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Table of Contents
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Location: Room P2-A-24 ( |
47219) 
Nikon Eclipse Ti Quality Control
Nikon Eclipse Ti Usage Statistics
Microscope overview
The Nikon Eclipse Ti is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using NIS-Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Andor Neo 5.5 monochrome cooled sCMOS camera ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use PPBI Huygens RM deconvolution server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners Zeiss LSM 710 and Zeiss LSM 880 or spinning disks 3i Marianas SDC and Zeiss Cell Observer SD. If your personal computer cannot handle all the data you collected, check out the Colossus.
- Microscope: Nikon Eclipse Ti
- Camera: Andor Neo 5.5 sCMOS
Data files older than 3 months will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
System components
Filtersets Turret 1 (top)
| Position | Filterset | Reference | Excitation | Dichroic | Emission |
|---|---|---|---|---|---|
| 1 | Empty | ||||
| 2 | DAPI | MBE41300 | 340-380 nm | 400 nm | 435-485 nm |
| 3 | FITC | MBE44720 | 465-495 nm | 505 nm | 515-555 nm |
| 4 | TRITC | MBE45600 | 528-553 nm | 565 nm | 590-650 nm |
| 5 | Cy5 | 2SK4CY50 | 608-648 nm | 660 nm | 672-712 nm |
| 6 | Empty | ||||
Filtersets Turret 2 (bottom)
| Position | Filterset |
|---|---|
| 1 | Empty |
| 2 | Empty |
| 3 | Empty |
| 4 | Empty |
| 5 | Empty |
| 6 | Fura-2 |
Objectives
| Magnification | Model | Immersion | NA | WD (mm) | Reference |
|---|---|---|---|---|---|
| 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | MRH10101 |
| 20x | CFI S Plan Fluor ELWD ADM Ph1 | Air | 0.45 | 6.90 - 8.20 | MRH48230 |
| 40x | CFI CFI Plan Apochromat Lambda D | Air | 0.95 | 0.21 | MRH70470 |
| 40x | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | MRH48430 |
| 60x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | |
| 100x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 |
Upon request:
| Magnification | Model | Immersion | NA | WD (mm) | Reference |
|---|---|---|---|---|---|
| 100x | CFI Plan | Water | 1.10 | 2.50 | MRL07920 |
Camera
| Model | Type | Frame Size | Pixel Size (µm) |
|---|---|---|---|
| Andor Neo 5.5 sCMOS | CCD | 2560 x 2160 | 6.5 x 6.5 |
