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imagestreamx [2025/12/19 18:18] flowcytometry |
imagestreamx [2026/07/20 19:25] (current) flowcytometry [Objectives Information] |
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| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=amnis_nome_gimm.png"> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=amnis_nome_gimm.png" WIDTH=680> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_wiki.png"WIDTH=300> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=amnis_new.png"WIDTH=300> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| <b>Location</b>: Oeiras Site, Room 0B01, Bartolomeu Dias Wing (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 4625) <br> | <b>Location</b>: Oeiras Site, Room 0B01, Bartolomeu Dias Wing (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 4625) <br> | ||
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| * **Multimag**: 20x, 40x and 60x magnification | * **Multimag**: 20x, 40x and 60x magnification | ||
| * **Extended depth of field**: projects all structures within a cell into a single plane of focus | * **Extended depth of field**: projects all structures within a cell into a single plane of focus | ||
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| + | The **Cytek AMNIS ImageStream X** is equipped with an autosampler, which allows the automated acquisition of samples and is compatible with 96-well U and V plates. | ||
| <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=img-12channels.jpg"> | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=img-12channels.jpg"> | ||
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| ^ Magnification ^ 20x ^ 40x ^ 60x ^ | ^ Magnification ^ 20x ^ 40x ^ 60x ^ | ||
| ^ Numerical Aperture | 0.50 | 0.75 | 0.90 | | ^ Numerical Aperture | 0.50 | 0.75 | 0.90 | | ||
| - | ^ Field of View (μm) | 120 | 60 | 40 | | + | ^ Field of View (μm) | 120 x 256 | 60 x 128 | 40 x 170 | |
| - | ^ Pixel Size (μm) | 1.0 | 0.5 | 0.33 | | + | ^ Pixel Size (μm) | 1.0 x 1.0 | 0.5 x 0.5 | 0.3 x 0.3 | |
| + | ^ Imaging rate (Obj/sec) | 5000 | 2000 | 1200 | | ||
| ^ Depth of Field (μm) | 8.0 | 4.0 | 2.5 | | ^ Depth of Field (μm) | 8.0 | 4.0 | 2.5 | | ||
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| - | [[resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |
| + | \\ | ||
| + | [[start|Back to the Flow Cytometry Wiki]] | ||