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zeiss_lsm_980_oeiras [2025/07/19 22:03] bioimaging [Microscope Turn Off Procedure (for complete shutdown)] |
zeiss_lsm_980_oeiras [2025/07/31 12:05] (current) bioimaging |
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<b>Nickname</b>: "980"<br> | <b>Nickname</b>: "980"<br> | ||
- | <b>Software</b>: ZEN 3.3 <br> | + | <b>Software</b>: ZEN 3.12 <br> |
<b>Year</b>: 2019<br> | <b>Year</b>: 2019<br> | ||
<b>SN</b>: 2871000107<br> | <b>SN</b>: 2871000107<br> | ||
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{{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | ||
- | ===== Booking Rules ===== | + | |
- | - **Users** can book at maximum **8 hours per week** | + | |
- | - This usage restriction does not apply for **weekends** and for working days **before 9:00** and **after 21:00** | + | |
- | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | + | |
===== System components ===== | ===== System components ===== | ||
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The system should be always on, you only need to: | The system should be always on, you only need to: | ||
* Turn on HXP (if needed) | * Turn on HXP (if needed) | ||
- | * Turn on the computer (if needed) | + | {{::Oeiras_LSM980_hxp.png?100|}} |
+ | * If incubation is needed (turn on 30 mins before acquiring): | ||
+ | * Turn the microscope body off | ||
+ | {{::Oeiras_LSM980_microscope_body.png?150|}} | ||
+ | * Turn the incubation controller on | ||
+ | {{::Oeiras_LSM980_incubation.png?100|}} | ||
+ | * Turn the microscope body on | ||
* Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
* Start **ZEN** | * Start **ZEN** | ||
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If there is another user for this microscope in the __next hour__: | If there is another user for this microscope in the __next hour__: | ||
- | * Exit the ZEN software, leave the lasers on | + | * Exit ZEN, leave the lasers on |
* Log off the computer | * Log off the computer | ||
* Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
Else: | Else: | ||
- | * Turn off the lasers on **ZEN** | + | * Turn off the lasers in **ZEN** |
* Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
+ | * Turn off HXP (if used) | ||
+ | * Turn off incubation controller (if used) | ||
* Exit the ZEN software | * Exit the ZEN software | ||
* Log off the computer | * Log off the computer | ||
+ | |||
===== Microscope Turn On Procedure (after complete shutdown) ===== | ===== Microscope Turn On Procedure (after complete shutdown) ===== | ||
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If the system is completely off, please follow this procedure: | If the system is completely off, please follow this procedure: | ||
- | * Turn on the ''MAIN SWITCH'' | + | * Turn on the ''Microscope power supply'' |
- | {{::zeiss_lsm_980_main_switch.png?100|}} | + | * Turn on the ''Laser key'' |
- | * Turn on the ''COMPONENTS'' switch | + | * Turn on ''HXP'' (if needed) |
- | {{::zeiss_lsm_980_components.png?100|}} | + | * If ''incubation'' is needed (turn on 30 mins before acquiring): |
- | * Turn on the Laser key | + | * Turn the microscope body off |
- | * Turn on the computer | + | * Turn the incubation controller on |
+ | * Turn the microscope body on | ||
+ | * Turn on the ''computer'' | ||
* Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
- | * Start the **ZEN** software | + | * Start **ZEN** |
===== Microscope Turn Off Procedure (for complete shutdown) ===== | ===== Microscope Turn Off Procedure (for complete shutdown) ===== | ||
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Please only shutdown the system if instructed to do so by the Bioimaging Staff. | Please only shutdown the system if instructed to do so by the Bioimaging Staff. | ||
- | * Turn off the lasers on **ZEN** | + | * Turn off the lasers in **ZEN** |
- | * Exit the ZEN software | + | * Exit ZEN |
* Shutdown the computer | * Shutdown the computer | ||
- | * Turn off the Laser key | + | * Turn off the ''Laser key'' |
- | * Turn off the ''COMPONENTS'' switch | + | * Turn off HXP (if used) |
- | * Turn off the ''MAIN SWITCH'' | + | * Turn off the ''Microscope power supply'' |
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[[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] |