Bioimaging Wiki

The Zeiss LSM 980 with Airyscan 2 is a point scanning confocal microscope is an inverted microscope equipped with a laser scanning head with a total of 6 PMT/GaAsp + 1 Airyscan detectors. The Airyscan can achieve non-diffraction limited optical sectioning with resolutions down to 140nm laterally and ~400nm axially. It also allows acquisition in “Multiplexing” mode(s), which accelerate the acquisition speed (4/8x faster) or in sensitivity mode that enhances the signal-to-noise acquisition.

The microscope is also equipped with a dark heating and atmospheric control chamber to allow live imaging of mammalian cells (O2 control is also possible, upon request). We have available an objective inverter for some intra-vital or open-dish applications (upon request only, requires preparation).

• Microscope: Zeiss Axio Observer
• LED Illumination: Zeiss Colibri 7
• Features
  • | Definite Focus.3
  • | Airyscan 2 Multiplex
  • | LSM Plus
  • | Joint Deconvolution

• ZEISS Webinar: LSM 980 Airyscan 2 Multiplex mode

Data files older than 1 month will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.

If you require more laser lines (e.g., 445nm - CFP/Cerulean, 514nm - YFP, or 594nm mCherry/AF594), we suggest as an alternative the ZEISS LSM 980-32 in Lisbon.

*ask staff for help setting up the proper immersion medium setting

1x blue-green multialkali PMT (15-20% QE)

4x GaAsP PMT spectral detectors (tunable between 450-750 nm) (~45% QE)

1x green-red multialkali PMT (10-25% QE)

1x T-PMT detector (brighfield)

1x Airyscan2 detector (~45% QE after processing Airyscan data)

The system should be always on, you only need to:

• Turn on HXP (if needed)

• If incubation is needed (turn on 30 mins before acquiring):
  • Turn the microscope body off

• Turn the incubation controller on

• Turn the microscope body on
• Log into Windows with your Agendo credentials
• Start ZEN

If there is another user for this microscope in the next hour:

• Exit ZEN, leave the lasers on
• Log off the computer
• Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper)

Else:

• Turn off the lasers in ZEN
• Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper)
• Turn off HXP (if used)
• Turn off incubation controller (if used)
• Exit the ZEN software
• Log off the computer

If the system is completely off, please follow this procedure:

• Turn on the Microscope power supply
• Turn on the Laser key
• Turn on HXP (if needed)
• If incubation is needed (turn on 30 mins before acquiring):
  • Turn the microscope body off
  • Turn the incubation controller on
  • Turn the microscope body on
• Turn on the computer
• Log into Windows with your Agendo credentials
• Start ZEN

Please only shutdown the system if instructed to do so by the Bioimaging Staff.

• Turn off the lasers in ZEN
• Exit ZEN
• Shutdown the computer
• Turn off the Laser key
• Turn off HXP (if used)
• Turn off the Microscope power supply

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