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Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, Taiwan. After immunostaining, stalls were mounted in Fluoro-Guard Antifade Reagent (Bio-Rad, Hemel Hempstead, UK) and examined with a confocal laser scanning microscope (Radiance 2100, Bio-Rad or C1si, Nikon, Kawasaki, Japan).
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Materials and Methods Immunization and phage unmask library construction Two llamas were immunized with either a manufactured Vpr peptide (MEQAPEDQGPQREPYNDWTLELLEELKNEAVRHFPRIWLHSLGQHIYETYGDTWTGVEALIRILQQLLFIHFRIGCRHSRIGIIQQRRTRNGASKS) or recombinant CA tadalafil and breast feeding produced in E. coli and purified via its GST Tag which was eliminated in thrombin cleavage.
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