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Microscope overview
The Andor Spinning W1 system is a spinning disk laser confocal microscope with a sensitive EMCCD camera suitable for fast in vivo imaging. It's one of the fastest and most sensitive system at the institute. The system can also scan multiple positions in the sample, with multiple channels in timelapse mode. In order to stay in focus it also includes a perfect focus system to correct for vertical drift during long movies.
System components
Lasers
| Type | Excitation line | Nominal power |
| Diode405 | 405nm | 100mW |
| DPSS488 | 488nm | 100mW |
| DPSS561 | 561nm | 50mW |
| Diode640 | 640nm | 100mW |
Objectives
| Magnification | Model | Immersion | NA | WD (mm) | PFS compatible | DIC compatible | PhC compatible | Reference |
| 10x | Nikon Plan DL | - | 0.25 | 10.5 | No | Yes (N1) | No | MRDxxxxx |
| 20x | Nikon Plan Fluor | MImm | 0.75 | 0.35 | Yes | Yes (N1) | No | MRDxxxxx |
| 40x | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | MRH01401 |
| 60x | Nikon Apo TIRF | Oil | 1.49 | 0.12 | Yes | Yes (N2) | No | MRDxxxxx |
| 63x | Nikon Plan APO VC | W | 1.20 | 0.175 | No | No | Yes | MRDxxxxx |
* Not fabricated anymore ; * * LWD - Long Working Distance
CSU Dichroics
Filters (CSU Filterwheel)
Camera(s)
Andor Spinning Disk W1 Quality Control
Andor Spinning Disk W1 Usage Statistics
