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zeiss_lsm_710

Location: Room P3-A-10 ( 47331)
Manufacturer: Carl Zeiss MicroImaging
Model: LSM 710
Nickname: "710"
Software: ZEN 2012
Year: 2010
SN: 2503000400
 
 
 
Zeiss LSM 710 Booking
Zeiss LSM 710 Quality Control
Zeiss LSM 710 Usage Statistics

Microscope overview

The Zeiss LSM 710 is a confocal point-scanning microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control (but no stage incubator for CO2 supply). Its scanning unit has a spectral recycling loop, two sensitive PMT detectors and a filter-free spectral separation module that can be continuously set over the entire wavelength range (i.e. you can specify which wavelength range you want to detect). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the Zeiss Cell Observer or the Zeiss Axiovert 200M. Image resolution and detection sensitivity are higher than faster confocal systems such as the spinning disks 3i Marianas SDC and Zeiss Cell Observer SD, but image acquisition is slower. If you need to use a 594 nm laser or increased resolution and sensitivity, or are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the Zeiss LSM 880 confocal point-scanning system with Airyscan and Definite Focus. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus.

Click on the image on the right to see the system beam path in higher detail

Data files older than 3 months will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

Booking Rules

The Zeiss LSM 710 booking is divided in three daily slots:

9:00 to 13:00 13:00 to 17:00 17:00 to 21:00
  1. A user can book at maximum 2 sessions per week
  2. Each session must fit inside one of the three daily slots (i.e. a session booked from 11:00 to 14:00 counts as 2 sessions)
  3. The above rules do not apply for weekends and for work days before 9:00 and after 21:00
  4. The above rules do not apply for sessions booked on the very same day or 24 hours ahead
  5. Exceptions to these rules require approval from José Rino.
  6. Users must indicate which lasers they are going to use in the booking Description field
  7. Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR)

System components

LASERs

Laser Unit Wavelength Maximum Power Current Status
Diode 405-30 405 nm 30 mW ok
Argon 458, 488 and 514 nm 25 mW ok
DPSS 561-10 561 nm 15 mW ok
HeNe633 633 nm 5 mW ok

Objectives

Magnification Model Immersion NA WD (mm) Reference
10x EC Plan-Neofluar Air 0.30 5.2 420340-9901-000
20x Plan-Apochromat Air 0.80 0.55 420650-9901-000
40x EC Plan-Neofluar DIC Oil 1.30 0.20 440452-9903-000
63x Plan-Apochromat DIC Oil 1.40 0.19 440762-9904-000

Upon request:

Magnification Model Immersion NA WD (mm) Reference
100x Plan-Apochromat DIC Oil 1.40 0.17 440782-9902-000

Filtersets (Ocular)

Position Filterset Reference Excitation Emission
1 Green FS38 450-490 nm 500-550 nm
2 Red FS43 533-558 nm 570-640 nm
3 Blue FS49 G 365 nm 420-470 nm

Microscope Turn On Procedure

  • Turn on the MAIN SWITCH
  • Turn on the two switches: SYSTEMS/PC and COMPONENTS

switches_710.jpg

  • Turn on the metal halide fluorescent light source (if needed)

halide_710.jpg

  • Turn on the Ar laser using the key (if needed)

laser_key_710.jpg

  • Turn on the computer
  • Log in to Windows (Bioimaging User)
  • Login with Bioimaging User account
  • Check that the computer is connected to an Unidentified network (click on the network icon in the taskbar)

  • Switch the Ar laser from idle ton run (don't change the current)

laser_run_710.jpg

  • Start the ZEN software

Microscope Turn Off Procedure

If there is another user for this microscope in the next hour:

  • Close ZEN, leave the lasers on, log off the computer
  • Clean up immersion objectives

Else:

  • Turn off the 561 nm laser and incubator in the software (if used)
  • Switch the Ar laser from run to idle (if used)
  • Turn off the Ar laser using the key (if used)
  • Clean up immersion objectives
  • Close ZEN, shutdown the computer.
  • Turn off the metal halide fluorescence light source
  • Turn off the two switches: SYSTEMS/PC and COMPONENTS
  • Wait 5 min. for Ar laser cooldown
  • Turn off the MAIN SWITCH

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zeiss_lsm_710.txt · Last modified: 2020/01/15 12:22 by bioimaging