paneldesign
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paneldesign [2026/03/30 14:54] flowcytometry |
paneldesign [2026/03/30 18:52] (current) flowcytometry |
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| Our Panel Design Service includes one-on-one consultations, tailored panel recommendations, and guidance on fluorophore selection based on antigen density, co-expression profiles, reagents available, and instrument compatibility. | Our Panel Design Service includes one-on-one consultations, tailored panel recommendations, and guidance on fluorophore selection based on antigen density, co-expression profiles, reagents available, and instrument compatibility. | ||
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| - | By providing this support to researchers, we aim to significantly reduce reduce troubleshooting, optimize experimental outcomes, and improve the overall quality of the data generated. | + | By providing this support to researchers, we aim to significantly reduce troubleshooting, optimize experimental outcomes, and improve the overall quality of the data generated. |
| - | If you need help designing a **new Panel** or improving an existing one, please complete sheets 1–4 of the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.flow.015_-_panel_design_1.xlsx" target="_blank">Panel Design Form</a></html> and send it to flowcytometry@gimm.pt. | + | If you need help designing a **New Panel** or improving an existing one, please complete sheets 1–4 of the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.flow.015_-_panel_design_1.xlsx" target="_blank">Panel Design Form</a></html> and send it to __flowcytometry@gimm.pt__. |
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| **2, 3. Design your Panel based on your biological question, animal model, tissue, and sample preparation conditions** | **2, 3. Design your Panel based on your biological question, animal model, tissue, and sample preparation conditions** | ||
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| - | Here you will define which markers to be used, their function, priority level, expression level, and co-express patterns. This information allows us to design your panel following best practices (see SOP link) and provide a tailored proposal. | + | Here you will define which markers to be used, their function, priority level, expression level, and co-express patterns. This information allows us to design your panel following Panel Design best practices and to provide you a tailored proposal. |
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| - | **4. Define the gating strategy for Data Analysis** | + | **4. Define the gating strategy for data analysis** |
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| **5. Evaluate similarity and Stain Index Reduction or Hotspot matrix** | **5. Evaluate similarity and Stain Index Reduction or Hotspot matrix** | ||
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| **6. Titrate all the antibodies to be used** | **6. Titrate all the antibodies to be used** | ||
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| - | After the titration of your antibodies, you are ready to do the settings on the instrument (SOP link). Our team will be happy to support you during this session. | + | \\ |
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| + | After the titration of your antibodies, you are ready to do the settings on the instrument. Our team will be happy to support you during this session. | ||
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| When the settings on the instrument are done, we will: | When the settings on the instrument are done, we will: | ||
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| **7. Analyse the compensation/unmixing using a fully stained sample to validate the panel** | **7. Analyse the compensation/unmixing using a fully stained sample to validate the panel** | ||
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| - | **8. Troubleshoot the compensation/unmixing if necessary** (SOP link) | + | **8. Troubleshoot the compensation/unmixing, if necessary** |
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| + | ====Important links:==== | ||
| + | <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.flow.015_-_panel_design_1.xlsx" target="_blank">FORM.FLOW.015 - Panel Design</a></html> | ||
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| + | <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.994_-_good_practices_-_setting_and_sample_preparation.pdf" target="_blank"> SOP.FLOW.994 - Good Practices - Settings and Sample Preparation</a></html> | ||
| + | \\ | ||
| + | <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.995_-_compensation_unmixing_troubleshooting.pdf" target="_blank"> SOP.FLOW.995 - Compensation and Unmixing Troubleshooting</a></html> | ||
| + | <html><a href="https://www.bdbiosciences.com/en-us/products/software/bd-research-cloud" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html> | ||
| + | <html><a href="https://cloud.cytekbio.com/login" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=cytek_cloud.png" width=300></a></html> | ||
| - | **Important information:** | ||
| - | * <html><a href="https://www.flowjo.com/solutions/flowjo/downloads/tutorials" target="_blank"> FlowJo Tutorials</a></html> | ||
| - | * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=flowjo_basic_tutorial_2018.pdf" target="_blank"> FlowJo Basic Tutorial </a></html> | ||
| - | * <html><a href="https://denovosoftware.com/" target="_blank">FCS Express Software</a></html> | ||
| - | * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.009_-_modfit_lt_tutorial.pdf" target="_blank">ModFit Tutorial</a></html> | ||
| - | * <html><a href="https://www.medizin.uni-tuebingen.de/files/download/8YWbqX1VelmV0EKRLxkr9apM/IDEAS-User-Manual_6.2.pdf" target="_blank">IDEAS Software User Manual</a></html> | ||
| - | * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.027_-_ideas_software_tutorial.pdf" target="_blank">IDEAS Tutorial</a></html> | ||
| - | * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf" target="_blank">User Guidelines</a></html> | ||
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| [[newuser|Back to New User - Access to our resources]] | [[newuser|Back to New User - Access to our resources]] | ||
paneldesign.1774875249.txt.gz · Last modified: 2026/03/30 14:54 by flowcytometry
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