about
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about [2019/12/31 17:29] bioimaging |
about [2025/06/18 01:40] (current) bioimaging |
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| - | ====== About the Bioimaging Unit ====== | + | ====== About the Bioimaging Platform ====== |
| - | The Bioimaging Unit acts as a support structure to help and nurture research done with Light Microscopy inside the institute. Besides managing resources, the Bioimaging Unit provides iMM scientists and visitors with excellence in scientific know-how and expertise in using light microscopy methods for their research. We assist in planning microscopy-oriented projects, choosing materials and equipment, analyzing experimental results, processing acquired images and presenting data. Together with continuous training of new users, we organize regular courses to introduce users to the most recent microscopy techniques and foster interactions and collaborations between microscopy users at the iMM. | + | <html> |
| + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=bioimaging_support.png" width=465 align=left> | ||
| + | </html> | ||
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| + | The Bioimaging Platform offers state-of-the-art **personalized support** in all steps of research done with Light Microscopy at GIMM, housing over 30 high-end systems including point-scanning confocal, spinning disk confocal, multiphoton, light sheet, widefield, slide scanning, and advanced image analysis workstations. | ||
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| + | **From the very first question to the moment of discovery and publication, we sit with researchers to advise, teach, train, and assist in image acquisition, analysis and processing, empowering scientists with cutting-edge technology and expertise.** | ||
| - | ===== Team ===== | + | In addition to daily hands-on training of users, we organize regular courses and workshops in basic and advanced light microscopy techniques and bioimage analysis. |
| - | {{:bioimaging_team.jpg?700|}} | + | We are a node of [[https://ppbi.pt/wordpress/|Portuguese Platform of Bioimaging (PPBI)]], a nationwide consortium of imaging facilities which integrates the European research infrastructure [[https://www.eurobioimaging.eu/|Euro-BioImaging ERIC]]. We are also a [[https://www.zeiss.com/microscopy/en/about-us/microscopy-customer-center.html|ZEISS labs@location Partner]], joining a community of advanced microscopy facilities providing in-depth knowledge, dedicated services across a wide range of applications and instrumentation, and scientific support to ZEISS customers. |
| <html> | <html> | ||
| - | <table style="border:0px solid white;"> | + | <p style="text-align:center"><a href="http://www.ppbi.pt/joomla30/" target="_blank"><img src="/facility/bioimaging/lib/exe/fetch.php?media=ppbi.png" width=140></a>   <a href="https://www.eurobioimaging.eu/" target="_blank"><img src="/facility/bioimaging/lib/exe/fetch.php?media=eurobioimaging.png" width=200></a>   <a href="https://www.zeiss.com/microscopy/en/about-us/microscopy-customer-center.html#partners" target="_blank"><img src="/facility/bioimaging/lib/exe/fetch.php?media=labslocation.png" width=200></a>   </p> |
| - | <td style="border:0px solid white;"> | + | |
| - | <div style="text-align: center;"><b>Head of Unit</b></div> | + | |
| - | <br> | + | |
| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/bioimaging/lib/exe/fetch.php?media=rino.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
| - | </td> | + | |
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| - | <span style="line-height:1.8"> | + | |
| - | <br> | + | |
| - | <br> | + | |
| - | <b>José Rino</b> has a <i>Licenciatura</i> in Technological Physics Engineering (Instituto Superior Técnico) and a PhD in Biophysics (Faculdade de Ciências). José has managed the Bioimaging Unit since January 2008 and was appointed Head in July 2009, after postdoctoral research at the iMM (Carmo-Fonseca Lab) focused on applying quantitative microscopy methods to study mRNA biogenesis. He is also Head of the Flow Cytometry Unit at iMM. <a href="https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=facility_bioimaging_doku.php&id=rino_cv">Click here for more information</a>.<br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>joserino@medicina.ulisboa.pt</i> | + | |
| - | </td> | + | |
| - | </table> | + | |
| </html> | </html> | ||
| + | |||
| + | ===== How to Acknowledge us ===== | ||
| <html> | <html> | ||
