STEP 1: Select Option
Basic Search
(to get information about exons, introns,isoforms, splicing events, translation)
In silico PCR
(to get amplicon length, and primer graphical location)
Check for primer cross-amplification
(search across all alternative splicing patterns)
Search isoform-specific sequences for primer design
(to get isoform-specific exon-juntions)
Search transcript sequence
Decompose transcript sequence in ExonMine exons
STEP 2: Input Values
Forward:
Reverse:
Enter Transcript Sequence:
Enter Isoform Number (from Basic Search, ex: 2):
