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zeiss_lsm_980_oeiras [2025/07/28 16:36] bioimaging [Booking Rules] |
zeiss_lsm_980_oeiras [2025/07/31 12:05] (current) bioimaging |
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| {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | ||
| - | ===== Booking Rules ===== | + | |
| - | - **Users** can book at maximum **8 hours per week** | + | |
| - | - This usage restriction does not apply for **weekends** and for working days **before 9:00** and **after 18:00** | + | |
| - | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | + | |
| ===== System components ===== | ===== System components ===== | ||
| Line 118: | Line 115: | ||
| The system should be always on, you only need to: | The system should be always on, you only need to: | ||
| * Turn on HXP (if needed) | * Turn on HXP (if needed) | ||
| - | * Turn on the computer (if needed) | + | {{::Oeiras_LSM980_hxp.png?100|}} |
| + | * If incubation is needed (turn on 30 mins before acquiring): | ||
| + | * Turn the microscope body off | ||
| + | {{::Oeiras_LSM980_microscope_body.png?150|}} | ||
| + | * Turn the incubation controller on | ||
| + | {{::Oeiras_LSM980_incubation.png?100|}} | ||
| + | * Turn the microscope body on | ||
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| * Start **ZEN** | * Start **ZEN** | ||
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| If there is another user for this microscope in the __next hour__: | If there is another user for this microscope in the __next hour__: | ||
| - | * Exit the ZEN software, leave the lasers on | + | * Exit ZEN, leave the lasers on |
| * Log off the computer | * Log off the computer | ||
| * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| Else: | Else: | ||
| - | * Turn off the lasers on **ZEN** | + | * Turn off the lasers in **ZEN** |
| * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| + | * Turn off HXP (if used) | ||
| + | * Turn off incubation controller (if used) | ||
| * Exit the ZEN software | * Exit the ZEN software | ||
| * Log off the computer | * Log off the computer | ||
| + | |||
| ===== Microscope Turn On Procedure (after complete shutdown) ===== | ===== Microscope Turn On Procedure (after complete shutdown) ===== | ||
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| If the system is completely off, please follow this procedure: | If the system is completely off, please follow this procedure: | ||
| - | * Turn on the ''MAIN SWITCH'' | + | * Turn on the ''Microscope power supply'' |
| - | {{::zeiss_lsm_980_main_switch.png?100|}} | + | * Turn on the ''Laser key'' |
| - | * Turn on the ''COMPONENTS'' switch | + | * Turn on ''HXP'' (if needed) |
| - | {{::zeiss_lsm_980_components.png?100|}} | + | * If ''incubation'' is needed (turn on 30 mins before acquiring): |
| - | * Turn on the Laser key | + | * Turn the microscope body off |
| - | * Turn on the computer | + | * Turn the incubation controller on |
| + | * Turn the microscope body on | ||
| + | * Turn on the ''computer'' | ||
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| - | * Start the **ZEN** software | + | * Start **ZEN** |
| ===== Microscope Turn Off Procedure (for complete shutdown) ===== | ===== Microscope Turn Off Procedure (for complete shutdown) ===== | ||
| Line 151: | Line 159: | ||
| Please only shutdown the system if instructed to do so by the Bioimaging Staff. | Please only shutdown the system if instructed to do so by the Bioimaging Staff. | ||
| - | * Turn off the lasers on **ZEN** | + | * Turn off the lasers in **ZEN** |
| - | * Exit the ZEN software | + | * Exit ZEN |
| * Shutdown the computer | * Shutdown the computer | ||
| - | * Turn off the Laser key | + | * Turn off the ''Laser key'' |
| - | * Turn off the ''COMPONENTS'' switch | + | * Turn off HXP (if used) |
| - | * Turn off the ''MAIN SWITCH'' | + | * Turn off the ''Microscope power supply'' |
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| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||