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zeiss_lsm_880 [2021/09/15 18:00] bioimaging [Objectives] |
zeiss_lsm_880 [2022/01/20 13:34] bioimaging [Microscope Turn On Procedure] |
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| 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | ||
| 40x | Plan-Apochromat Corr | Dry | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | | 40x | Plan-Apochromat Corr | Dry | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | ||
- | | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | | ||
==== Filtersets (Ocular) ==== | ==== Filtersets (Ocular) ==== | ||
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* Turn on the ''MAIN SWITCH'' | * Turn on the ''MAIN SWITCH'' | ||
{{zeiss_lsm_880_1.jpg?100}} | {{zeiss_lsm_880_1.jpg?100}} | ||
- | * Turn on the incubation system (**A** on remote control) | ||
* Turn on the ''SYSTEMS/PC'' switch | * Turn on the ''SYSTEMS/PC'' switch | ||
* Turn on the computer | * Turn on the computer | ||
* Login in to Windows (Bioimaging User) | * Login in to Windows (Bioimaging User) | ||
* Turn on the ''COMPONENTS'' switch | * Turn on the ''COMPONENTS'' switch | ||
- | * Check that the computer is connected to an Unidentified network (click on the network icon in the taskbar) | ||
* Start the **ZEN Black** software | * Start the **ZEN Black** software | ||
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- | **Warning**: If you need to change the stage adapter, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Unit</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47222</html>) | ||
===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
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* Shutdown the computer | * Shutdown the computer | ||
* Turn off the two switches ''SYSTEMS/PC'' and ''COMPONENTS'' | * Turn off the two switches ''SYSTEMS/PC'' and ''COMPONENTS'' | ||
- | * Turn off the incubation system (**A** on remote control) | ||
* **Wait 5 min. for Ar laser to cool down** | * **Wait 5 min. for Ar laser to cool down** | ||
* Turn off the ''MAIN SWITCH'' | * Turn off the ''MAIN SWITCH'' |