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nikon_eclipse_te2000e

Location: Room P3-A-5
Manufacturer: Nikon Instruments
Model: Eclipse TE2000-E
Nickname: "Asterix"
Software: Metamorph / NIS-Elements
Year: 2007
SN: 596701
 
 
 
 
Nikon Eclipse TE2000-E Booking
Nikon Eclipse TE2000-E Usage Statistics

Microscope overview

The Nikon Eclipse TE2000-E is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a CoolSNAP EZ cooled monochrome CCD camera for fast, high-resolution imaging. You can perform Z-stack acquisition and use PPBI Huygens RM deconvolution server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners Zeiss LSM 710 and Zeiss LSM 880 or spinning disks 3i Marianas SDC and Zeiss Cell Observer SD. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus.

Data files older than 3 months will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

System components

Filtersets

Position Filterset Reference Excitation Dichroic Emission
1 DAPI MBE41300 340-380 nm 400 nm 435-485 nm
2 TRITC MBE45600 528-553 nm 565 nm 577-632 nm
3 AF none
4 Empty
5 GFP MBE44720 465-495 nm 505 nm 515-555 nm
6 Cy5 2SK4CY50 608-648 nm 660 nm 672-712 nm

Objectives

Magnification Model Immersion NA WD (mm) Reference
10x CFI Plan Fluor DLL Ph1 Air 0.30 16.00 MRH10101
20x CFI S Plan Fluor ELWD ADM Ph1 Air 0.45 6.90 - 8.20 MRH48230
40x CFI Plan Fluor DLL Ph2 Air 0.75 0.66 MRH10401
60x CFI S Plan Apo DM Ph3 Oil 1.40 0.13 MRD31605

Upon request:

Magnification Model Immersion NA WD (mm) Reference
40x CFI S Plan Fluor ELWD ADL Ph 2 Air 0.60 2.70 - 3.70
100x CFI Plan Water 1.10 2.50 MRL07920

Camera

Model Type Frame Size Pixel Size (µm)
CoolSNAP EZ mono CCD CCD 1392 x 1040 6.45 x 6.45

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nikon_eclipse_te2000e.txt · Last modified: 2020/06/01 19:25 by bioimaging