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Neuronal Communication & Synaptopathies

Neurological and neuropsychiatric diseases constitute an overwhelming social and economic load, with predicted exponential increase in the near future due to ageing, drug abuse, traumatic life styles. There is growing awareness that a common feature in these diseases is a dysfunction of neuronal communication, that is to say, a synaptic dysfunction. The major focus of the Unit is to evaluate how synapses are modulated and how these processes are altered by disease states or reverted by putative therapeutics.

Under specific focus at the moment are synaptic dysfunction in developmental epilepsy pathologies such as Rett syndrome, synaptic circuitry remodeling after stroke and seizures, synaptic reorganization after chronic drug abuse, functional alterations of synaptic function at the onset of neurodegenerative diseases such as amyotrophic lateral sclerosis and Alzheimer’s disease.

Research Team

Adam Armada-Moreira
PhD Student
adam.moreira@medicina.ulisboa.pt

Alexandra Botelho
Administrative Technician
balexandra@medicina.ulisboa.pt

Alexandre Ribeiro
Staff Scientist
jaribeiro@medicina.ulisboa.pt

Ana Gomes
PhD Student
anarita.gomes@medicina.ulisboa.pt

Anwesha Ghosh
PhD Student
anwesha.ghosh@medicina.ulisboa.pt

Armando Cruz
PhD Student
adcruz@medicina.ulisboa.pt

Carolina Borlido
MSc Student
cborlido@medicina.ulisboa.pt

Carolina Pina
MSc Student
carolinacampospina@medicina.ulisboa.pt

Catarina Ferreira
PhD Student
catarina.ferreira@medicina.ulisboa.pt

Catarina Lourenço
PhD Student
catarinalourenco@medicina.ulisboa.pt

Celine Freitas
MSc Student
celine.freitas@medicina.ulisboa.pt

Cláudia Valente
Senior Postdoctoral Researcher
cvalentecastro@medicina.ulisboa.pt

Cristina Varandas
Administrative Technician
cvarandas@medicina.ulisboa.pt

Daniela Magalhães
PhD Student
dmagalhaes@medicina.ulisboa.pt

Diogo Dias
MSc Student
diogo.dias@medicina.ulisboa.pt

Diogo Lourenço
PhD Student
diogo.lourenco@medicina.ulisboa.pt

Diogo Rombo
Postdoctoral Researcher
drombo@medicina.ulisboa.pt

Filipa F. Ribeiro
Postdoctoral Researcher
anaribeiro@medicina.ulisboa.pt

Francisco Meda
MSc Student
fmeda@medicina.ulisboa.pt

Joana Gomes
MSc Student
joana.gomes@medicina.ulisboa.pt

Joana Mateus
PhD Student
joana.mateus@medicina.ulisboa.pt

Joana Ribeiro
PhD Student
jgribeiro@medicina.ulisboa.pt

Jorge Ferreira
Trainee
jorge.ferreira@medicina.ulisboa.pt

João Gomes
PhD Student
joaogomes@medicina.ulisboa.pt

João Moreira
Trainee
joaomoreira@medicina.ulisboa.pt

Leonor Ribeiro-Rodrigues
Lab Technician
leonorrodrigues@medicina.ulisboa.pt

Mafalda Carvalho
MSc Student
mafaldacarvalho@medicina.ulisboa.pt

Mafalda Manso
MSc Student
mafalda.manso@medicina.ulisboa.pt

Maria José Diógenes
Staff Scientist
diogenes@medicina.ulisboa.pt

Mariana Campos
MSc Student
mariana.campos@medicina.ulisboa.pt

Miranda Mele
Postdoctoral Researcher
miranda.mele@medicina.ulisboa.pt

Nádia Rei
PhD Student
nadiarei@medicina.ulisboa.pt

Oksana Savchak
MSc Student
osavchak@medicina.ulisboa.pt

Pedro Dias
MSc Student
pdias@medicina.ulisboa.pt

Rita A. Soares
PhD Student
ritasoares@medicina.ulisboa.pt

Rita Belo
PhD Student
rbelo@medicina.ulisboa.pt

Rui Rodrigues
PhD Student
rmsrodrigues@medicina.ulisboa.pt

Sandra Vaz
Senior Postdoctoral Researcher
svaz@medicina.ulisboa.pt

Sara Paulo
PhD Student
sara.paulo@medicina.ulisboa.pt

Sara Pinto
PhD Student
sara.pinto@medicina.ulisboa.pt

Sara Tanqueiro
PhD Student
saratanqueiro@medicina.ulisboa.pt

Sara Xapelli
Staff Scientist
sxapelli@medicina.ulisboa.pt

Sodikdion A. Kodirov
Postdoctoral Researcher
skodirov@medicina.ulisboa.pt

Svitlana Zavalko
MSc Student
szavalko@medicina.ulisboa.pt

Tatiana Morais
PhD Student
tpmorais@medicina.ulisboa.pt

Tiago Costa Coelho
MSc Student
tcoelho@medicina.ulisboa.pt

Research Areas

The Unit is constituted by balanced multidisciplinary team organized in five groups according to their specific research topics, as follows:

