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Location: Room P3-A-44
Manufacturer: Leica Microsystems
Model: DM5000 B
Nickname: "5000B"
Software: Leica DC Twain
Year: 2006
SN: 269835
Leica DM5000B Booking
Leica DM5000B Usage Statistics
Leica DM5000B Repair History

Microscope overview

The Leica DM5000B is an automated upright widefield fluorescence microscope ideal for imaging FISH or other fixed fluorescence slides. The microscope is prepared for brightfield, phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). It has a monochrome CCD camera for fluorescence image acquisition. If you want to image live samples and/or perform time-lapse experiments check out the Zeiss Axiovert 200M instead. If you need a color camera for brightfield images, try the Leica DM2500.

System components


Filterset Reference Excitation Emission Fluorochromes
Blue A4 320-400 nm 430-510 nm DAPI, Hoechst, Alexa350
Green GFP 450-490 nm 500-550 nm GFP, Cy2, FITC
Red N21 515-560 nm > 590 nm Rhodamine, Cy3, Texas Red


Magnification Model Type NA WD (mm) pixel size* (μm) 10 μm = BF PH DIC POL
5x HC PL FLUOTAR Dry 0.15 12 2.00 5 pixels Yes No No Yes
10x HC PL FLUOTAR Dry 0.30 11 1.00 10 pixels Yes Yes No Yes
20x HC PL FLUOTAR Dry 0.50 1.15 0.53 19 pixels Yes Yes Yes Yes
20x HC PLAN APO Dry 0.70 0.59 0.53 19 pixels Yes No Yes Yes
40x HCX PL FLUOTAR Dry 0.75 0.40 0.26 39 pixels Yes Yes Yes Yes
63x HCX PL APO Oil 1.4-0.60 0.1 0.16 63 pixels Yes No Yes Yes
100x HCX PL FLUOTAR Oil 1.3 0.13 0.10 96 pixels Yes No Yes Yes

* pixel size for 1300 x 1030 images

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leica_dm5000b.txt · Last modified: 2017/07/13 16:27 by bioimaging