Table of Contents
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Location: [Oeiras] Room 0B06 |
ZEISS LSM 980 with Airyscan 2 Quality Control
ZEISS LSM 980 with Airyscan 2 Usage Statistics![[Oeiras] Room 0B06](/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_zeiss_lsm_980.png){kind=link}
Microscope overview
The Zeiss LSM 980 with Airyscan 2 is an inverted point scanning confocal microscope equipped with a laser scanning head with a total of 6 PMT/GaAsp + 1 Airyscan detectors. The Airyscan can achieve non-diffraction limited optical sectioning with resolutions down to 140nm laterally and ~ 400nm axially. It also allows acquisition in “Multiplexing” modes, which accelerate the acquisition speed (4/8x faster) or in sensitivity mode that enhances the signal-to-noise acquisition.
The microscope is equipped with a dark large cage incubator and atmospheric control chamber to allow live imaging of mammalian cells (O2 control is also possible, upon request). We have available an objective inverter for some intra-vital or open-dish applications (upon request only, requires preparation).
- Microscope: Zeiss Axio Observer
- LED Illumination: Zeiss Colibri 7
- Features
- | LSM Plus
Data files older than 1 month will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.
System components
LASERs
| LASER | Wavelength | Maximum Power | Description (use) |
|---|---|---|---|
| violet | 405 nm | 15 mW | DAPI, Hoechst |
| blue | 488 nm | 13 mW | GFP, FITC, Alexa488 |
| yellow-green | 561 nm | 13 mW | RFP, TRITC, Cy3, Dylight Orange |
| red | 639 nm | 10 mW | Cy5, APC, Alexa/ATTO633-647 |
If you require more laser lines (e.g., 445 nm - CFP/Cerulean, 514 nm - YFP, or 594 nm mCherry/AF594), we suggest as an alternative the ZEISS LSM 980-32 with Airyscan 2 in Lisbon.
Filtersets (for eyepieces)
| Position | Excitation | Transmission | Fluorophores |
|---|---|---|---|
| DAPI | 385 nm | 445/50 nm | DAPI, CFP or Hoechst filter |
| GFP | 493 nm | 525/50 nm | GFP or FITC filter |
| RFP | 555 nm | LP590 nm | RFP or TRITC (LongPass) |
| Mirror | - | - | Mirror for LSM |
Objectives
| Magnification | Model | Immersion | NA | WD (mm) | Reference |
|---|---|---|---|---|---|
| 2.5x | - | 0.085 | 8.8 | 420320-9902-000 | |
| 10x | EC Plan-Neofluar | Air | 0.45 | 2.0 | 420640-9900-000 |
| 20x | Plan-Apochromat | Air | 0.75 | 0.55 | 420150-9900-000 |
| 25x | W/Sil/Gly/Oil * | 0.80 | 0.57 | 420852-9870-790 | |
| 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | 421867-9970-000 |
| 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | 420782-9900-799 |
* please ask staff for help setting up the proper immersion medium setting
Airyscan 2 emission filters
| Position | Emission |
|---|---|
| 1 | Plate |
| 2 | BP 420 - 480 + BP 495 - 550 |
| 3 | BP 420 - 470 + BP 570 - 630 |
| 4 | BP 420 - 500 + LP 605 |
| 5 | BP 465 - 505 + BP 525 - 585 |
| 6 | BP 495 - 550 + BP 570 - 630 |
| 7 | BP 495 - 555 + LP 660 |
| 8 | BP 570 - 620 + LP 660 |
Detectors
- 1x blue-green multialkali PMT (15-20% QE)
- 4x GaAsP PMT spectral detectors (tunable between 450-750 nm) (~45% QE)
- 1x green-red multialkali PMT (10-25% QE)
- 1x T-PMT detector (brighfield)
- 1x Airyscan2 detector (~45% QE after processing Airyscan data)
How to start your session
If you are the first user of the day:
- Turn off Main Switch
- Turn on the computer
- Turn on HXP, and incubation module (if needed)
- Turn on Main Switch
- Log in to Windows with your Agendo credentials
- Start ZEN
Else:
- Log in to Windows with your Agendo credentials
- Start ZEN
How to end your session
If there is another user:
- Clean up immersion objectives
- Exit the ZEN software
- Log off the computer
- Turn off HXP, and incubation module (if > 1 hour for the next user)
If you are the last user of the day:
- Clean up immersion objectives
- Exit the ZEN software
- Turn off the computer
- Turn off HXP, and incubation module (if used)
