zeiss_lsm_880
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zeiss_lsm_880 [2025/12/08 22:55] bioimaging [How to start your session] |
zeiss_lsm_880 [2025/12/12 14:56] (current) bioimaging [Filtersets (for eyepieces)] |
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| ==== LASERs ==== | ==== LASERs ==== | ||
| + | |||
| ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
| - | | violet | <fc #a600ff>405 nm</fc> | 30 mW | | + | | Violet | <fc #a600ff>405 nm</fc> | 30 mW | |
| | Argon | <fc #0071ff>458</fc>, <fc #00f7ff>488</fc> and <fc #1aff00>514</fc> nm | 25 mW | | | Argon | <fc #0071ff>458</fc>, <fc #00f7ff>488</fc> and <fc #1aff00>514</fc> nm | 25 mW | | ||
| - | | yellow-green | <fc #c6ff00>561 nm</fc> | 20 mW | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 20 mW | |
| - | | orange | <fc #ffd200>594 nm</fc> | 2 mW | | + | | Orange | <fc #ffd200>594 nm</fc> | 2 mW | |
| - | | red | <fc #ff4200>639 nm</fc> | 5 mW | | + | | Red | <fc #ff4200>639 nm</fc> | 5 mW | |
| + | |||
| + | ==== Filtersets (for eyepieces) ==== | ||
| + | |||
| + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ||
| + | | 2 | Cyan | 426-446 nm | 455 nm | <fc #00d5ff>460-500 nm</fc> | [[https://www.chroma.com/products/sets/49001-et-ecfp|49001]] | | ||
| + | | 3 | Green | 450-490 nm | 493 nm | <fc #4aff00>500-550 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | | ||
| + | | 4 | Orange-Red | 533-558 nm | 570 nm | <fc #ffad00>570-640 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | | ||
| + | | 5 | Blue | G 365 nm | 395 nm | <fc #0028ff>420-470 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| + | |||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| | <fc #fef042>10x</fc> | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | | <fc #fef042>10x</fc> | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | ||
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| | <fc #0aa05c>25x</fc> | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | | <fc #0aa05c>25x</fc> | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | ||
| | <fc #01b3f5>40x</fc> | Plan-Apochromat Corr | Dry | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | | <fc #01b3f5>40x</fc> | Plan-Apochromat Corr | Dry | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | ||
| - | ==== Filtersets (for eyepieces) ==== | ||
| - | |||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
| - | | 2 | Cyan | [[https://www.chroma.com/products/sets/49001-et-ecfp|49001]] | <fc #1d00ff>426-446 nm</fc> | <fc #0061ff>455 nm</fc> | <fc #00d5ff>460-500 nm</fc> | | ||
| - | | 3 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | <fc #00a9ff>450-490 nm</fc> | <fc #00ffcb>493 nm</fc> | <fc #4aff00>500-550 nm</fc> | | ||
| - | | 4 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | <fc #9cff00>533-558 nm</fc> | <fc #e1ff00>570 nm</fc> | <fc #ffad00>570-640 nm</fc> | | ||
| - | | 5 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | <fc #cc00cc>G 365 nm</fc> | <fc #b800e5>395 nm</fc> | <fc #0028ff>420-470 nm</fc> | | ||
| ===== How to start your session ===== | ===== How to start your session ===== | ||
zeiss_lsm_880.1765230930.txt.gz · Last modified: 2025/12/08 22:55 by bioimaging
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