  • Modulation of the tripartite synapse. This group aims to elucidate pre- post and glial mechanisms involved in the control of the delicate balance between neuronal inhibition and excitation and its implications for synaptic plasticity. Following this research line, the group evaluates the synaptic and behavioural (learning, memory, anxiety, depression) consequences of long term manipulation of endogenous modulator (adenosine and cannabinoids) signaling.
  • Motor neuron. This group aims to elucidate the fine tune control of motor neuron activity under normal and pathological conditions, as amyotrophic lateral sclerosis (ALS).
  • Neurotrophic factors. This group aims to understand the causes and mechanisms of neuronal dysfunction caused by the lack of trophic support to synapse formation/ maturation/ protection. The implications for developmental epilepsies, ageing and Alzheimer’s disease are under focus.
  • Neurobiology of inflammation - interplay with epilepsy. This group aims to understand the relationship between inflammation and epilepsy to identify key points in the inflammatory cascade that may constitute as therapeutic targets to prevent epileptogenesis.
  • Control of post-natal neurogenesis and oligodendrogenesis. The group aims to understand how post-natal formation of new neurons and oligodendrocytes is regulated and its implications for the control of neuronal diseases as multiple sclerosis.

Ongoing Research Projects

2018/2021 Restabelecimento dos níveis de Adenosina: nova abordagem para o tratamento da Sindrome de Rett. Coordinator: Maria José Diógenes. Funding Agency: Fundação para a Ciência e a Tecnologia.

2018/2021 Astrossomas: Astrócitos Artificiais como reguladores da comunicação neuronal. Coordinator: Sandra Vaz. Funding Agency: Fundação para a Ciência e a Tecnologia.

2018/2021 Na procura do mecanismo de ação de um novo e seletivo fármaco antiepiléptico. Coordinator: Coordinator: Ana M Sebastião. Funding Agency: Fundação para a Ciência e a Tecnologia.

2018/2021 Regulação da homeostase e polarização pró-inflamatória da microglia Rac1. Coordinator: Ana M. Sebastião. Funding Agency: Fundação para a Ciência e a Tecnologia.

2018/2021 Titanic- CYP46A1 as new Therapeutic Target in Niemann-Pick Type-C disease. Coordinator: Maria José Diógenes. Funding Agency: Fundação para a Ciência e a Tecnologia.

2018/2021 Controlo mecanistico e optogenético da astroglia para reparação neuronal. Coordinator: Ana M. Sebastião. Funding Agency: Fundação para a Ciência e a Tecnologia.

2018/2020 Novel therapeutic strategy and new biomarker for Alzheimer´s Disease based on BDNF receptor cleavage. Coordinator: Maria José Diógenes. Funding Agency: Santa Casa da Misericórdia de Lisboa.

