zeiss_lsm_980_oeiras
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zeiss_lsm_980_oeiras [2025/03/01 01:38] bioimaging [Objectives] |
zeiss_lsm_980_oeiras [2025/07/31 12:05] (current) bioimaging |
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| <b>Nickname</b>: "980"<br> | <b>Nickname</b>: "980"<br> | ||
| - | <b>Software</b>: Zen blue v3.3 <br> | + | <b>Software</b>: ZEN 3.12 <br> |
| - | <b>Year</b>: 2019 <br> | + | <b>Year</b>: 2019<br> |
| - | <b>SN</b>: <br> | + | <b>SN</b>: 2871000107<br> |
|   <br> |   <br> | ||
| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
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| {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | ||
| - | ===== Booking Rules ===== | + | |
| - | - **Users** can book at maximum **8 hours per week** | + | |
| - | - This usage restriction does not apply for **weekends** and for working days **before 9:00** and **after 21:00** | + | |
| - | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | + | |
| ===== System components ===== | ===== System components ===== | ||
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| ==== Lasers ===== | ==== Lasers ===== | ||
| - | ^ Laser ^ Excitation line ^ Power ^ Description (use) ^ | + | ^ Laser ^ Excitation line ^ Maximum Power ^ Description (use) ^ |
| | <fc #ff00ff>405 nm (violet)</fc> | 405 nm | 15 mW | <fc #00ffff>DAPI, Hoechst</fc> | | | <fc #ff00ff>405 nm (violet)</fc> | 405 nm | 15 mW | <fc #00ffff>DAPI, Hoechst</fc> | | ||
| | <fc #6495ed>488 nm (blue)</fc> | 488 nm | 13 mW | <fc #00ff00>GFP, FITC, Alexa488</fc> | | | <fc #6495ed>488 nm (blue)</fc> | 488 nm | 13 mW | <fc #00ff00>GFP, FITC, Alexa488</fc> | | ||
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| | <fc #ff0000>639 nm (red)</fc> | 639 nm | 10 mW | <fc #cd5c5c>Cy5, APC, Alexa/ATTO633-647</fc> | | | <fc #ff0000>639 nm (red)</fc> | 639 nm | 10 mW | <fc #cd5c5c>Cy5, APC, Alexa/ATTO633-647</fc> | | ||
| - | If you require more laser lines (eg, 445nm - CFP/Cerulean, 514nm - YFP, or 594nm mCherry/AF594), we suggest as an alternative the LSM980-32 in Lisbon: | + | If you require more laser lines (e.g., 445nm - CFP/Cerulean, 514nm - YFP, or 594nm mCherry/AF594), we suggest as an alternative the [[https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_lsm_980-32|ZEISS LSM 980-32]] in Lisbon. |
| - | https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_lsm_980 | + | |
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| 4x GaAsP PMT spectral detectors (tunable between 450-750 nm) (~45% QE) | 4x GaAsP PMT spectral detectors (tunable between 450-750 nm) (~45% QE) | ||
| - | 1x green-red multialkali PMT (10-45% QE) | + | 1x green-red multialkali PMT (10-25% QE) |
| 1x T-PMT detector (brighfield) | 1x T-PMT detector (brighfield) | ||
| - | 1x Airyscan2 detector (>>45% QE after processing Airyscan data) | + | 1x Airyscan2 detector (~45% QE after processing Airyscan data) |
| ==== Airyscan2 emission filters ===== | ==== Airyscan2 emission filters ===== | ||
| ^ Position ^ Emission ^ | ^ Position ^ Emission ^ | ||
| - | | 1 | | | + | | 1 | Plate | |
| - | | 2 | | | + | | 2 | BP 420 - 480 + BP 495 - 550 | |
| - | | 3 | | | + | | 3 | BP 420 - 470 + BP 570 - 630 | |
| - | | 4 | | | + | | 4 | BP 420 - 500 + LP 605 | |
| - | | 5 | | | + | | 5 | BP 465 - 505 + BP 525 - 585 | |
| - | | 6 | | | + | | 6 | BP 495 - 550 + BP 570 - 630 | |
| - | | 7 | | | + | | 7 | BP 495 - 555 + LP 660 | |
| - | | 8 | | | + | | 8 | BP 570 - 620 + LP 660 | |
| ==== Eyepiece filterset(s) (COLIBRI LED illumination) ===== | ==== Eyepiece filterset(s) (COLIBRI LED illumination) ===== | ||
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| - | ===== Turning ON ===== | + | ===== How to start your session ===== |
