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Location: Room P2-B-42 ( 47246)
The Zeiss LSM 7 Live confocal line-scanning microscope is a fast imaging system able to analyze high-speed processes with a time resolution of just a few microseconds. It is an upright microscope equipped with water dipping objectives specially suited for live samples in aqueous media. The scanning unit uses a laser beam with a rectangular cross-section to illuminate a whole line in the sample, instead of a single point. This makes image acquisition by its array CCD detectors faster than conventional PMT light detection but it also lowers the Z-resolution due to the usage of slits instead of conventional pinholes. Note that the system is equipped with two lasers (488 and 561 nm) but dual color imaging is not advised since there are no bandpass filters in the system (channel crosstalk is likely to occur). A UV laser controlled by galvanometric mirrors is available for precise ablation in user-defined regions of interest. The spatial resolution of this system is not as high as a point-scanning confocal system such as the Zeiss LSM 880 or Zeiss LSM 710 but it is much faster than these two systems, achieving scanning speeds which may be even higher than the 3i Marianas SDC spinning disk if the acquisition is performed only in a few lines. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus.
|Laser Unit||Wavelength||Maximum Power||Current Status|
|Sapphire 488-100||488 nm||100 mW||ok|
|DPSS 561-40||561 nm||40 mW||ok|
|2||Green||FS10||450-490 nm||515-565 nm|
|3||Red||FS15||540-552 nm||> 590 nm|
|4||Blue (for ablation*)||FS49||none||420-470 nm|
* Excitation filter has been removed
After the 30 min:
After the 2 hours:
If there's another user for this microscope in the next two hours: