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andor_revolution

Location: No longer available (moved to CEDOC)
Manufacturer: Andor Technology
Model: Revolution XD
Year: 2009
SN: PU-0171
 
 
 
 
 
Andor Revolution Booking
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Andor Revolution FAQ  

System overview

The Andor Revolution spinning disk confocal microscope is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512×512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control and a small stage incubator for CO2 supply. A UV laser is attached to the back port of the microscope for spot laser ablation. With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the Zeiss Axiovert 200M. Even though its resolution is not as high as a point-scanning confocal system such as the Zeiss LSM 510 META or Zeiss LSM 710, it is much faster than these two systems. If you need to use an upright microscope, check the Zeiss LSM 5 Live fast confocal system instead. If your personal computer cannot handle all the data you collected, check out the Big Guy.

Interactive Flash Tutorial: Learn how to synchronize spinning disk rotation with camera exposure times

System components

LASERs

Laser Unit Wavelength Maximum Power Current Status
Diode CW 405 405 nm 50 mW ok
OPSL CW 488 488 nm 75 mW ok
DPSS 561 561 nm 75 mW ok
DPSS 640 640 nm 40 mW ok

Objectives

Magnification Model Type NA WD (mm) pixel size (μm) 10 μm =
10x Plan Fluor PFS Dry 0.30 16 1.43 7 pixels
20x Super Fluor PFS Dry 0.75 1 0.71 14 pixels
40x Plan Fluor PFS Oil 1.30 0.22 0.36 28 pixels
60x Plan Apo VC PFS Oil 1.40 0.13 0.24 42 pixels
100x Plan Fluor PFS Oil 1.30 0.20 0.14 70 pixels

Emission Filtersets

Setting Transmission
0: QUAD 420-460 + 511-531 + 590-624 + 678-722 nm
1: 685 665-705 nm
2: 605 579-631 nm
3: 525 500-550 nm
4: 447 417-477 nm
5: Block No Transmission
6: BF Empty - Full Transmission
7: analyzer DIC Polarizer
8: 568LP 581-1282 nm
9: 488LP 500-1100 nm

Microscope Turn On Procedure

  • Turn on the main cabinet switch

  • Turn on the stage controller and transmitted light power (if needed)

  • Turn on the Yokogawa spinning disk scanner

  • Turn on the Nikon microscope (button on the right side, near the back)

  • Turn on the fluorescence lamp (if needed)

  • Turn on the computer
  • Wait 2 min to allow for system components detection
  • Start the iQ software
  • Place your sample on the stage/piezo holder
  • Turn the Prior piezo control on

Microscope Turn Off Procedure

  • Turn off the piezo control
  • Remove your sample from the stage/piezo holder
  • Exit the iQ software
  • Switch off the computer
  • Turn off all system components (no particular order)

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andor_revolution.txt · Last modified: 2018/06/07 14:44 by bioimaging