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Location: Lab 16 (floor 0)
Manufacturer: Leica Microsystems
Model: DM5000 B
Nickname: "5000B"
Year: 2006
SN: 269835
Leica DM5000B Quality Control
Leica DM5000B Usage Statistics

Microscope overview

The Leica DM5000B is an automated upright widefield fluorescence microscope ideal for checking FISH or other fixed fluorescence slides. The microscope is prepared for brightfield, phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). If you want to image live samples and/or perform time-lapse experiments check out the Zeiss Axiovert 200M or the Zeiss Cell Observer instead. If you need a color camera for brightfield images, try the Leica DM2500.

System components


Filterset Reference Excitation Dichroic Emission Fluorochromes
Blue A4 340-380 nm 400 nm 450-490 nm DAPI, Hoechst, Alexa350
Green GFP 450-490 nm 500 nm 500-550 nm GFP, FITC, Alexa488
Red N2.1 515-560 nm 580 nm > 590 nm Rhodamine, Cy3, Texas Red


Magnification Model Immersion NA WD (mm) BF PH DIC POL Reference
5x HC PL FLUOTAR Air 0.15 12.00 506504
10x HC PL FLUOTAR Air 0.30 11.00 506507
20x HC PL FLUOTAR Air 0.50 1.15 506506
20x HC PLAN APO Air 0.70 0.59 506166
40x HCX PL FLUOTAR Air 0.75 0.40 506145
63x HCX PL APO Oil 1.4-0.60 0.10 506187

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leica_dm5000b.txt · Last modified: 2023/08/06 13:30 by bioimaging