Selected Publications

  1. Magalhães DM, Pereira N, Rombo DM, Beltrão-Cavacas C, Sebastião AM, Valente CA (2018). Ex vivo model of epilepsy in organotypic slices-a new tool for drug screening. Journal of Neuroinflammation 15:203.
  2. Mouro FM, Batalha VL, Ferreira DG, Coelho JE, Baqi Y, Müller CE, Lopes LV, Ribeiro JA, Sebastião AM (2017). Chronic and acute adenosine A2A receptor blockade prevents long-term episodic memory disruption caused by acute cannabinoid CB1 receptor activation. Neuropharmacology 117:316-327.
  3. Ribeiro FF, Xapelli S, Miranda-Lourenço C, Tanqueiro SR, Fonseca-Gomes J, Diógenes MJ, Ribeiro JA, Sebastião AM (2016). Purine nucleosides in neuroregeneration and neuroprotection, Neuropharmacology 104:226-242.
  1. Ribeiro FF, RNeves-Tomé R, Assaife-Lopes N, Santos TE, Silva RFM, Brites D, Ribeiro JA, Sousa MM, Sebastião AM (2015) Axonal elongation and dendritic branching is enhanced by adenosine A2A receptors activation in cerebral cortical neurons. Brain Struct & Func (in the press).
  2. Nascimento F, Sebastião AM, Ribeiro JA. (2015) Presymptomatic and symptomatic ALS SOD1(G93A) mice differ in adenosine A1 and A 2A receptor-mediated tonic modulation of neuromuscular transmission. Purinergic Signal. (in the press)
  3. Jerónimo-Santos A, Fonseca-Gomes J, Guimarães DA, Tanqueiro SR, Ramalho RM, Ribeiro JA, Sebastião AM, Diógenes MJ (2015) Brain-derived neurotrophic factor mediates neuroprotection against Aβ-induced toxicity through a mechanism independent on adenosine 2A receptor activation. Growth Factors 12:1-11.
  4. Aroeira RI, Sebastião AM and Valente CA (2015). BDNF, via truncated TrkB receptor, modulates GlyT1 and GlyT2 in astrocytes. Glia. 63(12):2181-97.
  5. Rombo DM, Dias RB, Duarte ST, Ribeiro JA, Lamsa KP, Sebastião AM (2015). Adenosine A1 receptor suppresses Tonic GABAA receptor currents in hippocampal pyramidal cells and in a defined subpopulation of interneurons. Cereb Cortex. 25(9):3107-21.
  6. Pousinha PA, Correia AM, Sebastião AM, Ribeiro JA (2015) The giant miniature endplate potentials frequency is increased in aged rats. Neurosci Lett. 584C:224-229 (ePub October 2014).
  7. Félix-Oliveira A, Dias RB, Colino-Oliveira M, Rombo DM, Sebastião AM (2014) Homeostatic Plasticity induced by Brief Activity Deprivation Enhances Long-Term Potentiation in the Mature Rat Hippocampus. J Neurophysiol 112:3012-1022.
  8. Jacob JP, Vaz SH, Ribeiro JA, Sebastião AM (2014) P2Y1 receptor inhibits GABA transport through a calcium signalling-dependent mechanism in rat cortical astrocytes. Glia 62:1211-1226.
  9. Diógenes MJ, Neves-Tomé R, Fucile S, Martinello K, Scianni M, Theofilas P, Lopatár J, Ribeiro JA, Maggi L, Frenguelli BG, Limatola C, Boison D, Sebastião AM (2014). Homeostatic Control of Synaptic Activity by Endogenous Adenosine is Mediated by Adenosine Kinase. Cereb Cortex.24:67-80.
  10. Jerónimo-Santos A, Vaz SH, Parreira S, Rapaz-Lérias S, Caetano AP, Buée-Scherrer V, Castrén E, Valente CA, Blum D, Sebastião AM, Diógenes MJ (2014) Dysregulation of TrkB Receptors and BDNF Function by Amyloid-β Peptide is Mediated by Calpain. Cereb Cortex 25(9):3107-21.
  11. Rodrigues TM, Jerónimo-Santos A, Sebastião AM, Diógenes MJ (2014) Adenosine A2A Receptors as novel upstream regulators of BDNF-mediated attenuation of hippocampal Long-Term Depression (LTD). Neuropharmacology 79:389-398.
  12. Assaife-LopesN, Sousa VC, Pereira DB, RibeiroJA, Sebastião AM (2014) Regulation of TrkB receptor translocation to lipid rafts by adenosine A2A receptors and its functional implications for BDNF-induced regulation of synaptic plasticity. Purinergic Signalling 10:251-267
  13. Jerónimo-Santos A, Batalha VL, Müller CE, Baqi Y, Sebastião AM, Lopes LV, Diógenes MJ. (2014) Impact of in vivo chronic blockade of adenosine A2A receptors on the BDNF-mediated facilitation of LTP. Neuropharmacology 83:99-106.
  14. Pires M, Raischel F, Vaz SH, Cruz-Silva A, Sebastião AM, Lind PG (2014) Modeling the functional network of primary intercellular Ca2+ wave propagation in astrocytes and its application to study drug effects, Journal of Theoretical Biology 356C:201-212.
