zeiss_lsm_880
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zeiss_lsm_880 [2020/01/14 18:06] bioimaging [Microscope Turn On Procedure] |
zeiss_lsm_880 [2020/01/17 12:36] bioimaging |
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| - Users must indicate which lasers they are going to use in the booking Description field | - Users must indicate which lasers they are going to use in the booking Description field | ||
| - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) | - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) | ||
| + | |||
| ===== System components ===== | ===== System components ===== | ||
| Line 67: | Line 68: | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | + | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | |
| | 40x | Plan-Apochromat Corr | Dry | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | | 40x | Plan-Apochromat Corr | Dry | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | ||
| | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | | | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | | ||
| Line 88: | Line 89: | ||
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| - | If there's another user for this microscope in the next hour: | + | If there is another user for this microscope in the __next hour__: |
| - | * Close the software, leave the lasers on and log off the computer. | + | * Exit the ZEN software, leave the lasers on |
| - | * Clean up any objective where you used immersion oil. | + | * Log off the computer |
| - | * **Make sure that there really is another user going to use the microscope.** | + | * Clean up immersion objectives |
| Else: | Else: | ||
| * Turn off the lasers on **ZEN** | * Turn off the lasers on **ZEN** | ||
| - | * Clean up any objective were you used immersion oil. | + | * Clean up immersion objectives |
| - | * Exit the software, shut down the computer. | + | * Exit the ZEN software |
| - | * Turn off the main switches ''SYSTEMS/PC'' and ''COMPONENTS'' | + | * Shutdown the computer |
| - | * Wait 5 min. for Ar laser cooldown | + | * Turn off the two switches ''SYSTEMS/PC'' and ''COMPONENTS'' |
| - | * Turn off the ''MAIN SWITCH''. | + | * **Wait 5 min. for Ar laser to cool down** |
| + | * Turn off the ''MAIN SWITCH'' | ||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
zeiss_lsm_880.txt · Last modified: 2024/01/28 11:47 by bioimaging
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