zeiss_lsm_880
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zeiss_lsm_880 [2024/07/15 20:39] bioimaging [Microscope Turn On Procedure (after complete shutdown)] |
zeiss_lsm_880 [2025/02/18 10:21] (current) bioimaging old revision restored (2025/01/14 16:37) |
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| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_name_new.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_zeiss_lsm_880_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_wiki_2022.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_wiki_2024.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_map_new.png">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_map_new.png">[Lisbon] Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: <a href="https://www.youtube.com/watch?v=sGTNBXq8omg">LSM 880 with Airyscan</a> (click for Zeiss webinar) <br> | <b>Model</b>: <a href="https://www.youtube.com/watch?v=sGTNBXq8omg">LSM 880 with Airyscan</a> (click for Zeiss webinar) <br> | ||
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|   <br> |   <br> | ||
| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
| - |   <br> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :confocal_pinhole.png?340}} The Zeiss LSM 880 with Airyscan is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] with 32 concentrically arranged GaAsP detection elements is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://www.youtube.com/watch?v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor 4x, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). | + | {{ :confocal_pinhole.png?340}} The Zeiss LSM 880 with Airyscan is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its Airyscan with 32 concentrically arranged GaAsP detection elements is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://www.youtube.com/watch?v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor 4x, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). |
| * [[https://www.youtube.com/watch?v=sGTNBXq8omg|ZEISS Webinar: LSM 880 with Airyscan - Revolutionize Your Confocal Imaging]] | * [[https://www.youtube.com/watch?v=sGTNBXq8omg|ZEISS Webinar: LSM 880 with Airyscan - Revolutionize Your Confocal Imaging]] | ||
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| </html> | </html> | ||
| - | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
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| ===== How to start your session ===== | ===== How to start your session ===== | ||
| - | The system should be always on, you only need to turn on the computer, log into Windows with your Agendo credentials and start **ZEN**. | + | The system should be always on, you only need to: |
| + | * Turn on the HXP lamp | ||
| + | * Turn on the computer | ||
| + | * Log into Windows with your Agendo credentials | ||
| + | * Start **ZEN** | ||
| ===== How to end your session ===== | ===== How to end your session ===== | ||
| If there is another user for this microscope in the __next hour__: | If there is another user for this microscope in the __next hour__: | ||
| - | * Exit the ZEN software, leave the lasers on | + | * Exit the ZEN software |
| + | * Leave the Ar laser on Standby | ||
| + | * Leave all other used lasers and HXP lamp on | ||
| * Log off the computer | * Log off the computer | ||
| * Clean up immersion objectives | * Clean up immersion objectives | ||
| Else: | Else: | ||
| * Turn off the lasers on **ZEN** | * Turn off the lasers on **ZEN** | ||
| + | * Turn off the HXP lamp | ||
| * Clean up immersion objectives | * Clean up immersion objectives | ||
| * Exit the ZEN software | * Exit the ZEN software | ||
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| ===== Microscope Turn On Procedure (after complete shutdown) ===== | ===== Microscope Turn On Procedure (after complete shutdown) ===== | ||
| - | If the system is completely off (e.g. during weekends), please follow this procedure: | + | If the system is completely off, please follow this procedure: |
| {{zeiss_lsm_880_1.jpg?100 }} | {{zeiss_lsm_880_1.jpg?100 }} | ||
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| * Turn on the ''SYSTEMS/PC'' switch | * Turn on the ''SYSTEMS/PC'' switch | ||
| * Turn on the ''COMPONENTS'' switch | * Turn on the ''COMPONENTS'' switch | ||
| - | * Turn on the computer | + | * Turn on the HXP lamp |
| - | * Login in to Windows with your Agendo credentials | + | * Turn on the computer and login into Windows with your Agendo credentials |
| * Start the **ZEN Black** software | * Start the **ZEN Black** software | ||
| ===== Microscope Turn Off Procedure (for complete shutdown) ===== | ===== Microscope Turn Off Procedure (for complete shutdown) ===== | ||
| + | |||
| + | Please only shutdown the system if instructed to do so by the Bioimaging Unit. | ||
| * Turn off the lasers on **ZEN** | * Turn off the lasers on **ZEN** | ||
| - | * Clean up immersion objectives | ||
| * Exit the ZEN software | * Exit the ZEN software | ||
| * Shutdown the computer | * Shutdown the computer | ||
| + | * Turn off the HXP lamp | ||
| * Turn off the two switches ''SYSTEMS/PC'' and ''COMPONENTS'' | * Turn off the two switches ''SYSTEMS/PC'' and ''COMPONENTS'' | ||
| * **Wait 5 min. for Ar laser to cool down** | * **Wait 5 min. for Ar laser to cool down** | ||
| - | * Turn off the ''MAIN SWITCH'' | + | * Turn off the ''MAIN SWITCH'' (**do not turn the key**) |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_lsm_880.1721068767.txt.gz · Last modified: 2024/07/15 20:39 by bioimaging
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