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Location: Room P2-A-22 ( |
The Zeiss LSM 880 with Airyscan is a confocal point-scanning microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply. Its Airyscan 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the Zeiss Cell Observer WF or the Zeiss Axiovert 200M. Image resolution with Airyscan is higher than the Zeiss LSM 710 and detection sensitivity is also higher than faster confocal systems such as the spinning disk 3i Marianas SDC or the line scanning Zeiss LSM 7 Live. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus.
Laser Unit | Wavelength | Maximum Power | Current Status |
---|---|---|---|
Sapphire 488-100 | 488 nm | 100 mW | ok |
DPSS 561-40 | 561 nm | 40 mW | ok |
Magnification | Model | Type | NA | WD (mm) |
---|---|---|---|---|
10x | W Achroplan Ph1 | Water dipping | 0.30 | 3.1 |
10x | Plan-Neofluar | Dry | 0.30 | 5.5 |
20x | W Plan-Apochromat | Water | 1.00 | 1.8 |
40x | W Achroplan | Water dipping | 0.80 | 3.6 |
40x | Plan-Neofluar Ph3 | Oil | 1.30 | 0.20 |
Position | Filterset | Reference | Excitation | Emission |
---|---|---|---|---|
2 | Green | FS10 | 450-490 nm | 515-565 nm |
3 | Red | FS15 | 540-552 nm | > 590 nm |
4 | Blue (for ablation*) | FS49 | none | 420-470 nm |
* Excitation filter has been removed
SYSTEM/PC
and COMPONENTS
connected
stateAfter the 30 min:
connected
stateAfter the 2 hours:
If there's another user for this microscope in the next two hours:
Else: