zeiss_cell_observer_sd
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zeiss_cell_observer_sd [2022/09/02 12:56] bioimaging |
zeiss_cell_observer_sd [2024/04/01 23:53] (current) bioimaging |
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| + | Data will be deleted after: <b>1 month</b> | ||
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| ===== System overview ===== | ===== System overview ===== | ||
| - | {{ :yokogawa_sd.png?350|}} The Zeiss Cell Observer SD //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control, a stage top incubator for CO2 supply and with Definite Focus.2 for hardware focus control during large tile or multi-position/time-lapse acquisitions (check tutorial videos below). You can also use the system as a widefield microscope, using fast-swicthing LEDs for illumination (Zeiss Colibri.2) and a sCMOS camera for acquisition. Even though its resolution is not as high as a //point-scanning confocal system// such as the [[zeiss_lsm_710|Zeiss LSM 710]], it is much faster. You can also check the [[3i_marianas_sdc|3i Marianas SDC]] if you need a spinning disk with higher laser power illumination. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]]. | + | {{ :yokogawa_sd.png?350|}} The Zeiss Cell Observer SD //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control, a stage top incubator for CO2 supply and with Definite Focus.2 for hardware focus control during large tile or multi-position/time-lapse acquisitions (check tutorial videos below). You can also use the system as a widefield microscope, using fast-swicthing LEDs for illumination (Zeiss Colibri.2) and a sCMOS camera for acquisition. |
| - | * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | + | * **Microscope**: [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research.html|Zeiss Axio Observer]] |
| - | * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-platforms/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit/|Yokogawa CSU-x1]] | + | * **LED Illumination**: [[https://www.zeiss.com/microscopy/en/products/accessories/colibri.html|Zeiss Colibri 5]] |
| + | * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-and-services/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit//|Yokogawa CSU-X1]] | ||
| * **EMCCD Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Evolve 512 EMCCD]] | * **EMCCD Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Evolve 512 EMCCD]] | ||
| * **sCMOS Camera**: [[https://www.hamamatsu.com/content/dam/hamamatsu-photonics/sites/documents/99_SALES_LIBRARY/sys/SCAS0156E_HCImage-Flash4.0V2.pdf|Hamamatsu ORCA-flash4.0 V2]] | * **sCMOS Camera**: [[https://www.hamamatsu.com/content/dam/hamamatsu-photonics/sites/documents/99_SALES_LIBRARY/sys/SCAS0156E_HCImage-Flash4.0V2.pdf|Hamamatsu ORCA-flash4.0 V2]] | ||
| - | * [[https://www.zeiss.com/microscopy/int/products/light-microscopes/axio-observer-for-biology/definite-focus.html|Definite Focus.2]] | + | * Definite Focus.2 |
| * | [[https://www.youtube.com/watch?v=Che0bouWcVE|Introduction to Definite Focus.2]] | * | [[https://www.youtube.com/watch?v=Che0bouWcVE|Introduction to Definite Focus.2]] | ||
| * | [[https://www.youtube.com/watch?v=I_c3SAHF984|Perform Large Tile Experiments with Definite Focus.2]] | * | [[https://www.youtube.com/watch?v=I_c3SAHF984|Perform Large Tile Experiments with Definite Focus.2]] | ||
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| ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ Mode ^ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ Mode ^ | ||
| | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | Confocal | | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | Confocal | | ||
| - | | [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | Widefield | | + | | [[https://www.hamamatsu.com/content/dam/hamamatsu-photonics/sites/documents/99_SALES_LIBRARY/sys/SCAS0156E_HCImage-Flash4.0V2.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | Widefield | |
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
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| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| If there is another user for this microscope in the __next hour__: | If there is another user for this microscope in the __next hour__: | ||
| - | * Exit the **ZEN** software, leave the lasers on | + | * Exit the **ZEN** software |
| * Log off the computer | * Log off the computer | ||
| * Clean up immersion objectives | * Clean up immersion objectives | ||
zeiss_cell_observer_sd.1662116168.txt.gz · Last modified: 2022/09/02 12:56 by bioimaging
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