zeiss_lsm_880
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zeiss_lsm_880 [2025/07/28 16:34] bioimaging [Booking Rules] |
zeiss_lsm_880 [2025/12/12 14:56] (current) bioimaging [Filtersets (for eyepieces)] |
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| {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | ||
| - | ===== Booking Rules ===== | + | |
| - | - **Users** can book at maximum **8 hours per week** | + | |
| - | - This usage restriction does not apply for **weekends** and for working days **before 9:00** and **after 18:00** | + | |
| - | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | + | |
| ===== System components ===== | ===== System components ===== | ||
| ==== LASERs ==== | ==== LASERs ==== | ||
| + | |||
| ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
| - | | Diode 405-30 | 405 nm | 30 mW | | + | | Violet | <fc #a600ff>405 nm</fc> | 30 mW | |
| - | | Argon | 458, 488 and 514 nm | 25 mW | | + | | Argon | <fc #0071ff>458</fc>, <fc #00f7ff>488</fc> and <fc #1aff00>514</fc> nm | 25 mW | |
| - | | DPSS 561-20 | 561 nm | 20 mW | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 20 mW | |
| - | | HeNe594 | 594 nm | 2 mW | | + | | Orange | <fc #ffd200>594 nm</fc> | 2 mW | |
| - | | HeNe633 | 633 nm | 5 mW | | + | | Red | <fc #ff4200>639 nm</fc> | 5 mW | |
| + | |||
| + | ==== Filtersets (for eyepieces) ==== | ||
| + | |||
| + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ||
| + | | 2 | Cyan | 426-446 nm | 455 nm | <fc #00d5ff>460-500 nm</fc> | [[https://www.chroma.com/products/sets/49001-et-ecfp|49001]] | | ||
| + | | 3 | Green | 450-490 nm | 493 nm | <fc #4aff00>500-550 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | | ||
| + | | 4 | Orange-Red | 533-558 nm | 570 nm | <fc #ffad00>570-640 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | | ||
| + | | 5 | Blue | G 365 nm | 395 nm | <fc #0028ff>420-470 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| + | |||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | + | | <fc #fef042>10x</fc> | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | |
| - | | 20x | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] | | + | | <fc #0aa05c>20x</fc> | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] | |
| - | | 40x | C-Apochromat Corr | Water | 1.20 | 0.28 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421767-9970-000/Objective-C-Apochromat-40x-1.20-W-Corr-M27|421767-9970-000]] | | + | | <fc #01b3f5>40x</fc> | C-Apochromat Corr | Water | 1.20 | 0.28 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421767-9970-000/Objective-C-Apochromat-40x-1.20-W-Corr-M27|421767-9970-000]] | |
| - | | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | + | | <fc #01b3f5>40x</fc> | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | |
| - | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | + | | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | |
| Line 66: | Line 74: | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | + | | <fc #0aa05c>25x</fc> | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | |
| - | | 40x | Plan-Apochromat Corr | Dry | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | + | | <fc #01b3f5>40x</fc> | Plan-Apochromat Corr | Dry | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | |
| - | ==== Filtersets (Ocular) ==== | + | |
| - | + | ||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | |
| - | | 2 | Cyan | [[https://www.chroma.com/products/sets/49001-et-ecfp|49001]] | 426-446 nm | 455 nm | 460-500 nm | | + | |
| - | | 3 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 495 nm | 500-550 nm | | + | |
| - | | 4 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 533-558 nm | 570 nm | 570-640 nm | | + | |
| - | | 5 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | G 365 nm | 395 nm | 420-470 nm | | + | |
| ===== How to start your session ===== | ===== How to start your session ===== | ||
| - | The system should be always on, you only need to: | + | If you are the **first user** of the day: |
| - | * Turn on the HXP lamp | + | * Turn off Components |
| - | * Turn on the computer (if needed) | + | * Turn off System/PC |
| + | * Turn off Main Switch | ||
| + | {{zeiss_lsm_880_1.jpg?100}} | ||
| + | * Turn on the computer | ||
| + | * Turn on Main Switch | ||
| + | * Turn on Systems/PC | ||
| + | * Turn on Components | ||
| + | * Turn on HXP Lamp | ||
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| - | * Start **ZEN** | + | * Start **ZEN Black** |
| + | Else: | ||
| + | * Log into Windows with your Agendo credentials | ||
| + | * Start **ZEN Black** | ||
| ===== How to end your session ===== | ===== How to end your session ===== | ||
| - | If there is another user for this microscope in the __next hour__: | + | If there is another user: |
| + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| * Exit the ZEN software | * Exit the ZEN software | ||
| - | * Leave the Ar laser on Standby | ||
| - | * Leave all other used lasers and HXP lamp on | ||
| * Log off the computer | * Log off the computer | ||
| + | If you are the **last user** of the day: | ||
| * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| - | Else: | + | * Turn off LASERs on ZEN |
| - | * Turn off the lasers on **ZEN** | + | |
| - | * Turn off the HXP lamp | + | |
| - | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | + | |
| - | * Exit the ZEN software | + | |
| - | * Log off the computer | + | |
| - | + | ||
| - | ===== Microscope Turn On Procedure (after complete shutdown) ===== | + | |
| - | + | ||
| - | If the system is completely off, please follow this procedure: | + | |
| - | + | ||
| - | {{zeiss_lsm_880_1.jpg?100 }} | + | |
| - | * Turn on the ''MAIN SWITCH'' | + | |
| - | * Turn on the ''SYSTEMS/PC'' switch | + | |
| - | * Turn on the ''COMPONENTS'' switch | + | |
| - | * Turn on the HXP lamp | + | |
| - | * Turn on the computer and login into Windows with your Agendo credentials | + | |
| - | * Start the **ZEN Black** software | + | |
| - | + | ||
| - | ===== Microscope Turn Off Procedure (for complete shutdown) ===== | + | |
| - | + | ||
| - | Please only shutdown the system if instructed to do so by the Bioimaging Staff. | + | |
| - | + | ||
| - | * Turn off the lasers on **ZEN** | + | |
| * Exit the ZEN software | * Exit the ZEN software | ||
| - | * Shutdown the computer | + | * Turn off HXP lamp |
| - | * Turn off the HXP lamp | + | * Turn off the computer |
| - | * Turn off the two switches ''SYSTEMS/PC'' and ''COMPONENTS'' | + | |
| - | * **Wait 5 min. for Ar laser to cool down** | + | |
| - | * Turn off the ''MAIN SWITCH'' (**do not turn the key**) | + | |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_lsm_880.1753713272.txt.gz · Last modified: 2025/07/28 16:34 by bioimaging
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