  15. Nascimento, F, Pousinha PA, Marçal-Correia A; Gomes R, Sebastião AM, Ribeiro JA (2014) "Adenosine A2A receptors activation facilitates neuromuscular transmission in the pre-symptomatic phase of the SOD1(G93A) ALS mice, but not in the symptomatic phase". PLoS ONE 9(8):e104081
  16. Rocha, MC, Pousinha PA, Correia AM, Sebastião AM, Ribeiro JA (2013) Early changes of neuromuscular transmission in the SOD1(G93A) mice model of ALS start long before motor symptoms onset. PLOS ONE, Sep 5;8(9):e73846. doi: 10.1371/journal.pone.0073846. eCollection
  17. Aroeira RI, Sebastião AM, Valente CA (2014) GlyT1 and GlyT2 in brain astrocytes: expression, distribution and function. Brain Struct Funct 219:817-30.
  18. Dias RB, Rombo DM, Ribeiro JA, Henley JM, Sebastião AM (2013) Adenosine: setting the stage for plasticity. Trends in Neuroscience 36:248-257.
  19. Cristóvão-Ferreira S, Navarro G, Brugarolas M, Pérez-Capote K, Vaz SH, Fattorini G, Conti F, Lluis C, Ribeiro JA, Mccormick PJ, Casadó V, Franco R, Sebastião AM (2013) A1R-A2AR heteromers coupled to Gs and Gi/0 proteins modulate GABA transport into astrocytes. Purinergic Signalling 9:433-449
  20. Dias RB, Rombo DM, Ribeiro JA, Sebastião AM (2013) Ischemia-induced synaptic plasticity drives sustained expression of calcium-permeable AMPA receptors in the hippocampus. Neuropharmacology 65:114-122.
  21. Pousinha PA, Correia AM, Sebastião AM and Ribeiro JA. (2012). Neuromuscular transmission modulation by adenosine upon aging. Neurobiol Aging33: 2869-2880.
  22. Dias RB, Ribeiro JA, Sebastião AM (2012). Enhancement of AMPA currents and GluR1 membrane expression through PKA- coupled adenosine A2A receptors. Hippocampus 22: 276-291.
  23. Vaz SH, Jorgensen TN, Cristovão-Ferreira S, Duflot S, Ribeiro JA, Gether U, Sebastião AM (2011) Brain-derived neurotrophic factor (BDNF) enhances GABA transport by modulating the trafficking of GABA transporter-1 (GAT-1) from the plasma membrane of rat cortical astrocytes. J Biol Chem 286:40464-4076.
  24. Sousa VC, Assaife-Lopes N, Ribeiro JA, Pratt JA, Brett RR, Sebastião AM (2011) Regulation of hippocampal cannabinoid CB1 receptor actions by adenosine A1 receptors and chronic caffeine administration: implications for the effects of ∆9-tetrahydrocannabinol on spatial memory. Neuropsychopharmacology 36, 472-487.
  25. Moreno* E, Vaz* SH, Cai NS, Ferrada C, Quiroz C, Barodia SK, Kabbani N, Canela EI, McCormick PJ, Luis C, Franco R, Ribeiro JA, Sebastião AM*, Ferré S* (2011) Dopamine-Galanin Receptor Heteromers Modulate Cholinergic Neurotransmission in the Rat Ventral Hippocampus. J Neurosci. 31:7412-7423. *Co-first authors and co-senior authors
  26. Diógenes MJ, Costenla AR, Lopes LV, Jerónimo-Santos A, Sousa VC, Fontinha BM, Ribeiro JA, Sebastião AM (2011) Enhancement of LTP in aged rats is dependent on endogenous BDNF. Neuropsychopharmacology 36:1823-1836.
  27. Kemppainen S, Rantamäki T, Jerónimo-Santos A, Lavasseurd G, Autio H, Karpova N, Kärkkäinena E, Stavéna S, Miranda HV, Outeiro TF, Diógenes MJ, Laroched S, Davis S, Sebastião AM, Castrén E, Tanila H (2011) Impaired TrkB receptor signaling contributes to memory impairment in APP/PS1 mice. Neurobiol Aging. 33(6):1122.e23-39.
  28. Aroeira RI, Ribeiro JA, Sebastião AM, Valente CA. (2011) Age-related changes of glycine receptor at the rat hippocampus: from the embryo to the adult. J Neurochem. 118:339-53
  29. Fernandes CC, Pinto-Duarte A, Ribeiro JA, Sebastião AM (2008) Postsynaptic action of brain-derived neurotrophic factor attenuates alpha7 nicotinic acetylcholine receptor-mediated  responses in  hippocampal interneurons. J Neurosci. 28:5611-5618
  30. Diógenes MJ, Fernandes CC, Sebastião AM, Ribeiro JÁ (2004) Activation of adenosine A2A receptor facilitates BDNF modulation of synaptic transmission in hippocampal slices. J. Neurosci. 24: 2905-2913.
group leader :
Ana Sebastião