Bioimaging Wiki

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<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_map_new.png">[Lisbon] Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> 
<b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br>
<b>Model</b>: <a href="https://www.youtube.com/watch?v=sGTNBXq8omg">LSM 880 with Airyscan</a> (click for Zeiss webinar) <br>
<b>Nickname</b>: "880"<br>
<b>Software</b>: ZEN 2.3 SP1 (black edition) <br>
<b>Year</b>: 2016 <br>
<b>SN</b>: 2811000267 <br>
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Data will be deleted after: <b>1 month</b>
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&rarr; <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss LSM 880 Quality Control</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Zeiss LSM 880 Usage Statistics</a>
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===== Microscope overview =====

{{  :confocal_pinhole.png?340}} The Zeiss LSM 880 with Airyscan is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its Airyscan with 32 concentrically arranged GaAsP detection elements is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://www.youtube.com/watch?v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor 4x, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths).

  * [[https://www.youtube.com/watch?v=sGTNBXq8omg|ZEISS Webinar: LSM 880 with Airyscan - Revolutionize Your Confocal Imaging]]
  * [[https://www.youtube.com/watch?v=VuxgaKDa_DU|ZEISS Webinar: Fast Mode for ZEISS LSM 880 with Airyscan]]

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<p align="center"><iframe width="420" height="236" src="https://www.youtube.com/embed/QjKrLTHGnc8" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe> <iframe width="420" height="236" src="https://www.youtube.com/embed/ln6VUmLzLLc" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p>
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{{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.



===== System components =====

==== LASERs ====

^  Laser Unit  ^  Wavelength  ^  Maximum Power  ^
|  Violet  |  <fc #a600ff>405 nm</fc>  |  30 mW  |
|  Argon  |  <fc #0071ff>458</fc>, <fc #00f7ff>488</fc> and <fc #1aff00>514</fc> nm  |  25 mW  |
|  Yellow-green  |   <fc #c6ff00>561 nm</fc>  |  20 mW  |
|  Orange  |  <fc #ffd200>594 nm</fc>  |  2 mW  |
|  Red  |  <fc #ff4200>639 nm</fc>  |  5 mW  |

==== Filtersets (for eyepieces) ====

^  Position  ^  Filterset  ^  Excitation  ^  Dichroic  ^  Emission  ^  Reference  ^
|  2  |  Cyan  |  426-446 nm  |  455 nm  |  <fc #00d5ff>460-500 nm</fc>  |  [[https://www.chroma.com/products/sets/49001-et-ecfp|49001]]  |
|  3  |  Green  |  450-490 nm  |  493 nm  |  <fc #4aff00>500-550 nm</fc>  |  [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]]  |
|  4  |  Red  |  533-558 nm  |  570 nm  |  <fc #ffad00>570-640 nm</fc>  |  [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]]  |
|  5  |  Blue  |  G 365 nm  |  395 nm  |  <fc #0028ff>420-470 nm</fc>  |  [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]]  |


==== Objectives ====

^  Magnification   ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  Reference  ^
|  <fc #fef042>10x</fc>  |  EC Plan-Neofluar  |  Air  |  0.30  |  5.20  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]]  |
|  <fc #0aa05c>20x</fc>  |  Plan-Apochromat  |  Air  |  0.80  |  0.55  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]]  |
|  <fc #01b3f5>40x</fc>  |  C-Apochromat Corr  |  Water  |  1.20  |  0.28  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421767-9970-000/Objective-C-Apochromat-40x-1.20-W-Corr-M27|421767-9970-000]]  |
|  <fc #01b3f5>40x</fc>  |  LD C-Apochromat Corr  |  Water  |  1.10  |  0.62  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]]  |
|  <fc #2c80c0>63x</fc>  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]]  |


**Upon request:**

^  Magnification   ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  Reference  ^
|  <fc #0aa05c>25x</fc>  |  LCI Plan-Neofluar Corr DIC  |  Oil/Glyc/W  |  0.80  |  0.21  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]]  |
|  <fc #01b3f5>40x</fc>  |  Plan-Apochromat Corr  |  Dry  |  0.95  |  0.25  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]]  |

===== How to start your session =====

If you are the **first user** of the day:
  * Turn off Components
  * Turn off System/PC
  * Turn off Main Switch
{{zeiss_lsm_880_1.jpg?100}}
  * Turn on the computer
  * Turn on Main Switch
  * Turn on Systems/PC
  * Turn on Components
  * Turn on HXP Lamp
  * Log into Windows with your Agendo credentials
  * Start **ZEN Black**
Else:
  * Log into Windows with your Agendo credentials
  * Start **ZEN Black**

===== How to end your session =====

If there is another user:
  * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper)
  * Exit the ZEN software
  * Log off the computer
If you are the **last user** of the day:
  * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper)
  * Turn off LASERs on ZEN
  * Exit the ZEN software
  * Turn off HXP lamp
  * Turn off the computer

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