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Table of Contents
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Location: Room P0-B-32 |
Leica DM5000B Booking
Leica DM5000B Usage Statistics
Microscope overview
The Leica DM5000B is an automated upright widefield fluorescence microscope ideal for imaging FISH or other fixed fluorescence slides. The microscope is prepared for brightfield, phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). It has a monochrome CCD camera for fluorescence image acquisition. If you want to image live samples and/or perform time-lapse experiments check out the Zeiss Axiovert 200M or the Zeiss Cell Observer instead. If you need a color camera for brightfield images, try the Leica DM2500.
Data files older than 3 months will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
System components
Filtersets
| Filterset | Reference | Excitation | Emission | Fluorochromes |
|---|---|---|---|---|
| Blue | A4 | 340-380 nm | 450-490 nm | DAPI, Hoechst, Alexa350 |
| Green | GFP | 450-490 nm | 500-550 nm | GFP, FITC, Alexa488 |
| Red | N2.1 | 515-560 nm | > 590 nm | Rhodamine, Cy3, Texas Red |
Objectives
| Magnification | Model | Immersion | NA | WD (mm) | pixel size* (μm) | 10 μm = | BF | PH | DIC | POL | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 5x | HC PL FLUOTAR | Air | 0.15 | 12 | 2.00 | 5 pixels | Yes | No | No | Yes | |
| 10x | HC PL FLUOTAR | Air | 0.30 | 11 | 1.00 | 10 pixels | Yes | Yes | No | Yes | |
| 20x | HC PL FLUOTAR | Air | 0.50 | 1.15 | 0.53 | 19 pixels | Yes | Yes | Yes | Yes | |
| 20x | HC PLAN APO | Air | 0.70 | 0.59 | 0.53 | 19 pixels | Yes | No | Yes | Yes | |
| 40x | HCX PL FLUOTAR | Air | 0.75 | 0.40 | 0.26 | 39 pixels | Yes | Yes | Yes | Yes | |
| 63x | HCX PL APO | Oil | 1.4-0.60 | 0.1 | 0.16 | 63 pixels | Yes | No | Yes | Yes |
* pixel size for 1300 x 1030 images
Upon request:
| Magnification | Model | Type | NA | WD (mm) | pixel size* (μm) | 10 μm = | BF | PH | DIC | POL |
|---|---|---|---|---|---|---|---|---|---|---|
| 100x | HCX PL FLUOTAR | Oil | 1.3 | 0.13 | 0.10 | 96 pixels | Yes | No | Yes | Yes |