| - | <table style="border:0px solid white;"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=team_2025_right.png" width=320 align=right> |
| - | <tr style="border:0px solid white;"> | + | </html> |
| - | <td style="border:0px solid white;"> | + | |
| - | <div align="center"><b>Technical Support</b></div> | + | If you used our services, please acknowledge us in publications or other formal presentations. This is one of our key performance indicators and very important for future funding. You are welcome to use the following examples: |
| - | <br> | + | |
| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/bioimaging/lib/exe/fetch.php?media=lima.png" style="width:150px; height: 150px;" /></div><br><br> | + | ==== Materials and Methods ==== |
| - | </td> | + | “Microscopy experiments were performed at the Bioimaging Platform of the Gulbenkian Institute for Molecular Medicine.” |
| - | <td style="border:0px solid white;"> | + | |
| - | <span style="line-height:1.8"> | + | ==== Acknowledgements ==== |
| - | <br> | + | “We thank the technical support of the Bioimaging Platform of the Gulbenkian Institute for Molecular Medicine, funded by PPBI-POCI-01-0145-FEDER-022122.” |
| - | <br> | + | |
| - | <b>Aida Lima</b> has an Undergraduate degree in Biology and a MSc in Genetic Molecular Biology and Biomedicine from Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa. Aida joined the Bioimaging Unit in June 2019, after studying the dynamics of the actin cytoskeleton in endothelial tip cells using confocal microscopy, in Cláudio Franco's lab at the iMM. <br> | + | ==== Co-authorship ==== |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | Assistance provided above the technical or routine level, where the Platform staff provides scientific input and expertise in experimental set-up, acquisition, analysis or writing, should be recognized through co-authorship on resulting publications. Please check this [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=imaging_facility_guidelines_for_acknowledgements_all_logos_1_.pdf|Global Bioimaging poster]] for co-authorship guidelines and talk to us, we are happy to discuss possible co-authorship situations. |
| - | <i>aidalima@medicina.ulisboa.pt</i> | + | |
| - | </td> | + | Thank you! |
| - | </tr> | + | |
| - | <tr style="border:0px solid white;"> | + | <html> |
| - | <td style="border:0px solid white;"> | + | <p style="text-align:center"><img src="/facility/bioimaging/lib/exe/fetch.php?media=funding.png" width=850 align=center></p> |
| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nascimento.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
| - | </td> | + | |
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| - | <span style="line-height:1.8"> | + | |
| - | <b>Ana Nascimento</b> has a <i>Licenciatura</i> in Chemistry and a MSc in Biochemistry from Faculdade de Ciências. Ana joined the Bioimaging Unit in December 2012 after working as a flow cytometry technician and a microscopy technician at Instituto Gulbenkian de Ciência. <br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>anascimento@medicina.ulisboa.pt</i> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr style="border:0px solid white;"> | + | |
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| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/bioimaging/lib/exe/fetch.php?media=temudo.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
| - | </td> | + | |
| - | <td style="border:0px solid white;"> | + | |
| - | <span style="line-height:1.8"> | + | |
| - | <b>António Temudo</b> has a <i>Licenciatura</i> in Biology and a MSc in Evolutionary and Developmental Biology from Faculdade de Ciências. António joined the Bioimaging Unit in June 2009, after studying the mechanics of somitogenesis in vertebrate embryos (mouse and zebrafish) using confocal live imaging for his MSc thesis. António has also studied Medicine at the Faculdade de Ciências Médicas, Universidade Nova de Lisboa.<br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>atemudo@medicina.ulisboa.pt</i> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | </table> | + | |
| </html> | </html> | ||
| ===== Services ===== | ===== Services ===== | ||
