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Location: Room P2-A-22 ( 47217) |
The Zeiss Axiovert 200M is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Photometrics Coolsnap HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use PPBI Huygens RM deconvolution server to significantly improve image resolution even in thick samples. If you are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the Zeiss Cell Observer with Definite Focus. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners Zeiss LSM 710 and Zeiss LSM 880 or the spinning disks 3i Marianas SDC and Zeiss Cell Observer SD. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus. If you have FISH or other fixed fluorescence slides try the Leica DM5000B instead.
Data files older than 1 month will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
The Axiovert 200M booking is divided in three daily slots:
9:00 to 13:00 | 13:00 to 17:00 | 17:00 to 21:00 |
Position | Filterset | Reference | Excitation | Emission |
---|---|---|---|---|
1 | Blue | FS01 | 359-371 nm | > 397 nm |
2 | Green BP | FS10 | 450-490 nm | 515-565 nm |
3 | Red | FS20 | 540-552 nm | 575-640 nm |
4 | Far Red | Cy5-4040C-000 | 608-648 nm | 672-712 nm |
5 | A | none | none | none |
Upon request:
Position | Filterset | Reference | Excitation | Emission |
---|---|---|---|---|
2 | Green LP | FS09 | 450-490 nm | > 515 nm |
Magnification | Model | Immersion | NA | WD (mm) | pixel size* (μm) | 10 μm = | (color) 10 μm = | Reference |
---|---|---|---|---|---|---|---|---|
10x | EC Plan-Neofluar | Air | 0.30 | 5.2 | 1.00 | 10 pixels | 23.3 pixels | 420340-9901-000 |
20x | EC Plan-Neofluar | Air | 0.50 | 2 | 0.48 | 21 pixels | 46.7 pixels | 420650-9901-000 |
40x | EC Plan-NeoFluar | Air | 0.75 | 0.71 | 0.24 | 41 pixels | 93.7 pixels | |
63x | Plan-Apochromat | Oil | 1.40 | 0.19 | 0.15 | 65 pixels | - |
Upon request:
Magnification | Model | Type | NA | WD (mm) | pixel size* (μm) | 10 μm = |
---|---|---|---|---|---|---|
1.25x | EC Epiplan-Neofluar | Dry | 0.035 | 4 | 7.58 | 1.3 pixels |
5x | EC Plan-NeoFluar | Dry | 0.16 | 18.5 | 2.00 | 5 pixels |
20x | EC Plan-Neofluar Ph 2 | Dry | 0.50 | 2 | 0.48 | 21 pixels |
40x | Plan-Neofluar Ph3 | Oil | 1.30 | 0.20 | 0.24 | 41 pixels |
63x | Plan-Apochromat Ph3 | Oil | 1.4 | 0.19 | 0.15 | 65 pixels |
* pixel sizes for binning = 1
For automated acquisition of 96 well plates use: X = -9014 μm Y = -8995 μm