Edgar Gomes Lab

Arquitetura Celular

Ligar o núcleo ao citoesqueleto é relevante para vários processos celulares e as perturbações destas ligações resultam em várias patologias. O posicionamento nuclear dentro do citoplasma da célula requer uma conexão entre o núcleo e o citoesqueleto.

No nosso laboratorio estamos interessados em compreender os processos envolvidos nestas ligações e o papel do posicionamento do núcleo na função da célula.

No nosso laboratório estudamos a migração celular e formação de células musculares esqueléticas que exigem a ligação entre o núcleo e o citoesqueleto e o posicionamento preciso do núcleo. Usamos diferentes abordagens moleculares e celulares em  combinação com a análise em video para responder a estas perguntas.

Equipa de Investigação

Áreas de Investigação

• Mecanismos de movimento do núcleo durante a migração celular e formação de músculo esquelético
• Função do posicionamento do núcleo na formação do músculo esquelético

Projetos de Investigação em Curso

2019-2025 ERC Synergy

2018-2022 FETOPEN - MyoChip Project

2018-2021 FCT LISBOA-01-0145-FEDER-031075

2018-2020 AFM-Telethon

Projetos de Investigação Anteriores

2016-2018 ERC proof of concept Grant

2016-2019 Coordinator of H2020-TWINN-2015 Cancer Biology consortium

2014-2019 ERC Consolidator Grant

2014-2019 EMBO Installation Grant

2014-2019 Investigador FCT

Publicações Selecionadas

• Infante E, Castagnino A, Ferrari R, Monteiro P, Agüera-González S, Paul-Gilloteaux P, Domingues MJ, Maiuri P, Raab M, Shanahan CM, Baffet A, Piel M, Gomes ER, Chavrier P.LINC complex-Lis1 interplay controls MT1-MMP matrix digest-on-demand response for confined tumor cell migration.  Nat Commun. 2018 Jun 22;9(1):2443. doi: 10.1038/s41467-018-04865-7
• Roman W, Martins JP, Gomes ER. Local Arrangement of Fibronectin by Myofibroblasts Governs Peripheral Nuclear Positioning in Muscle Cells. Dev Cell. 2018 Jun 15. pii: S1534-5807(18)30430-1. doi: 10.1016/j.devcel.2018.05.031.
• Calero-Cuenca FJ, Janota CS, Gomes ER. Dealing with the nucleus during cell migration. Curr Opin Cell Biol. 2018 Feb;50:35-41. doi: 10.1016/j.ceb.2018.01.014.
• Roman W, Gomes ER. Nuclear positioning in skeletal muscle. Semin Cell Dev Biol. 2017 Dec 11. pii: S1084-9521(17)30428-7. doi: 10.1016/j.semcdb.2017.11.005.
• Gimpel, P., Lee, Y.L., Sobota, R.M., Calvi, A., Koullourou, V., Patel, R., Mamchaoui, K., Nédélec, F., Shackleton, S., Schmoranzer, J., Burke, B., Cadot, B., Gomes, E. R.(2017) Nesprin-1α-Dependent Microtubule Nucleation from the Nuclear Envelope via Akap450 Is Necessary for Nuclear Positioning in Muscle Cells. Current Biology. doi.org/10.1016/j.cub.2017.08.031
• Roman, W., Martins, J., Carvalho, F. A., Voituriez, R., Abella, J. V. G., Santos, N. C., Cadot, B., Way, M., and Gomes, E. R.(2017) Myofibril contraction and crosslinking drive nuclear movement to the periphery of skeletal muscle. Nat. Cell Biol. 1189–1201 doi:10.1038/ncb3605
• Cátia S. Janota, Francisco J. Calero-Cuenca, Judite Costa, Edgar R. Gomes (2017). SnapShot: Nucleo-cytoskeletal Interactions. Cell 2017; 169(5):970-970.e1.
• Pimentel MR, Falcone S, Cadot B, Gomes ER. In Vitro Differentiation of Mature Myofibers for Live Imaging. J Vis Exp. 2017 Jan 7;(119). doi: 10.3791/55141.
• Vilmont V, Cadot B, Vezin E, Le Grand F, Gomes ER. Dynein disruption perturbs post-synaptic components and contributes to impaired MuSK clustering at the NMJ: implication in ALS. Sci Rep. 2016 Jun 10;6:27804. doi: 10.1038/srep27804.
• Vilmont V, Cadot B, Ouanounou G, Gomes ER. A system for studying mechanisms of neuromuscular junction development and maintenance. Development. 2016 Jul 1;143(13):2464-77. doi: 10.1242/dev.130278. Epub 2016 May 25.
• D’Alessandro, M., Hnia, K., Gache, V., Koch, C., Gavriilidis, C., Rodriguez, D., Nicot, A.-S., Romero, N.B., Schwab, Y., Gomes, E.R., Labouesse, M., Laporte, J. (2015). Amphiphysin 2 Orchestrates Nucleus Positioning and Shape by Linking the Nuclear Envelope to the Actin and Microtubule Cytoskeleton. Developmental Cell 35, 186–198.
• Cadot B, Gache V, Gomes ER. Moving and positioning the nucleus in skeletal muscle - one step at a time. Nucleus. 2015;6(5):373-81. doi: 10.1080/19491034.2015.1090073.
• Sestina Falcone, William Roman, Karim Hnia, Vincent Gache, Nathalie Didier, Jeanne Lainé, Frederic Aurade, Isabelle Marty, Ichizo Nishino, Nicolas Charlet-Berguerand, Norma Romero, Giovanna Marazzi, David Sassoon, Jocelyn Laporte, Edgar R. Gomes. N-WASP is required for Amphiphysin-2/BIN1-dependent nuclear positioning and triad organization in skeletal muscle and is involved in the pathophysiology of centronuclear myopathy. EMBO Mol Med 6, 1455–1475 (2014) (PDF)
• Cadot B, Gache V, Gomes ER. Fast, multi-dimensional and simultaneous kymograph-like particle dynamics (SkyPad) analysis. PLoS One. 2014 Feb 19;9(2):e89073. doi: 10.1371/journal.pone.0089073.
• Osorio DS, Gomes ER. Connecting the nucleus to the cytoskeleton for nuclear positioning and cell migration. Adv Exp Med Biol. 2014;773:505-20. doi: 10.1007/978-1-4899-8032-8_23.

