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zeiss_axiovert_200m

Location: Room P2-A-24 ( 47219)
Manufacturer: ZEISS Microscopy
Model: Axiovert 200M
Nickname: "Axiovert"
Software: Metamorph 7.8.0.0
Year: 2003
SN: ZCA522000494
 
Data will be deleted after: 1 month  
 
Zeiss Axiovert 200M Quality Control
Zeiss Axiovert 200M Usage Statistics

Microscope overview

The Zeiss Axiovert 200M is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging applications. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Photometrics CoolSNAP HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. It is also equipped with a color camera (Leica DFC450) for acquisition of histological stains with tile and stitching imaging.

Data files older than 1 month will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

System components

Filtersets

Position Filterset Reference Excitation Dichroic Emission
1 Blue FS01 359-371 nm 395 nm > 397 nm
2 Green BP FS10 450-490 nm 510 nm 515-565 nm
3 Red FS20 540-552 nm 560 nm 575-640 nm
4 Far Red Cy5-4040C-000 608-648 nm 660 nm 672-712 nm
5 A none

Upon request:

Position Filterset Reference Excitation Dichroic Emission
2 Green LP FS09 450-490 nm 510 nm > 515 nm

Objectives

Magnification Model Immersion NA WD (mm) pixel size* (μm) 10 μm = (color) 10 μm = Reference
10x EC Plan-Neofluar Air 0.30 5.20 1.00 10 pixels 23.3 pixels 420340-9900-000
20x EC Plan-Neofluar Air 0.50 2.00 0.48 21 pixels 46.7 pixels 440340-9904-000
40x EC Plan-NeoFluar Air 0.75 0.71 0.24 41 pixels 93.7 pixels 440350-9903-000
63x Plan-Apochromat DIC Oil 1.40 0.19 0.15 65 pixels - 420782-9900-000
100x α Plan-Apochromat DIC Oil 1.46 0.11 0.09 105 pixels - 420792-9800-000

Upon request:

Magnification Model Immersion NA WD (mm) pixel size* (μm) 10 μm = Reference
1.25x EC Epiplan-Neofluar Air 0.035 4 7.58 1.3 pixels 422310-9900-000
5x EC Plan-NeoFluar Air 0.16 18.5 2.00 5 pixels 420330-9901-000
40x Plan-Neofluar Ph3 Oil 1.30 0.20 0.24 41 pixels 440451-9903-000
63x Plan-Apochromat Ph3 Oil 1.4 0.19 0.15 65 pixels 420781-9910-000

* pixel sizes for binning = 1

Cameras

Model Type Frame Size Pixel Size (µm)
Photometrics Coolsnap HQ CCD 1392 x 1040 6.45 x 6.45
Leica DFC450 color CCD 2560 × 1920 3.4 x 3.4

Stage

For automated acquisition of 96 well plates use: X = -9014 μm Y = -8995 μm

System Turn On Procedures

  • Turn on the fluorescent light power source (if used)

  • Turn on the Prior stage controller

  • Turn on the microscope

  • Turn on the CCD camera

  • Turn on the computer
  • Log in to Windows (Bioimaging User)
  • Start the Metamorph software
  • If the software doesn't detect the camera, power cycle the camera and try again

Microscope Turn Off procedures

If there is another user for this microscope in the next hour:

  • Leave the fluorescent lamp on (if used)
  • Clean up immersion objectives

Else:

  • Clean up immersion objectives
  • Shutdown the computer
  • Turn off the camera
  • Turn off the Prior stage controller
  • Turn off the microscope
  • Turn off the fluorescent lamp (if used)

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zeiss_axiovert_200m.txt · Last modified: 2024/07/25 01:29 by bioimaging