| - | The Bioimaging Unit is equipped with 2 inverted laser point-scanning confocal microscopes prepared for live cell imaging ([[zeiss_lsm_880|Zeiss LSM 880]] and [[zeiss_lsm_710|Zeiss LSM 710]]), 1 lightsheet microscope prepared for water and clearing media immersion imaging ([[zeiss_lightsheet_z1|Zeiss Lightsheet Z1]]), 2 inverted spinning disk confocal microscopes with motorized stage, piezo and EM-CCD also prepared for live cell imaging ([[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]), 1 multi-photon microscope ([[leica_sp8_mp|Leica SP8 MP]]), 4 fully motorized inverted widefield microscopes with cooled CCD cameras and incubators ([[zeiss_cell_observer|Zeiss Cell Observer]], [[zeiss_axiovert_200m|Zeiss Axiovert 200M]], [[nikon_eclipse_ti|Nikon Eclipse Ti]] and [[nikon_eclipse_te2000e|Nikon Eclipse TE-2000-E]]), 1 upright widefield fluorescence microscope with a CCD camera ([[leica_dm5000b|Leica DM5000B]]), 2 fluorescence stereo microscopes with cooled CCD cameras ([[zeiss_lumar_v12|Zeiss Lumar V12]] and [[zeiss_axiozoom_v16|Zeiss AxioZoom V16]]) and other imaging resources such as a brightfield microscope ([[leica_dm2500|Leica DM2500]]) and a bioluminescent imaging system ([[ivis_lumina|IVIS Lumina]]). We also have two high-spec computers for image processing available for researchers to work on their imaging data ([[big_guy|Big Guy]] and [[colossus|Colossus]]). The Bioimaging systems are available on the [[https://imm.medicina.ulisboa.pt/intranet/booking/select_resource.php?v=1|online booking system]] for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience. | + | The Bioimaging Platform provides training and assistance in planning optical microscopy-oriented projects, choosing materials and equipment, preparing samples, acquiring data, analyzing and processing acquired images, presenting and publishing results. |
| - | → **More info on our** [[resources|{{resources_neg.png?0x35|}}]] | + | <html> |
| - | {{bioimaging_process_neg.png?800|}} | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=bioimaging_systems_2025.gif" width=340 align=left> |
| + | </html>The facility houses over 30 high-end systems, including five inverted laser point scanning confocal microscopes with motorized stage, piezo and super-resolution detector prepared for live cell imaging [3x [[https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-980-with-airyscan-2.html|ZEISS LSM 980 with Airyscan 2]] (one of them with a 32-channel spectral detector), [[zeiss_lsm_900|ZEISS LSM 900 with Airyscan 2]], [[zeiss_lsm_880|ZEISS LSM 880 with Airyscan]]), and one fully automated inverted widefield and point scanning confocal microscope optimized for high-throughput live imaging in well-plates ([[zeiss_celldiscoverer_7|ZEISS Celldiscoverer 7 with LSM 900]])], seven inverted spinning disk confocal microscopes with motorized stage, piezo and EM-CCD/sCMOS cameras prepared for live cell imaging [3x [[https://www.intelligent-imaging.com/marianas|3i Marianas SDC]] (one of them with TIRF and in a BSL2 room), [[https://andor.oxinst.com/products/dragonfly-confocal-microscope-system|Andor Dragonfly SDC]], [[andor_w1|Andor Spinning Disk W1]], [[leica_sdc|Leica Spinning Disk Confocal]], and [[zeiss_cell_observer_sd|ZEISS Cell Observer SD]]], three light sheet microscopes prepared for water and clearing media immersion imaging (2x ZEISS Lightsheet Z.1, and [[blaze|Miltenyi UltraMicroscope Blaze]]), two multi-photon microscopes ([[prairie|Prairie Multiphoton]] and [[https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-tcs-sp8-mp/|Leica SP8 MP]]), seven fully motorized inverted widefield microscopes with cooled CCD/CMOS cameras ([[https://www.agilent.com/en/product/cell-analysis/cell-imaging-microscopy/cell-imaging-multimode-readers/biotek-cytation-5-cell-imaging-multimode-reader-1623202|Agilent Cytation 5]] multimode reader with incubation, [[deltavision|Deltavision OMX-SIM]] super-resolution with incubation and SIM-TIRF, [[nikon_eclipse_ti|Nikon Eclipse Ti]] with stage top incubation and a Ionoptix calcium and contractility measurement system, [[nikon_htm-hcs|Nikon HTM-HCS]] with incubation, [[zeiss_cell_observer|ZEISS Cell Observer]] with a large cage incubator and an Akoya PhenoCycler for spatial phenotyping, [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-imager-2-for-life-science-research.html|ZEISS Imager with Apotome 2]] with 8 slide carrier, and ZEISS Axiovert 200M), one automated slide scanner for fluorescence and brightfield scanning up to 100 slides ([[https://www.zeiss.com/microscopy/en/products/imaging-systems/axioscan-for-biology.html|ZEISS Axioscan 7]]), two fluorescence stereo microscopes with CCD/CMOS cameras ([[https://www.zeiss.com/microscopy/en/products/light-microscopes/stereo-and-zoom-microscopes/axio-zoom-v16-for-biology.html|ZEISS Axio Zoom.V16]], and [[oeiras_zeiss_lumar_v12|ZEISS Stereo Lumar.V12]]) and other imaging resources such as a brightfield microscope ([[leica_dm2500|Leica DM2500]]) and a bioluminescence/fluorescence imaging system ([[ivis_lumina|IVIS Lumina]]). There are also high spec workstations for image analysis and processing ([[amira|Amira]], [[chronos|Chronos]], [[huygens|Huygens]], [[imaris|Imaris]], [[z2_workstation|Z2]], [[z3_workstation|Z3]], and ZEISS LSM 980-32 Workstations). | ||