• Osorio DS, Gomes ER. The contemporary nucleus: a trip down memory lane. Biol Cell. 2013 Sep;105(9):430-41. doi: 10.1111/boc.201300009.
• Gache V, Cadot B, Gomes ER. [Spatial distribution of nuclei in muscle fibers: a novel actor of muscular function]. Med Sci (Paris). 2012 Jun-Jul;28(6-7):577-9. doi: 10.1051/medsci/2012286007.

• Metzger, T., Gache, V., Xu, M., Cadot, B., Folker, E., Richardson, B., Gomes, E.R.*, Baylies, M.K.*. MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function. Nature 484, 120–124 (2012), *co-last and corresponding author
• Cadot, B., Gache, V., Vazyutina, E., Falcone, S., Birchmeier, C., Gomes. E.R. Nuclear movement during myotube formation is microtubule and dynein dependent and is regulated by Cdc42, Par6 and Par3. EMBO Reports, 13(8):741-9 (2012)
• Borrego-Pinto, J., Jegou, T., Osorio, D., Aurade, F., Gorjánácz, M., Koch, B., Mattaj, I., and Gomes, E. R. (2011). Samp1 is a component of TAN lines and is required for nuclear movement. Journal of Cell Science, 125, 1099–105 (2012)
• Luxton GW, Gomes ER, Folker ES, Worman HJ, Gundersen GG. TAN lines: a novel nuclear envelope structure involved in nuclear positioning. Nucleus. 2011 May-Jun;2(3):173-81. doi: 10.1073/pnas.1000824108.
• Luxton GW*, Gomes ER*, Folker ES, Vintinner E, Gundersen GG. Linear arrays of nuclear envelope proteins harness retrograde actin flow for nuclear movement. Science. 2010 Aug 20;329(5994):956-9.*co-first author
• Mitchell KJ, Pannérec A, Cadot B, Parlakian A, Besson V, Gomes ER, Marazzi G, Sassoon DA. Identification and characterization of a non-satellite cell muscle resident progenitor during postnatal development. Nat Cell Biol. 2010 Mar;12(3):257-66. doi: 10.1038/ncb2025.
• Mitchell KJ, Pannérec A, Cadot B, Parlakian A, Besson V, Gomes ER, Marazzi G, Sassoon DA. Identification and characterization of a non-satellite cell muscle resident progenitor during postnatal development. Nat Cell Biol. 2010 Mar;12(3):257-66. doi: 10.1038/ncb2025.
• Ostlund C, Folker ES, Choi JC, Gomes ER, Gundersen GG, Worman HJ. Dynamics and molecular interactions of linker of nucleoskeleton and cytoskeleton (LINC) complex proteins. J Cell Sci. 2009 Nov 15;122(Pt 22):4099-108. doi: 10.1242/jcs.057075.
• Gomes ER, Gundersen GG. Real-time centrosome reorientation during fibroblast migration. Methods Enzymol. 2006;406:579-92
• E.R. Gomes, S. Jani, G.G. Gundersen. Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells. Cell, 2005, 121: 451-63
• Evans KJ, Gomes ER, Reisenweber SM, Gundersen GG, Lauring BP. Linking axonal degeneration to microtubule remodeling by Spastin-mediated microtubule severing. J Cell Biol. 2005 Feb 14;168(4):599-606.
• Gundersen GG, Gomes ER, Wen Y. Cortical control of microtubule stability and polarization. Curr Opin Cell Biol. 2004 Feb;16(1):106-12
• Dujardin DL, Barnhart LE, Stehman SA, Gomes ER, Gundersen GG, Vallee RB. A role for cytoplasmic dynein and LIS1 in directed cell movement. J Cell Biol. 2003 Dec 22;163(6):1205-11

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