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Isothermal Titration Calorimeter

Isothermal Titration Calorimetry (ITC) is used for measuring biomolecular interactions. ITC simultaneously determines all binding parameters (n, K, ∆H and ΔS) in a single experiment – information that cannot be obtained from any other method. When substances bind, heat is either generated or absorbed. ITC is a thermodynamic technique that directly measures the heat released or absorbed during a biomolecular binding event. Measurement of this heat allows accurate determination of binding constants (KB), reaction stoichiometry (n), enthalpy (∆H) and entropy (ΔS), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment.Interactions between any two molecules can be studied with ITC, including:
  • Protein-small molecule
  • Protein-protein
  • Target-drug
  • Enzyme-inhibitor
  • Antibody-antigen
  • Protein-DNA
  • Protein-lipid
  • Small molecule-small molecule
Applications include:
  • Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules;
  • Lead optimization;
  • Enzyme kinetics;
  • Assessment of the effect of molecular structure changes on binding mechanisms;
  • Assessment of biological activity.
info :
Brand : GE Healthcare Model : VP-ITCell Inventory Number : FIM2145 Room : P2B45