imagestreamx
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imagestreamx [2024/01/19 14:18] flowcytometry |
imagestreamx [2024/08/26 17:31] (current) flowcytometry |
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| <html> | <html> | ||
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=amnis_nome.png"> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=amnis_nome_gimm.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_wiki.png"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_wiki.png" width=300></a> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_wiki.png"WIDTH=300> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | + | <b>Location</b>: Lisbon Site, Room P2-A-49 (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> |
| <b>Manufacturer</b>: <a href="https://cytekbio.com/" target="_blank">Cytek</a><br> | <b>Manufacturer</b>: <a href="https://cytekbio.com/" target="_blank">Cytek</a><br> | ||
| <b>Model</b>: <a href="https://cytekbio.com/pages/imagestream" target="_blank">Amnis ImageStreamX Mark II</a><br> | <b>Model</b>: <a href="https://cytekbio.com/pages/imagestream" target="_blank">Amnis ImageStreamX Mark II</a><br> | ||
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|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://my.agendoscience.com/calendar/?id=MTBjYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png"> Amnis ImageStreamX Booking</a> | + | → <a href="https://europe.agendoscience.com/calendar/?id=MTBjYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png"> Cytek Amnis ImageStreamX Booking</a> |
| <br> | <br> | ||
| - | → <a href="/facility/flowcytometry/doku.php?id=amnis_imagestreamx_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png"> Amnis ImageStreamX Usage Statistics</a> | + | → <a href="/facility/flowcytometry/doku.php?id=amnis_imagestreamx_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png"> Cytek Amnis ImageStreamX Usage Statistics</a> |
| <br> | <br> | ||
| - | → <a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=amnis_brochure.pdf"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png"> Amnis ImageStream Brochure</a> | + | → <a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=amnis_brochure.pdf"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png"> Cytek Amnis ImageStream Brochure</a> |
|   </p> |   </p> | ||
| </td> | </td> | ||
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| ===== System overview ===== | ===== System overview ===== | ||
| - | {{ :how_the_imagestream_works.png?0x300|}} The Amnis ImageStreamX is a benchtop multispectral //imaging flow cytometer// equipped with two CCD cameras that can acquire up to 12 images simultaneously of each cell or object in brightfield, scatter and multiple fluorescent channels at rates up to 5000 objects/sec. It has five lasers - 375 nm, 488 nm, 561 nm, 642 nm and 785 nm (dedicated to side scatter). Cells in suspension are hydrodynamically focused in a flow cell, where they are illuminated by a brightfield light source and by lasers. High numerical aperture (NA) objective lenses collect fluorescence emission, as well as scattered and transmitted light from the cells which then intersect a spectral decomposition element that focus different spectral bands on different physical locations of two CCD cameras. By collecting large numbers of digital images per sample and providing numerical representation of image-based features, the ImageStreamX combines single cell standard microscopy information with the statistical significance of large sample sizes in standard flow cytometry. While you can use it to measure fluorescence intensity as in conventional flow cytometers, the best applications for the ImageStreamX take advantage of its imaging abilities to locate and quantify the distribution of signals within cells or even between cells in cell conjugates. The system also has a dedicated software application used for spectral compensation, image analysis and statistical analysis of acquired images (IDEAS) with predefined wizards for apoptosis, cell cycle, co-localization, internalization, shape change, spot counting and others. | + | {{ :how_the_imagestream_works.png?0x300|}} The Cytek Amnis ImageStreamX is a benchtop multispectral //imaging flow cytometer// equipped with two CCD cameras that can acquire up to 12 images simultaneously of each cell or object in brightfield, scatter and multiple fluorescent channels at rates up to 5000 objects/sec. It has five lasers - 375 nm, 488 nm, 561 nm, 642 nm and 785 nm (dedicated to side scatter). Cells in suspension are hydrodynamically focused in a flow cell, where they are illuminated by a brightfield light source and by lasers. High numerical aperture (NA) objective lenses collect fluorescence emission, as well as scattered and transmitted light from the cells which then intersect a spectral decomposition element that focus different spectral bands on different physical locations of two CCD cameras. By collecting large numbers of digital images per sample and providing numerical representation of image-based features, the ImageStreamX combines single cell standard microscopy information with the statistical significance of large sample sizes in standard flow cytometry. While you can use it to measure fluorescence intensity as in conventional flow cytometers, the best applications for the ImageStreamX take advantage of its imaging abilities to locate and quantify the distribution of signals within cells or even between cells in cell conjugates. The system also has a dedicated software application used for spectral compensation, image analysis and statistical analysis of acquired images (IDEAS) with predefined wizards for apoptosis, cell cycle, co-localization, internalization, shape change, spot counting and others. |
| * **12 imaging channels** (2 brightfield channels + 10 channels for fluorescence detection) | * **12 imaging channels** (2 brightfield channels + 10 channels for fluorescence detection) | ||
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| * **Extended depth of field**: projects all structures within a cell into a single plane of focus | * **Extended depth of field**: projects all structures within a cell into a single plane of focus | ||
| - | {{ ::img-12channels.jpg |}} | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=img-12channels.jpg"> |
| + | </html> | ||
| ===== Configuration ===== | ===== Configuration ===== | ||
| - | {{::imagestreamx_fluorochrome_chart.png?700|}} | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_fluorochrome_chart.png"> |
| + | </html> | ||
| ===== Objectives Information ===== | ===== Objectives Information ===== | ||
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| ===== Image Acquisition Performance ===== | ===== Image Acquisition Performance ===== | ||
| - | {{::high_speed_imaging.png?600|}} | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=high_speed_imaging.png"WIDTH=600> |
| + | </html> | ||
| ===== Additional information ===== | ===== Additional information ===== | ||
| - | * **[[prepare_samples|How to prepare samples for Flow Cytometry]]** | + | <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=how_to_prepare_samples_for_flow_cytometry.png" WIDTH=400></a></html> |
| - | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.026_-_amnis_imagestreamx_operation.pdf| Amnis ImageStreamX Operation]] | + | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.026_-_amnis_imagestreamx_operation.pdf| Cytek Amnis ImageStreamX Operation]] |
| * {{::imagestreamx_sample_preparation_guide.pdf| Sample Preparation Guide}}. **Note**: Samples are run using 1.5 ml microfuge tubes (Sarstedt ref. 72706) | * {{::imagestreamx_sample_preparation_guide.pdf| Sample Preparation Guide}}. **Note**: Samples are run using 1.5 ml microfuge tubes (Sarstedt ref. 72706) | ||
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
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| Available 7 days a week, 24 hours a day for trained researchers. | Available 7 days a week, 24 hours a day for trained researchers. | ||
| - | Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | + | Please check the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines_.pdf" target="_blank"> User Guidelines</a></html> for detailed information. |
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
imagestreamx.1705670323.txt.gz · Last modified: 2024/01/19 14:18 by flowcytometry
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