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Location: Room P3-A-10 ( 47311) |
The Zeiss LSM 710 is a confocal point-scanning microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control (but no stage incubator for CO2 supply). Its scanning unit has a spectral recycling loop, two sensitive PMT detectors and a filter-free spectral separation module that can be continuously set over the entire wavelength range (i.e. you can specify which wavelength range you want to detect). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the Zeiss Cell Observer WF or the Zeiss Axiovert 200M. Image resolution and detection sensitivity are higher than faster confocal systems such as the spinning disk 3i Marianas SDC or the Zeiss LSM 7 Live, but image acquisition is slower. If you need to use a 594 nm laser or increased resolution and sensitivity, check the Zeiss LSM 880 confocal point-scanning system. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus.
The Zeiss LSM 710 booking is divided in three daily slots:
9:00 to 13:00 | 13:00 to 17:00 | 17:00 to 21:00 |
Laser Unit | Wavelength | Maximum Power | Current Status |
---|---|---|---|
Diode 405-30 | 405 nm | 30 mW | ok |
Argon | 458, 488 and 514 nm | 25 mW | ok |
DPSS 561-10 | 561 nm | 15 mW | ok |
HeNe633 | 633 nm | 5 mW | ok |
Magnification | Model | Type | NA | WD (mm) |
---|---|---|---|---|
10x | EC Plan-Neofluar | Dry | 0.30 | 5.2 |
20x | Plan-Apochromat | Dry | 0.80 | 0.55 |
40x | EC Plan-Neofluar | Oil | 1.30 | 0.20 |
63x | Plan-Apochromat | Oil | 1.40 | 0.19 |
Upon request:
Magnification | Model | Type | NA | WD (mm) |
---|---|---|---|---|
25x | LCI Plan-NeoFluar | Oil/Glyc/W | 0.8 | 0.21 |
100x | Plan-Apochromat | Oil | 1.40 | 0.17 |
MAIN SWITCH
SYSTEMS/PC
and COMPONENTS
connected
stateidle
ton run
(don't change the current
)If there's another user for this microscope in the next hour:
Else:
run
to idle
(if used)SYSTEMS/PC
and COMPONENTS
MAIN SWITCH
.