| - | 1. Verify if the fluorescent lamp is not hot (touch it), otherwise wait until it’s cold (10-15min). Afterwards, turn on the switch. NOTE. If Zen or the microscope are started with the fluorescence illuminator "OFF" they will not see it and need to be restarted afterwards. | + | The system should be always on, you only need to: |
| + | * Turn on HXP (if needed) | ||
| + | {{::Oeiras_LSM980_hxp.png?100|}} | ||
| + | * If incubation is needed (turn on 30 mins before acquiring): | ||
| + | * Turn the microscope body off | ||
| + | {{::Oeiras_LSM980_microscope_body.png?150|}} | ||
| + | * Turn the incubation controller on | ||
| + | {{::Oeiras_LSM980_incubation.png?100|}} | ||
| + | * Turn the microscope body on | ||
| + | * Log into Windows with your Agendo credentials | ||
| + | * Start **ZEN** | ||
| - | 2. Turn on the microscope and the Definite focus control box. | + | ===== How to end your session ===== |
| - | 3. Turn on laser key (turn laser key clockwise (or counter-clockwise?) to downward position) in the unit below the table. | + | If there is another user for this microscope in the __next hour__: |
| + | * Exit ZEN, leave the lasers on | ||
| + | * Log off the computer | ||
| + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| + | Else: | ||
| + | * Turn off the lasers in **ZEN** | ||
| + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| + | * Turn off HXP (if used) | ||
| + | * Turn off incubation controller (if used) | ||
| + | * Exit the ZEN software | ||
| + | * Log off the computer | ||
| - | 4. Login into your aGina account on the computer. Start ZEN Blue 3.3. | ||
| - | ===== Turning OFF ===== | + | ===== Microscope Turn On Procedure (after complete shutdown) ===== |
| - | DO NOT TURN OFF THE COMPONENTS SWITCH, AND THE STAGE AND PIEZO CONTROLLERS (UNDER THE TABLE) | + | If the system is completely off, please follow this procedure: |
| - | 1. Remove the sample and Gently wipe with optic cleaning paper and EtOH paper the immersion objective(s) used. If you suspect the objective needs deep cleaning please inform the staff. | + | * Turn on the ''Microscope power supply'' |
| + | * Turn on the ''Laser key'' | ||
| + | * Turn on ''HXP'' (if needed) | ||
| + | * If ''incubation'' is needed (turn on 30 mins before acquiring): | ||
| + | * Turn the microscope body off | ||
| + | * Turn the incubation controller on | ||
| + | * Turn the microscope body on | ||
| + | * Turn on the ''computer'' | ||
| + | * Log into Windows with your Agendo credentials | ||
| + | * Start **ZEN** | ||
| - | 2. Transfer your data to the institutional server (never connect USB sticks or disks on this workstation). | + | ===== Microscope Turn Off Procedure (for complete shutdown) ===== |
| - | 3. Check that the incubator(s) are off, unless they are needed by the next user (ask staff). | + | Please only shutdown the system if instructed to do so by the Bioimaging Staff. |
| - | 4. Close ZEN and logout from windows (if there’s an user after you, you can now leave the microscope). | + | * Turn off the lasers in **ZEN** |
| - | If there is no one in the next 2h, turn off the fluorescence lamp (behind the computer screen) and also exit Zen. | + | * Exit ZEN |
| - | If you are the last user of the day (check bookings in Agendo): Turn off Laser key (to horizontal position) in the large unit under the table and turn off the microscope and definite focus control box. | + | * Shutdown the computer |
| + | * Turn off the ''Laser key'' | ||
| + | * Turn off HXP (if used) | ||
| + | * Turn off the ''Microscope power supply'' | ||
| ---- | ---- | ||
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| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_lsm_980_oeiras.1740789481.txt.gz · Last modified: 2025/03/01 01:38 by bioimaging
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