| - | ===== Prices ===== | + | The Bioimaging systems are available on the [[https://gimm.agendoscience.com|online booking system]] for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience. |
| - | We charge according to booked time in each microscopy system. [[https://imm.medicina.ulisboa.pt/intranet/booking/select_resource.php?v=1|Online booking]] is mandatory for each usage session and cancellations are allowed if they are made prior to reserved time. Users that do not show up at their booked sessions will be charged in full. | + | → **More info on our** <html><a href="/facility/bioimaging/doku.php?id=resources"><img src="/facility/bioimaging/lib/exe/fetch.php?media=microscope_section.png" width=180></a></html> |
| - | → **Check our** [[billing|{{charge_rates_neg.png?0x35|}}]] | + | ===== Prices ===== |
| - | ===== Hours ===== | + | We charge according to booked time in each microscopy system. [[https://gimm.agendoscience.com/|Online booking]] is mandatory for each usage session and cancellations are allowed if they are made prior to reserved time. Users that do not show up at their booked sessions will be charged in full. |
| - | **9 a.m. - 6 p.m.** for **Training, Assistance and Consultations** | + | → **Check our** <html><a href="/facility/bioimaging/doku.php?id=billing"><img src="/facility/bioimaging/lib/exe/fetch.php?media=charges_section.png" width=150></a></html> |
| - | 24 hours, 7 days a week for Equipment Usage by **authorized, trained users** (see [[usage_policy|Usage Rules]]) | ||
| ===== How to use the service ===== | ===== How to use the service ===== | ||
| - | All new users must contact the Bioimaging Unit for training in each specific system. This also applies to researchers with previous experience in microscopy. Only trained users are allowed to book usage sessions on a particular microscope. | + | All new users must contact the Bioimaging Platform for training in each specific system. This also applies to researchers with previous experience in microscopy. Only trained users are allowed to book usage sessions on a particular microscope. |
| - | {{:users_over_years_neg.png?400|}} {{:usage_hours_neg.png?400|}} \\ | + | → **How to** <html><a href="/facility/bioimaging/doku.php?id=newuser"><img src="/facility/bioimaging/lib/exe/fetch.php?media=getting_started_section.png" width=180></a></html> |
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| - | → **Are you a** [[newuser|{{new_user_neg.png?0x35|}}]] | + | |
| ===== Contacts ===== | ===== Contacts ===== | ||
| - | Bioimaging Unit \\ | + | GIMM Bioimaging Platform \\ |
| - | **imm-bioimaging@medicina.ulisboa.pt** | + | **bioimaging@gimm.pt** |
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| + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_site.png" width=340 align=left><BR> | ||
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| + |     <b>GIMM Lisbon Site</b><BR> | ||
| + |     Edifício Egas Moniz<BR> | ||
| + |     Avenida Professor Egas Moniz<BR> | ||
| + |     1649-028 Lisboa, Portugal<BR> | ||
| + |     ☎ 47305 | ||
| + | </html> | ||
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| + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_site.png" width=250 align=left><BR> | ||
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| + |     <b>GIMM Oeiras Site</b><BR> | ||
| + |     Rua da Quinta Grande 6<BR> | ||
| + |     2780-156 Oeiras, Portugal<BR> | ||
| + |     ☎ 214464533<BR> | ||
| + |     ☎ 4533 | ||
| + | </html> | ||
| + | |||
| + | === Hours === | ||
| - | Instituto de Medicina Molecular, room P2-A-32 \\ | + | **9:00 - 17:30** (with a 1h break for lunch) for **Training, Assistance and Consultations** |
| - | Faculdade de Medicina de Lisboa \\ | + | |
| - | Av. Prof. Egas Moniz \\ | + | |
| - | 1649-028 Lisboa \\ | + | |
| - | {{bioimaging_map_neg.png?700|}} | + | 24 hours, 7 days a week for Equipment Usage by **authorized, trained users** (see [[usage_policy|Usage Policies]]) |
| - | + | ||
| - | {{phone_neg.png?20|}} Internal phone extension: 47222 | + | |
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| [[start|Back to the Bioimaging Wiki]] | [[start|Back to the Bioimaging Wiki]] | ||
about.1577809762.txt.gz · Last modified: 2019/12/31 17:29 by bioimaging
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