bd_facs_symphony_a5_se
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bd_facs_symphony_a5_se [2024/08/23 15:16] flowcytometry |
bd_facs_symphony_a5_se [2025/04/15 17:20] (current) flowcytometry |
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| <img src="/facility/flowcytometry/lib/exe/fetch.php?media=facsymphony_wiki_page.jpg"WIDTH=300> | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=facsymphony_wiki_page.jpg"WIDTH=300> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: Lisbon site: Room P2-A-49 (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | + | <b>Location</b>: Lisbon Site, Room P2-A-49 (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> |
| <b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | <b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | ||
| <b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-facsymphony-a5-se" target="_blank">FACSymphony A5 SE</a><br> | <b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-facsymphony-a5-se" target="_blank">FACSymphony A5 SE</a><br> | ||
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| </table> | </table> | ||
| </html> | </html> | ||
| - | {{:info.gif|}} **Next Scheduled Maintenances**: | + | {{:info.gif|}} **Next Scheduled Maintenances**:14 April |
| ===== System overview ===== | ===== System overview ===== | ||
| {{ :flow_cell_sit_sample.png?0x300|}} | {{ :flow_cell_sit_sample.png?0x300|}} | ||
| {{ :facsymphony_detectors.png?0x200|}} The BD FACSymphony™ A5 SE allows for high-parameter spectral flow cytometry. It enables you to gain full visible spectrum coverage to collect all light emitted without the need for filter changes, increasing dye flexibility and number of simultaneous colors. It also allows for autofluorescence unmixing, that may enable the resolution of difficult cells and populations. | {{ :facsymphony_detectors.png?0x200|}} The BD FACSymphony™ A5 SE allows for high-parameter spectral flow cytometry. It enables you to gain full visible spectrum coverage to collect all light emitted without the need for filter changes, increasing dye flexibility and number of simultaneous colors. It also allows for autofluorescence unmixing, that may enable the resolution of difficult cells and populations. | ||
| + | \\ | ||
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| * Enables both spectral unmixing and compensation workflows | * Enables both spectral unmixing and compensation workflows | ||
| \\ | \\ | ||
| - | **If you need high parameter cell analysis, check the [[cytek_aurora|CYTEK Aurora]].** | + | \\ |
| - | + | If you need high parameter cell analysis and a high-throughput sampler, check also the [[cytek_aurora|CYTEK Aurora]] in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html> and the [[cytek_aurora_oeiras|CYTEK Aurora]] in <html><span style="color:yellowgreen;font-size:100%;">Oeiras site</span></html>. | |
| - | **If you need a multicolor analysis of less then 16, 13 or 4 parameters, check also the [[bd_lsr_fortessa_X20|BD LSRFortessa X20]], the [[bd_lsr_fortessa_2|BD LSRFortessa]] or [[bd_accuri_c6|BD Accuri C6 Plus]].** | + | |
| - | + | ||
| - | **If you need a high-throughput sampler, check the [[bd_lsr_fortessa_2|BD LSRFortessa]] or the [[cytek_aurora|CYTEK Aurora]].** | + | |
| \\ | \\ | ||
| ===== Configuration ===== | ===== Configuration ===== | ||
| - | {{:facsymphony_optical_configuration.png?900|}} | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=facsymphony_optical_configuration.png"WIDTH=900></html> |
| \\ | \\ | ||
| \\ | \\ | ||
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| ===== Suggested Fluorochrome Peak Channels ===== | ===== Suggested Fluorochrome Peak Channels ===== | ||
| - | {{{{:facsymphony_fluorophores.png|}} | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=facsymphony_fluorophores.png"><br> |
| - | {{{{:facsymphony_fluorophores_2.png|}} | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=facsymphony_fluorophores_2.png"> |
| + | </html> | ||
| ===== Useful Links when Preparing your Experiment ===== | ===== Useful Links when Preparing your Experiment ===== | ||
| - | * [[prepare_samples|{{how_to_prepare_samples_for_flow_cytometry.png}}]] | + | * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=how_to_prepare_samples_for_flow_cytometry.png" WIDTH=400></a></html> |
| * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html> | * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html> | ||
| * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html> | * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html> | ||
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| Available 7 days a week, 24 hours a day for trained researchers. | Available 7 days a week, 24 hours a day for trained researchers. | ||
| - | Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | + | Please check the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines_.pdf" target="_blank"> User Guidelines</a></html> for detailed information. |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
bd_facs_symphony_a5_se.1724419018.txt.gz · Last modified: 2024/08/23 15:16 by flowcytometry
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