bd_lsr_fortessa_x20_oeiras
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bd_lsr_fortessa_x20_oeiras [2024/08/22 18:12] flowcytometry created |
bd_lsr_fortessa_x20_oeiras [2025/02/14 19:31] (current) flowcytometry |
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| - | <a href="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_wiki_2.jpg"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_wiki_2.jpg" width=300></a> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=x20_oeiras_wiki.png"WIDTH=300> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: Oeiras site <br> | + | <b>Location</b>: Oeiras Site, Room 0B01, Bartolomeu Dias Wing,<br>(<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 4625)<br> |
| <b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | <b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | ||
| <b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-lsrfortessa-x-20" target="_blank">LSRFortessa X-20</a><br> | <b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-lsrfortessa-x-20" target="_blank">LSRFortessa X-20</a><br> | ||
| - | <b>Nickname</b>: "X20"<br> | + | <b>Nickname</b>: "X20 Oeiras"<br> |
| - | <b>Software</b>: FACSDiva 8.0<br> | + | <b>Software</b>: BD FACSDiva 9.0<br> |
| - | <b>Year</b>: 2017 <br> | + | <b>Year</b>: 2016 <br> |
| - | <b>SN</b>: H65767SR1029 <br> | + | <b>SN</b>: H65767502017 <br> |
|   <br> |   <br> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://europe.agendoscience.com/calendar/?id=NDJjYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png"> BD LSRFortessa X-20 Booking</a> | + | → <a href="https://europe.agendoscience.com/calendar/?id=MTQ1Y2FsZW5kYXJz" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png"> BD LSRFortessa X-20 Booking</a> |
| <br> | <br> | ||
| → <a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsr_fortessax20_brochure.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png"> BD LSRFortessa X-20 Brochure</a> | → <a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsr_fortessax20_brochure.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png"> BD LSRFortessa X-20 Brochure</a> | ||
| Line 23: | Line 23: | ||
| </table> | </table> | ||
| </html> | </html> | ||
| - | {{:info.gif|}} **Next Scheduled Maintenances**: | + | {{:info.gif|}} **Next Scheduled Maintenances**: 18 March and 16 September |
| ===== System overview ===== | ===== System overview ===== | ||
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| * Four lasers with violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (640 nm) | * Four lasers with violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (640 nm) | ||
| \\ | \\ | ||
| + | If you need multicolor analysis of more than 16 parameters, check the [[bd_facs_symphony_a5_se|BD FACSymphony A5 SE]] or the [[cytek_aurora|CYTEK Aurora]] in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html> and the [[cytek_aurora_oeiras|CYTEK Aurora]] in <html><span style="color:yellowgreen;font-size:100%;">Oeiras site</span></html>. | ||
| + | If you need a high-throughput sampler, check the [[bd_lsr_fortessa_2|BD LSRFortessa]] or the [[cytek_aurora|CYTEK Aurora]] in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html> and the [[cytek_aurora_oeiras|CYTEK Aurora]] or the [[bd_lsr_fortessa_sorp|BD LSRFortessa SORP]] in <html><span style="color:yellowgreen;font-size:100%;">Oeiras site</span></html>. | ||
| ===== Configuration ===== | ===== Configuration ===== | ||
| - | {{:bd_lsrfortessa_x-20_configuration_new_.png?900|}} | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_x-20_configuration_new_.png"WIDTH=900></html> |
| - | \\ | + | |
| \\ | \\ | ||
| ===== Fluorophores Compatibility ===== | ===== Fluorophores Compatibility ===== | ||
| - | {{{{:fortessa_x20_fluorochromes.png?900|}} | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_fluorochromes.png"WIDTH=900><br><br> |
| - | \\ | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_additional_filters.png"WIDTH=250><br><br> |
| - | {{:fortessa_x20_additional_filters.png?250|}} | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_x-20_405.png"WIDTH=700><br> |
| - | \\ | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_x-20_488.png"WIDTH=700><br> |
| - | \\ | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_x-20_561.png"WIDTH=700><br> |
| - | {{::bd_lsrfortessa_x-20_405.png?700|}} | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_x-20_640.png"WIDTH=700> |
| - | \\ | + | </html> |
| - | {{::bd_lsrfortessa_x-20_488.png?700|}} | + | |
| - | \\ | + | |
| - | {{::bd_lsrfortessa_x-20_561.png?700|}} | + | |
| - | \\ | + | |
| - | {{::bd_lsrfortessa_x-20_640.png?700|}} | + | |
| ===== Useful Links when Preparing your Experiment ===== | ===== Useful Links when Preparing your Experiment ===== | ||
| - | * [[prepare_samples|{{how_to_prepare_samples_for_flow_cytometry.png}}]] | + | * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=how_to_prepare_samples_for_flow_cytometry.png" WIDTH=400></a></html> |
| * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html> | * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html> | ||
| * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html> | * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html> | ||
| - | |||
| - | ===== Additional Information ===== | ||
| - | |||
| - | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.022_-_bd_lsrfortessa_x-20_operation.pdf| BD LSRFortessa X-20 Operation]] | ||
| - | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.003_-_bd_facsdiva_tutorial.pdf| BD FACSDiva Software Tutorial]] | ||
| - | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.024_-_bd_lsrfortessa_troubleshooting.pdf| BD LSRFortessa Troubleshooting]] | ||
| - | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.025_-_bd_lsrfortessa_cleaning.pdf| BD LSRFortessa Cleaning]] | ||
| - | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.030_-_bd_lsrfortessa_X20_starting_up_cleaning.pdf| BD LSRFortessa X20 Starting up Cleaning]] for the first user of the day, on weekends and out of staff working hours | ||
| - | |||
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| Available 7 days a week, 24 hours a day for trained researchers. | Available 7 days a week, 24 hours a day for trained researchers. | ||
| - | |||
| - | Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
bd_lsr_fortessa_x20_oeiras.1724343162.txt.gz · Last modified: 2024/08/22 18:12 by flowcytometry
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