scheduled Maintenances: 16 June and 15 December, 2021

System overview

The BD LSRFortessa 2 cell analyzer is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has three spatially separated lasers - 405 nm, 488 nm and 640 nm. Its primary function is to characterize complex populations of cells. Individual cells pass single file through a stream, which is interrogated by the three lasers with the emitted light being detected by photomultiplier (PMT) tubes. The system electronics calculates the time it takes a single cell to pass through each laser intercept and assigns the signal of each PMT to the appropriate cell. Besides fluorescence, the physical properties of the cell are also analyzed with side scatter (SSC), which reflects the internal complexity of a cell and forward scatter (FSC), which is roughly proportional to cell size. The sample flow rate can be set to low, medium and high values, which correspond approximately to 12, 35 and 60 μl/minute, respectively. The LSRFortessa 2 is equipped with a High Throughput Sampler (HTS), which allows the automated acquisition of samples from 96 and 384 well plates. If your cells are stained with PerCP, check the BD LSRFortessa 1 instead. If you need multicolor analysis of up to 16 parameters, check the BD LSRFortessa X-20.

  • Three lasers with violet (405 nm), blue (488 nm) and red (640 nm)
  • Multicolor analysis of up to 10 parameters

Configuration

Fluorophores Compatibility






Additional Information

Booking Rules

Available 7 days a week, 24 hours a day for trained researchers.

Please check the User Guidelines for detailed information.


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bd_lsr_fortessa_2.txt · Last modified: 2021/04/06 11:28 by flowcytometry