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Table of Contents
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Location: Room P2-A-22 ( |
Zeiss Cell Observer SD BookingSystem overview
The Zeiss Cell Observer SD spinning disk confocal microscope is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512×512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control, a small stage incubator for CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. You can also use the system as a widefield microscope, using a sCMOS camera for acquisition.
- Microscope: Zeiss Axio Observer
- Confocal scanner: Yokogawa CSU-x1
- EMCCD Camera: Evolve 512 EMCCD
- sCMOS Camera: Hamamatsu ORCA-flash4.0 V2
System components
LASERs
| Laser Unit | Wavelength | Maximum Power | Current Status |
|---|---|---|---|
| Solid State Laser 405 | 405 nm | 50 mW | ok |
| Solid State Laser 488 | 488 nm | 100 mW | ok |
| Solid State Laser 561 | 561 nm | 75 mW | ok |
| Solid State Laser 638 | 638 nm | 75 mW | ok |
Objectives
| Magnification | Model | Type | NA | WD (mm) |
|---|---|---|---|---|
| 20x | Plan-Apochromat | Dry | 0.8 | 0.55 |
| 40x | LD C-Apochromat | Water | 1.1 | 0.62 |
| 40x | Plan-Apochromat | Oil | 1.4 | 0.13 |
| 63x | Plan-Apochromat | Oil | 1.40 | 0.19 |
| 100x | Plan-Apochromat | Oil | 1.40 | 0.17 |
Upon request:
| Magnification | Model | Type | NA | WD (mm) |
|---|---|---|---|---|
| 25x | LCI Plan-NeoFluar | Oil/Glyc/W | 0.8 | 0.21 |
| 40x | Plan-Apochromat | Dry | 0.95 | 0.25 |
Emission Filtersets
| Setting | Transmission |
|---|---|
| 1: 445 | 422-477 nm |
| 2: 525 | 510-540 nm |
| 3: 617 | 580-653 nm |
| 4: 692 | 672-712 nm |
| 5: QUAD | 440+521+607+700 nm |
| 6: Block | No Transmission |
| 7: Block | No Transmission |
| 8: Block | No Transmission |
| 9: Empty | Empty |
| 10: 445 | 422-477 nm |
Microscope Turn On Procedure
- Check that the main power supply switch is on (should be on by default)
- Turn on the fluorescence lamp (if needed)
- Turn on the secondary power supply switch
- Turn on the power switch distributor (green) and the component power switches (red) one at a time
- Turn on the Yokogawa spinning disk scanner (using the key)
- Turn on incubation and CO2 on the touchscreen (if needed)
- Turn on the lasers you need to use (using the key and the power switches)
- Turn on the computer
- Wait 2 min to allow for system components detection
- Start the SlideBook software
Warning: If you need to change the stage adaptor, please contact the Bioimaging Facility (imm-bioimaging@medicina.ulisboa.pt | 
47316)
Microscope Turn Off Procedure
- Remove your sample from the stage/piezo holder
- Exit the SlideBook software
- Switch off the computer
- Turn off all system components (follow the Turn On Procedure in reverse)
