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Location: Room P3-A-10 ( 47331) |
The Zeiss Cell Observer is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our deconvolution server Brainstorm to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners Zeiss LSM 710 and Zeiss LSM 880 or spinning disks 3i Marianas SDC and Zeiss Cell Observer SD. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus. If you have FISH or other fixed fluorescence slides try the Leica DM5000B instead.
Data files older than 1 month will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
The Zeiss Cell Observer booking is divided in three daily slots:
9:00 to 13:00 | 13:00 to 17:00 | 17:00 to 21:00 |
Magnification | Model | Type | NA | WD (mm) |
---|---|---|---|---|
10x | EC Plan-Neofluar | Dry | 0.30 | 5.2 |
20x | Plan-Apochromat | Dry | 0.80 | 0.55 |
40x | EC Plan-NeoFluar | Dry | 0.75 | 0.71 |
63x | Plan-Apochromat | Oil | 1.40 | 0.19 |
Upon request:
Magnification | Model | Type | NA | WD (mm) |
---|---|---|---|---|
1.25x | EC Epiplan-Neofluar | Dry | 0.035 | 4 |
5x | EC Plan-NeoFluar | Dry | 0.16 | 18.5 |
20x | EC Plan-Neofluar Ph 2 | Dry | 0.50 | 2 |
40x | Plan-Apochromat | Dry | 0.95 | 0.25 |
40x | Plan-Neofluar Ph3 | Oil | 1.30 | 0.20 |
63x | Plan-Apochromat Ph3 | Oil | 1.4 | 0.19 |
100x | Plan-Apochromat | Oil | 1.40 | 0.17 |