User Tools

Site Tools


zeiss_lumar_v12

This is an old revision of the document!


Location: Room P0-B-32
Manufacturer: Carl Zeiss MicroImaging
Model: SteREO Lumar V.12Axio Zoom.V16
Nickname: "Lumar"
Software: AxioVision
Year: 2009
SN: 3918000768
 
 
 
 
Zeiss Lumar V12 Booking
Zeiss Lumar V12 Usage Statistics

Microscope overview

The Zeiss Lumar V12 is a fluorescence stereo microscope suitable for high resolution 3D analysis of large specimens in fluorescent and transmitted light. The system is equipped with fluorescence filters for GFP, Cy3 and Cy5.5 and a sensitive cooled CCD camera (Hamamatsu ORCA-R2) that allows you to capture weak fluorescent signals and minimize photobleaching in light sensitive samples. With the Zeiss Lumar V12 you can perform multi-channel, Z-stack and time-lapse image acquisition of your samples, using two different objectives with different working distances and magnification values. If you need higher magnifications, check out the Leica DM5000B, the Zeiss Axiovert 200M or the Zeiss Cell Observer widefield microscopes.

Data files older than 3 months will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

System components

Filtersets

Position Filterset Reference Excitation Dichroic Emission
1 Empty
2 GFP FS13 460-480 nm 495 nm 505-530 nm
3 Cy3 FS43 532-557 nm 570 nm 570-640 nm
4 Cy5.5 FS32 652-677nm 695 nm 700-750 nm

Objectives

Magnification Model WD (mm) About
0.8x NeoLumar S 80 for visual magnification from 6.4x to 80x with eyepieces 10x

Upon Request:

Magnification Model WD (mm) About
1.5x NeoLumar S 30 for visual magnification from 12x to 150x with eyepieces 10x

Camera

Model Type Frame Size Pixel Size (µm)
Hamamatsu ORCA-R2 CCD 1344 X 1024 6.45 x 6.45

Software Modules

  • Multichannel Acquisition
  • Z-stack Acquisition
  • Extended Focus

Back to the Resources page

zeiss_lumar_v12.1591032416.txt.gz · Last modified: 2020/06/01 19:26 by bioimaging