zeiss_lsm_980_oeiras
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zeiss_lsm_980_oeiras [2025/03/01 01:38] bioimaging [Objectives] |
zeiss_lsm_980_oeiras [2025/05/16 01:02] (current) bioimaging |
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| <b>Nickname</b>: "980"<br> | <b>Nickname</b>: "980"<br> | ||
| - | <b>Software</b>: Zen blue v3.3 <br> | + | <b>Software</b>: ZEN 3.3 <br> |
| - | <b>Year</b>: 2019 <br> | + | <b>Year</b>: 2019<br> |
| - | <b>SN</b>: <br> | + | <b>SN</b>: 2871000107<br> |
|   <br> |   <br> | ||
| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
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| ==== Lasers ===== | ==== Lasers ===== | ||
| - | ^ Laser ^ Excitation line ^ Power ^ Description (use) ^ | + | ^ Laser ^ Excitation line ^ Maximum Power ^ Description (use) ^ |
| | <fc #ff00ff>405 nm (violet)</fc> | 405 nm | 15 mW | <fc #00ffff>DAPI, Hoechst</fc> | | | <fc #ff00ff>405 nm (violet)</fc> | 405 nm | 15 mW | <fc #00ffff>DAPI, Hoechst</fc> | | ||
| | <fc #6495ed>488 nm (blue)</fc> | 488 nm | 13 mW | <fc #00ff00>GFP, FITC, Alexa488</fc> | | | <fc #6495ed>488 nm (blue)</fc> | 488 nm | 13 mW | <fc #00ff00>GFP, FITC, Alexa488</fc> | | ||
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| | <fc #ff0000>639 nm (red)</fc> | 639 nm | 10 mW | <fc #cd5c5c>Cy5, APC, Alexa/ATTO633-647</fc> | | | <fc #ff0000>639 nm (red)</fc> | 639 nm | 10 mW | <fc #cd5c5c>Cy5, APC, Alexa/ATTO633-647</fc> | | ||
| - | If you require more laser lines (eg, 445nm - CFP/Cerulean, 514nm - YFP, or 594nm mCherry/AF594), we suggest as an alternative the LSM980-32 in Lisbon: | + | If you require more laser lines (e.g., 445nm - CFP/Cerulean, 514nm - YFP, or 594nm mCherry/AF594), we suggest as an alternative the [[https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_lsm_980-32|ZEISS LSM 980-32]] in Lisbon. |
| - | https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_lsm_980 | + | |
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| 4x GaAsP PMT spectral detectors (tunable between 450-750 nm) (~45% QE) | 4x GaAsP PMT spectral detectors (tunable between 450-750 nm) (~45% QE) | ||
| - | 1x green-red multialkali PMT (10-45% QE) | + | 1x green-red multialkali PMT (10-25% QE) |
| 1x T-PMT detector (brighfield) | 1x T-PMT detector (brighfield) | ||
| - | 1x Airyscan2 detector (>>45% QE after processing Airyscan data) | + | 1x Airyscan2 detector (~45% QE after processing Airyscan data) |
| ==== Airyscan2 emission filters ===== | ==== Airyscan2 emission filters ===== | ||
zeiss_lsm_980_oeiras.1740789481.txt.gz · Last modified: 2025/03/01 01:38 by bioimaging
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