Table of Contents
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Leica DMR Booking
Leica DMR Usage Statistics
Leica DMR Repair HistoryMicroscope overview
The Leica DMR is an upright widefield fluorescence microscope equipped with phase contrast objectives and a color camera for brightfield/fluorescence imaging. If its color camera is not sensitive enough for your fluorescent sample try the Leica DM5000B instead. If your sample is not fluorescent, the Leica DM2500 is also an option for brightfield imaging. If you want to image live samples and/or perform time-lapse experiments check out the Zeiss Axiovert 200M instead.
System components
Filtersets
| Filterset | Reference | Excitation | Emission | Fluorochromes |
|---|---|---|---|---|
| Blue | A4 | 320-400 nm | 430-510 nm | DAPI, Hoechst, Alexa350 |
| Green | L5 | 440-520 nm | 497-557 nm | GFP, Cy2, FITC |
| Red BP | Y3 | 515-575 nm | 535-685 nm | Rhodamine, Cy3, Texas Red |
| Red LP | N2.1 | 515-560 nm | > 590 nm | Rhodamine, Cy3, Cy5 |
Objectives
| Magnification | Model | Type | NA | WD (mm) |
|---|---|---|---|---|
| 5x | N PLAN | Dry | 0.12 | 14 |
| 10x | HC PL FLUOTAR | Dry | 0.30 | 11 |
| 20x | HC PL FLUOTAR | Dry | 0.50 | 1.15 |
| 40x | HCX PL FLUOTAR | Dry | 0.75 | 0.40 |
| 63x | HCX PL APO | Oil | 1.32-0.60 | 0.1 |