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bd_facs_symphony_a5_se [2023/12/15 17:53] flowcytometry |
bd_facs_symphony_a5_se [2024/03/23 21:50] (current) flowcytometry |
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<b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | <b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | ||
<b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | <b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | ||
- | <b>Model</b>: BD FACSymphony A5 SE</a><br> | + | <b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-facsymphony-a5-se" target="_blank">FACSymphony A5 SE</a><br> |
<b>Nickname</b>: "A5 SE"<br> | <b>Nickname</b>: "A5 SE"<br> | ||
<b>Software</b>: FACSDiva 9.6<br> | <b>Software</b>: FACSDiva 9.6<br> | ||
<b>Year</b>: 2023 <br> | <b>Year</b>: 2023 <br> | ||
- | <b>SN</b>: <br> | + | <b>SN</b>:R6649540104 <br> |
  <br> |   <br> | ||
  <br> |   <br> | ||
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→ <a href="/facility/flowcytometry/doku.php?id=bd_facsymphony_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png"> BD FACSymphony Usage Statistics</a> | → <a href="/facility/flowcytometry/doku.php?id=bd_facsymphony_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png"> BD FACSymphony Usage Statistics</a> | ||
<br> | <br> | ||
+ | → <a href="https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/BD-FACSymphony-A5-SE-Brochure1.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png"> BD FACSymphony A5 SE Brochure</a> | ||
</td> | </td> | ||
</tr> | </tr> | ||
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* Enables both spectral unmixing and compensation workflows | * Enables both spectral unmixing and compensation workflows | ||
\\ | \\ | ||
- | **If you need a high-throughput sampler, check the [[bd_lsr_fortessa_2|BD LSRFortessa 2]].** | + | **If you need high parameter cell analysis, check the [[cytek_aurora|CYTEK Aurora]].** |
- | \\ | + | |
- | **If you need a multicolor analysis of less then 16 parameters, check also [[bd_lsr_fortessa_1|BD LSRFortessa 1]], [[bd_lsr_fortessa_2|BD LSRFortessa 2]], [[bd_lsr_fortessa_X20|BD LSRFortessa X20]] or [[bd_accuri_c6|BD Accuri C6 Plus]] instead.** | + | **If you need a multicolor analysis of less then 16, 13 or 4 parameters, check also the [[bd_lsr_fortessa_X20|BD LSRFortessa X20]], the [[bd_lsr_fortessa_2|BD LSRFortessa]] or [[bd_accuri_c6|BD Accuri C6 Plus]].** |
+ | |||
+ | **If you need a high-throughput sampler, check the [[bd_lsr_fortessa_2|BD LSRFortessa]] or the [[cytek_aurora|CYTEK Aurora]].** | ||
\\ | \\ | ||
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===== Suggested Fluorochrome Peak Channels ===== | ===== Suggested Fluorochrome Peak Channels ===== | ||
- | {{{{:facsymphony_fluorophores.png?900|}} | + | {{{{:facsymphony_fluorophores.png|}} |
- | {{{{:facsymphony_fluorophores_2.png?900|}} | + | {{{{:facsymphony_fluorophores_2.png|}} |
===== Useful Links when Preparing your Experiment ===== | ===== Useful Links when Preparing your Experiment ===== | ||
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* [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.038_-_bd_facsymphony_a5_se_operation.pdf| BD FACSymphony A5 SE Operation]] | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.038_-_bd_facsymphony_a5_se_operation.pdf| BD FACSymphony A5 SE Operation]] | ||
- | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.022_-_bd_lsrfortessa_x-20_operation.pdf| BD FACSDiva Tutorial (for BD FACSymphony A5 SE)]] | + | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.037_-_bd_facsdiva_bd_facsymphony_a5_tutorial.pdf| BD FACSDiva Tutorial (for BD FACSymphony A5 SE)]] |
* [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.024_-_bd_lsrfortessa_facsymphony_a5_se_troubleshooting.pdf| BD LSRFortessa & FACSymphony A5 SE Troubleshooting]] | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.024_-_bd_lsrfortessa_facsymphony_a5_se_troubleshooting.pdf| BD LSRFortessa & FACSymphony A5 SE Troubleshooting]] | ||
* [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.040_-_bd_facsymphony_cleaning.pdf| BD FACSymphony A5 SE Cleaning]] | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.040_-_bd_facsymphony_cleaning.pdf| BD FACSymphony A5 SE Cleaning]] |