paneldesign
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paneldesign [2026/04/09 23:33] flowcytometry |
paneldesign [2026/04/16 19:15] (current) flowcytometry |
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| - | <h2 style="color:#2E75B6;">Panel Design Service</h2> | + | <h2 style="color:#2E75B6; margin-left:325px;">Panel Design Service</h2> |
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=panel_design_banner.jpg" | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=panel_design_banner.png" |
| - | style="width:300px; height:auto; float:left; margin-right:10px; margin-bottom:10px;"> | + | style="max-width:320px; width:100%; height:auto; float:left; margin-right:5px; margin-bottom:10px;"> |
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| By providing this support to researchers, we aim to significantly reduce troubleshooting, optimize experimental outcomes, and improve the overall quality of the data generated. | By providing this support to researchers, we aim to significantly reduce troubleshooting, optimize experimental outcomes, and improve the overall quality of the data generated. | ||
| - | If you need help designing a **New Panel** or improving an existing one, please complete sheets 1–4 of the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.flow.015_-_panel_design_1.xlsx" target="_blank">Panel Design Form</a></html> and send it to __flowcytometry@gimm.pt__. | + | If you need help designing a **New Panel** or improving an existing one, please complete sheets 1–4 of the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.flow.015_-_panel_design_1.xlsx" target="_blank">Panel Design Form</a></html> and send it to __flowcytometry@gimm.pt__ |
| Within the form, you will find the 8 steps required to design a new panel: | Within the form, you will find the 8 steps required to design a new panel: | ||
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| **1. Run an unstained sample under the experimental conditions you will use in your analysis** | **1. Run an unstained sample under the experimental conditions you will use in your analysis** | ||
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| This allows you to define the Autofluorescence (AF) spectrum of your cells so we can avoid fluorophores that fall on the same detectors. | This allows you to define the Autofluorescence (AF) spectrum of your cells so we can avoid fluorophores that fall on the same detectors. | ||
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| **2, 3. Design your Panel based on your biological question, animal model, tissue, and sample preparation conditions** | **2, 3. Design your Panel based on your biological question, animal model, tissue, and sample preparation conditions** | ||
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| Here you will define which markers to be used, their function, priority level, expression level, and co-express patterns. This information allows us to design your panel following Panel Design best practices and to provide you a tailored proposal. | Here you will define which markers to be used, their function, priority level, expression level, and co-express patterns. This information allows us to design your panel following Panel Design best practices and to provide you a tailored proposal. | ||
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| **4. Define the gating strategy for data analysis** | **4. Define the gating strategy for data analysis** | ||
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| **5. Evaluate similarity and Stain Index Reduction or Hotspot matrix** | **5. Evaluate similarity and Stain Index Reduction or Hotspot matrix** | ||
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| **6. Titrate all the antibodies to be used** | **6. Titrate all the antibodies to be used** | ||
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| + | After the titration of your antibodies, you are ready to do the settings on the instrument. Our team will be happy to support you during this session. | ||
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| - | After the titration of your antibodies, you are ready to do the settings on the instrument. Our team will be happy to support you during this session. | ||
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| When the settings on the instrument are done, we will: | When the settings on the instrument are done, we will: | ||
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| **7. Analyse the compensation/unmixing using a fully stained sample to validate the panel** | **7. Analyse the compensation/unmixing using a fully stained sample to validate the panel** | ||
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| **8. Troubleshoot the compensation/unmixing, if necessary** | **8. Troubleshoot the compensation/unmixing, if necessary** | ||
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| <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.flow.015_-_panel_design_1.xlsx" target="_blank">FORM.FLOW.015 - Panel Design</a></html> | <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.flow.015_-_panel_design_1.xlsx" target="_blank">FORM.FLOW.015 - Panel Design</a></html> | ||
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| - | <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.994_-_good_practices_-_setting_and_sample_preparation.pdf" target="_blank"> SOP.FLOW.994 - Good Practices - Settings and Sample Preparation</a></html> | + | <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.994_-_good_practices_unstained_and_single_color_controls_preparation.pdf" target="_blank"> SOP.FLOW.994 - Good Practices - Unstained and Single Color Controls Preparation</a></html> |
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| <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.995_-_compensation_unmixing_troubleshooting.pdf" target="_blank"> SOP.FLOW.995 - Compensation and Unmixing Troubleshooting</a></html> | <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.995_-_compensation_unmixing_troubleshooting.pdf" target="_blank"> SOP.FLOW.995 - Compensation and Unmixing Troubleshooting</a></html> | ||
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| - | [[newuser|Back to New User - Access to our resources]] | + | [[supportservices|Back to Support Services]] |
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| + | [[start|Back to Flow Cytometry Wiki]] | ||
paneldesign.1775770409.txt.gz · Last modified: 2026/04/09 23:33 by flowcytometry
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